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AB27793

Anti-c-Fos (phospho T325) antibody

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(1 Review)

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(14 Publications)

Rabbit Polyclonal c-Fos phospho T325 antibody. Suitable for ChIP, WB, ICC/IF and reacts with Human samples. Cited in 14 publications. Immunogen corresponding to Synthetic Peptide within Human FOS phospho T325.

View Alternative Names

G0S7, FOS, Protein c-Fos, Cellular oncogene fos, G0/G1 switch regulatory protein 7, Proto-oncogene c-Fos, Transcription factor AP-1 subunit c-Fos

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-c-Fos (phospho T325) antibody (AB27793)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-c-Fos (phospho T325) antibody (AB27793)

Immunofluorescence analysis of c-Fos (phospho T325) was done on 70% confluent log phase HeLa cell treated with 200 nM of PMA for 20 minutes. The cell were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Phosc-Fos (phospho T325)Rabbit Polyclonal Antibody (ab27793) at 2 ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1/2000 for 45 minutes at room temperature (Panel a : green). Nuclei (Panel b : blue) were stained with DAPI. F-actin (Panel c : red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin. Panel d is a merged image showing nuclear and cytoplasmic localization. Panel e is untreated cell with no signal. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

ChIP - Anti-c-Fos (phospho T325) antibody (AB27793)
  • ChIP

Supplier Data

ChIP - Anti-c-Fos (phospho T325) antibody (AB27793)

Chromatin Immunoprecipitation (ChIP) was performed using Anti-c-Fos (phospho T325) antibody (ab27793) 3 ug on sheared chromatin from 2 million A431 cells treated with EGF (200ng/ml), for 30 minutes. Normal Rabbit IgG was used as a negative IP control. The purified DNA was analyzed by 7500 Fast qPCR system with optimized PCR primer pairs for the promoter of active IL-6, CDKN1A gene, used as positive control target, and the SAT2, used as negative control target. Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.

Western blot - Anti-c-Fos (phospho T325) antibody (AB27793)
  • WB

Unknown

Western blot - Anti-c-Fos (phospho T325) antibody (AB27793)

The figure shows that the phosphorylation of cFos on threonine 325 is induced by EGF treatment and that Lambda phosphatase treatment eliminates the signal, thereby demonstrating the phospho specificity of ab27793.

All lanes:

Western blot - Anti-c-Fos (phospho T325) antibody (ab27793) at 1/1000 dilution

Lane 1:

Non EGF treated A431 cell lysate

Lane 2:

EGF treated A431 cell lysate

Lane 3:

EGF treated A431 cell lysate treated with Lambda phosphatase

Secondary

All lanes:

goat F(ab’)2 anti rabbit IgG HRP conjugate

Predicted band size: 41 kDa

Observed band size: 58 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF, ChIP

applications

Immunogen

Synthetic Peptide within Human FOS phospho T325. The exact immunogen used to generate this antibody is proprietary information.

P01100

Specificity

ab27793 recognises the cFos phosphorilated at threonine 325 form.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "1-3 µg/cells for 6 µg/cells", "ChIP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab27793 was negatively preadsorbed using a non phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non phosphorylated cFos. Immunogen affinity purification followed.
Storage buffer
pH: 7.3 Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The c-Fos protein also known as c-Fos is a component of the Fos family of transcription factors which includes FosB Fra-1 and Fra-2. These proteins play a role in regulating gene expression. c-Fos has a molecular weight of about 55 kDa. You usually find c-Fos expressed in various tissues including the brain providing a marker for neuronal activity and is often induced in response to stimuli like stress growth factors and mitogens. In research settings c-Fos staining and c-Fos immunohistochemistry are techniques commonly used to study activation patterns in cells and tissues.
Biological function summary

C-Fos acts as part of the Activator Protein-1 (AP-1) complex a group of DNA-binding proteins that regulate gene transcription. This complex forms when c-Fos and Jun proteins dimerize. The AP-1 complex controls several cellular processes such as proliferation differentiation and apoptosis. Due to its participation in these vital processes c-Fos influences cellular responses to external stimuli and can affect how cells behave under stress.

Pathways

C-Fos significantly contributes to the MAPK/ERK pathway which plays a role in cell growth and differentiation. It partners with Jun proteins to form functional transcription factors within the MAPK signaling cascade. Additionally c-Fos is involved in the JNK signaling pathway linking it to stress responses and apoptosis. These pathways highlight the partnerships between c-Fos and other proteins like ERK and JNK indicating their intertwined roles in cellular signaling networks.

