Rabbit Polyclonal c-Fos phospho T325 antibody. Suitable for ChIP, WB, ICC/IF and reacts with Human samples. Cited in 12 publications. Immunogen corresponding to Synthetic Peptide within Human FOS phospho T325.
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- Bone research 10:242022Sialylation of TLR2 initiates osteoclast fusion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCe Dou,Gehua Zhen,Yang Dan,Mei Wan,Nathachit Limjunyawong,Xu CaoPubMed 35232979
- BMC complementary medicine and therapies 22:522022Extract of Corallodiscus flabellata attenuates renal fibrosis in SAMP8 mice via the Wnt/β-catenin/RAS signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBing Cao,Mengnan Zeng,Yanpo Si,Beibei Zhang,Yangyang Wang,Ruiqi Xu,Yanjie Huang,Weisheng Feng,Xiaoke ZhengPubMed 35227255
- Biology 11:2022Konjac Ceramide (kCer)-Mediated Signal Transduction of the Sema3A Pathway Promotes HaCaT Keratinocyte Differentiation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSeigo Usuki,Noriko Tamura,Tomohiro Tamura,Kohei Yuyama,Daisuke Mikami,Katsuyuki Mukai,Yasuyuki IgarashiPubMed 35053118
- Scientific reports 11:211592021NRIP1 is activated by C-JUN/C-FOS and activates the expression of PGR, ESR1 and CCND1 in luminal A breast cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRenata Binato,Stephany Corrêa,Carolina Panis,Gerson Ferreira,Igor Petrone,Igor Rodrigues da Costa,Eliana AbdelhayPubMed 34707101
- Molecular medicine reports 24:2021Psoralen inhibits the inflammatory response and mucus production in allergic rhinitis by inhibiting the activator protein 1 pathway and the downstream expression of cystatin‑SN.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenying Gao,Zhenglong Jin,Yanxia Zheng,Youjia XuPubMed 34278468
- Chemico-biological interactions 315:1088662019WITHDRAWN: Bisphenol S triggers the malignancy of hemangioma cells via regulation of basic fibroblast growth factor.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDahai Liu,Yubo Hu,Junrong Wang,Cong Ye,Jianshi DuPubMed 31669319
- International journal of oncology 54:443-4542018MicroRNA-30e inhibits adhesion, migration, invasion and cell cycle progression of prostate cancer cells via inhibition of the activation of the MAPK signaling pathway by downregulating CHRM3.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXin-Min Zheng,Peng Zhang,Man-Hua Liu,Ping Chen,Wei-Bing ZhangPubMed 30483762
- Oncotarget 8:38767-387792017Estrogen enhances mismatch repair by induction of MLH1 expression via estrogen receptor-β.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJun-Yu Lu,Peng Jin,Wei Gao,De-Zhi Wang,Jian-Qiu ShengPubMed 28404976
- Oncology reports 34:1449-592015Topical treatment with Xiaozheng Zhitong Paste alleviates bone cancer pain by inhibiting proteinase-activated receptor 2 signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYanju Bao et. al.PubMed 26133236
- Cell 141:884-962010Ligand-specific c-Fos expression emerges from the spatiotemporal control of ErbB network dynamics.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.3
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human FOS phospho T325. The exact immunogen used to generate this antibody is proprietary information. Database link P01100
Reactivity data
Select an application| ChIP | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
ChIP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-3.00000 µg/cells for 6 µg/cells | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/250 | Notes - |
Associated Products
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6 products for Alternative Product
Target data
Function
Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation. In growing cells, activates phospholipid synthesis, possibly by activating CDS1 and PI4K2A. This activity requires Tyr-dephosphorylation and association with the endoplasmic reticulum.
Alternative names
G0S7, FOS, Protein c-Fos, Cellular oncogene fos, G0/G1 switch regulatory protein 7, Proto-oncogene c-Fos, Transcription factor AP-1 subunit c-Fos
Recommended products
Rabbit Polyclonal c-Fos phospho T325 antibody. Suitable for ChIP, WB, ICC/IF and reacts with Human samples. Cited in 12 publications. Immunogen corresponding to Synthetic Peptide within Human FOS phospho T325.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.3
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human FOS phospho T325. The exact immunogen used to generate this antibody is proprietary information. Database link P01100
- Purification technique
- Affinity purification Immunogen
- Specificity
ab27793 recognises the cFos phosphorilated at threonine 325 form.
