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Rabbit Multiclonal c-Fos phospho T232 antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human FOS phospho T232.

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Images

Western blot - Anti-c-Fos (phospho T232) antibody [RP23040102] (AB308128), expandable thumbnail
  • Western blot - Anti-c-Fos (phospho T232) antibody [RP23040102] (AB308128), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: 99.91% PBS

Form

Liquid

Clonality

Multiclonal

Immunogen

  • Synthetic Peptide within Human FOS phospho T232. Database link P01100

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Tested

Tested
Tested

Species

Human

Dilution info

2-3 µg/mL

Notes

-

Target data

Function

Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation. In growing cells, activates phospholipid synthesis, possibly by activating CDS1 and PI4K2A. This activity requires Tyr-dephosphorylation and association with the endoplasmic reticulum.

Alternative names

Proto-oncogene c-Fos, Cellular oncogene fos, G0/G1 switch regulatory protein 7, FOS, G0S7

Recommended products

Rabbit Multiclonal c-Fos phospho T232 antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human FOS phospho T232.

Alternative names

Proto-oncogene c-Fos, Cellular oncogene fos, G0/G1 switch regulatory protein 7, FOS, G0S7

Key facts

Isotype

IgG

Form

Liquid

Clonality

Multiclonal

Immunogen
  • Synthetic Peptide within Human FOS phospho T232. Database link P01100
Clone number

RP23040102

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains.

Recombinant multiclonal antibodies offer the sensitivity of polyclonal antibodies by recognising multiple epitopes, along with consistency of a recombinant antibody.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The c-Fos protein also known as c-Fos is a component of the Fos family of transcription factors which includes FosB Fra-1 and Fra-2. These proteins play a role in regulating gene expression. c-Fos has a molecular weight of about 55 kDa. You usually find c-Fos expressed in various tissues including the brain providing a marker for neuronal activity and is often induced in response to stimuli like stress growth factors and mitogens. In research settings c-Fos staining and c-Fos immunohistochemistry are techniques commonly used to study activation patterns in cells and tissues.

Biological function summary

C-Fos acts as part of the Activator Protein-1 (AP-1) complex a group of DNA-binding proteins that regulate gene transcription. This complex forms when c-Fos and Jun proteins dimerize. The AP-1 complex controls several cellular processes such as proliferation differentiation and apoptosis. Due to its participation in these vital processes c-Fos influences cellular responses to external stimuli and can affect how cells behave under stress.

Pathways

C-Fos significantly contributes to the MAPK/ERK pathway which plays a role in cell growth and differentiation. It partners with Jun proteins to form functional transcription factors within the MAPK signaling cascade. Additionally c-Fos is involved in the JNK signaling pathway linking it to stress responses and apoptosis. These pathways highlight the partnerships between c-Fos and other proteins like ERK and JNK indicating their intertwined roles in cellular signaling networks.

Associated diseases and disorders

Researchers associate c-Fos with cancer and neurodegenerative diseases. In cancer c-Fos expression correlates with changes in AP-1 activity which can lead to altered cell growth and proliferation potentially contributing to tumor formation. Its involvement in neurodegenerative diseases reveals its role in neuron apoptosis and cellular stress responses. In these scenarios proteins like Bcl-2 also play a part with CDK1 sharing connections in processes that affect cell cycle and apoptosis illustrating c-Fos's broad significance in both normal and pathological conditions.

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2 product images

  • Western blot - Anti-c-Fos (phospho T232) antibody [RP23040102] (ab308128), expandable thumbnail

    Western blot - Anti-c-Fos (phospho T232) antibody [RP23040102] (ab308128)

    Blot B. To confirm the specificity of ab308128, competition was performed with the phosphopeptide (10 µg/mL) as shown. The peptide competes with the antibody and prevents it from binding to the membrane. Known quantity of protein samples were electrophoresed using a 4-12% Bis-Tris gel, electrophoresis system and pre-stained protein standard. Resolved proteins were then transferred onto a nitrocellulose membrane. The membrane was probed with the relevant primary and secondary antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed (ECL).

    All lanes: Western blot - Anti-c-Fos (phospho T232) antibody [RP23040102] (ab308128) at 2 µg/mL

    Lane 1: HeLa cell lysate with phosphopeptide at 30 µg

    Lane 2: HeLa cell lysate treated with EGF and phosphopeptide at 30 µg

    Lane 3: A431 cell lysate with phosphopeptide at 30 µg

    Lane 4: A431 cell lysate treated with EGF and phosphopeptide at 30 µg

    Secondary

    All lanes: Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/2500 dilution

    Developed using the ECL technique.

    Predicted band size: 41 kDa

  • Western blot - Anti-c-Fos (phospho T232) antibody [RP23040102] (ab308128), expandable thumbnail

    Western blot - Anti-c-Fos (phospho T232) antibody [RP23040102] (ab308128)

    Blot A. Western blot analysis was performed on whole cell extracts (30 µg lysate) of HeLa (lane 1), HeLa treated with EGF (200 µg/mL/10 min), A-431 (lane 3) and A-431 treated with EGF (200 µg/mL/10 min) (lane 4). The blots were probed with ab308128, 2-3 µg/mL and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate (0.4 µg/mL, 1:2500 dilution). A clear 40 kDa band corresponding to c-Fos (phospho T232) was observed across cell lines tested. Known quantity of protein samples were electrophoresed using a 4-12% Bis-Tris gel, electrophoresis system and pre-stained protein standard. Resolved proteins were then transferred onto a nitrocellulose membrane. The membrane was probed with the relevant primary and secondary antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed (ECL).

    All lanes: Western blot - Anti-c-Fos (phospho T232) antibody [RP23040102] (ab308128) at 2 µg/mL

    Lane 1: HeLa cell lysate at 30 µg

    Lane 2: HeLa cell lysate treated with EGF (200 µg/mL/10 min) at 30 µg

    Lane 3: A431 cell lysate at 30 µg

    Lane 4: A-431 cell lysate treated with EGF (200 µg/mL/10 min) at 30 µg

    Secondary

    All lanes: Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP-conjugate at 1/2500 dilution

    Developed using the ECL technique.

    Predicted band size: 41 kDa

    Observed band size: 40 kDa

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