Researchers associate c-Fos with cancer and neurodegenerative diseases. In cancer c-Fos expression correlates with changes in AP-1 activity which can lead to altered cell growth and proliferation potentially contributing to tumor formation. Its involvement in neurodegenerative diseases reveals its role in neuron apoptosis and cellular stress responses. In these scenarios proteins like Bcl-2 also play a part with CDK1 sharing connections in processes that affect cell cycle and apoptosis illustrating c-Fos's broad significance in both normal and pathological conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation. In growing cells, activates phospholipid synthesis, possibly by activating CDS1 and PI4K2A. This activity requires Tyr-dephosphorylation and association with the endoplasmic reticulum.
See full target information FOS phospho T325
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Publications (14)

Recent publications for all applications. Explore the full list and refine your search

Journal of ovarian research 16:144 PubMed37480147

2023

Transcription factor c-fos induces the development of premature ovarian insufficiency by regulating MALAT1/miR-22-3p/STAT1 network.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Qiu,Jie Zhou,Bing Ji,Liuyang Yuan,Tingsong Weng,Huishu Liu

Bone research 10:24 PubMed35232979

2022

Sialylation of TLR2 initiates osteoclast fusion.

Applications

Unspecified application

Species

Unspecified reactive species

Ce Dou,Gehua Zhen,Yang Dan,Mei Wan,Nathachit Limjunyawong,Xu Cao

BMC complementary medicine and therapies 22:52 PubMed35227255

2022

Extract of Corallodiscus flabellata attenuates renal fibrosis in SAMP8 mice via the Wnt/β-catenin/RAS signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Bing Cao,Mengnan Zeng,Yanpo Si,Beibei Zhang,Yangyang Wang,Ruiqi Xu,Yanjie Huang,Weisheng Feng,Xiaoke Zheng

Biology 11: PubMed35053118

2022

Konjac Ceramide (kCer)-Mediated Signal Transduction of the Sema3A Pathway Promotes HaCaT Keratinocyte Differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Seigo Usuki,Noriko Tamura,Tomohiro Tamura,Kohei Yuyama,Daisuke Mikami,Katsuyuki Mukai,Yasuyuki Igarashi

Scientific reports 11:21159 PubMed34707101

2021

NRIP1 is activated by C-JUN/C-FOS and activates the expression of PGR, ESR1 and CCND1 in luminal A breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Renata Binato,Stephany Corrêa,Carolina Panis,Gerson Ferreira,Igor Petrone,Igor Rodrigues da Costa,Eliana Abdelhay

Molecular medicine reports 24: PubMed34278468

2021

Psoralen inhibits the inflammatory response and mucus production in allergic rhinitis by inhibiting the activator protein 1 pathway and the downstream expression of cystatin‑SN.

Applications

Unspecified application

Species

Unspecified reactive species

Wenying Gao,Zhenglong Jin,Yanxia Zheng,Youjia Xu

Journal of biochemical and molecular toxicology 34:e22493 PubMed32319158

2020

Bilobalide assuages morphine-induced addiction in hippocampal neuron cells through upregulation of microRNA-101.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Zhang,Hongyin Zhai

Chemico-biological interactions 315:108866 PubMed31669319

2019

WITHDRAWN: Bisphenol S triggers the malignancy of hemangioma cells via regulation of basic fibroblast growth factor.

Applications

Unspecified application

Species

Unspecified reactive species

Dahai Liu,Yubo Hu,Junrong Wang,Cong Ye,Jianshi Du

International journal of oncology 54:443-454 PubMed30483762

2018

MicroRNA-30e inhibits adhesion, migration, invasion and cell cycle progression of prostate cancer cells via inhibition of the activation of the MAPK signaling pathway by downregulating CHRM3.

Applications

Unspecified application

Species

Unspecified reactive species

Xin-Min Zheng,Peng Zhang,Man-Hua Liu,Ping Chen,Wei-Bing Zhang

Oncotarget 8:38767-38779 PubMed28404976

2017

Estrogen enhances mismatch repair by induction of MLH1 expression via estrogen receptor-β.

Applications

Unspecified application

Species

Unspecified reactive species

Jun-Yu Lu,Peng Jin,Wei Gao,De-Zhi Wang,Jian-Qiu Sheng
View all publications
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