- Concentration
- Purification notes
ab27793 was negatively preadsorbed using a non phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non phosphorylated cFos. Immunogen affinity purification followed.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The c-Fos protein also known as c-Fos is a component of the Fos family of transcription factors which includes FosB Fra-1 and Fra-2. These proteins play a role in regulating gene expression. c-Fos has a molecular weight of about 55 kDa. You usually find c-Fos expressed in various tissues including the brain providing a marker for neuronal activity and is often induced in response to stimuli like stress growth factors and mitogens. In research settings c-Fos staining and c-Fos immunohistochemistry are techniques commonly used to study activation patterns in cells and tissues.
- Biological function summary
C-Fos acts as part of the Activator Protein-1 (AP-1) complex a group of DNA-binding proteins that regulate gene transcription. This complex forms when c-Fos and Jun proteins dimerize. The AP-1 complex controls several cellular processes such as proliferation differentiation and apoptosis. Due to its participation in these vital processes c-Fos influences cellular responses to external stimuli and can affect how cells behave under stress.
- Pathways
C-Fos significantly contributes to the MAPK/ERK pathway which plays a role in cell growth and differentiation. It partners with Jun proteins to form functional transcription factors within the MAPK signaling cascade. Additionally c-Fos is involved in the JNK signaling pathway linking it to stress responses and apoptosis. These pathways highlight the partnerships between c-Fos and other proteins like ERK and JNK indicating their intertwined roles in cellular signaling networks.
- Associated diseases and disorders
Researchers associate c-Fos with cancer and neurodegenerative diseases. In cancer c-Fos expression correlates with changes in AP-1 activity which can lead to altered cell growth and proliferation potentially contributing to tumor formation. Its involvement in neurodegenerative diseases reveals its role in neuron apoptosis and cellular stress responses. In these scenarios proteins like Bcl-2 also play a part with CDK1 sharing connections in processes that affect cell cycle and apoptosis illustrating c-Fos's broad significance in both normal and pathological conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-c-Fos (phospho T325) antibody (ab27793)
The figure shows that the phosphorylation of cFos on threonine 325 is induced by EGF treatment and that Lambda phosphatase treatment eliminates the signal, thereby demonstrating the phospho specificity of ab27793.
All lanes: Western blot - Anti-c-Fos (phospho T325) antibody (ab27793) at 1/1000 dilution
Lane 1: Non EGF treated A431 cell lysate
Lane 2: EGF treated A431 cell lysate
Lane 3: EGF treated A431 cell lysate treated with Lambda phosphatase
SecondaryAll lanes: goat F(ab’)2 anti rabbit IgG HRP conjugate
Predicted band size: 41 kDa
Observed band size: 58 kDa
ChIP - Anti-c-Fos (phospho T325) antibody (ab27793)
Chromatin Immunoprecipitation (ChIP) was performed using Anti-c-Fos (phospho T325) antibody (ab27793) 3 ug on sheared chromatin from 2 million A431 cells treated with EGF (200ng/ml), for 30 minutes. Normal Rabbit IgG was used as a negative IP control. The purified DNA was analyzed by 7500 Fast qPCR system with optimized PCR primer pairs for the promoter of active IL-6, CDKN1A gene, used as positive control target, and the SAT2, used as negative control target. Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.
Immunocytochemistry/ Immunofluorescence - Anti-c-Fos (phospho T325) antibody (ab27793)
Immunofluorescence analysis of c-Fos (phospho T325) was done on 70% confluent log phase HeLa cell treated with 200 nM of PMA for 20 minutes. The cell were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Phosc-Fos (phospho T325)Rabbit Polyclonal Antibody (ab27793) at 2 ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1/2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin. Panel d is a merged image showing nuclear and cytoplasmic localization. Panel e is untreated cell with no signal. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
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