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AB25939

Anti-c-IAP1 and c-IAP2 antibody

4

(1 Review)

|

(9 Publications)

Rabbit Polyclonal c-IAP1 and c-IAP2 antibody. Suitable for WB, IHC-P and reacts with Human, Rat samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human BIRC2.

View Alternative Names

API1, MIHB, RNF48, BIRC2, Baculoviral IAP repeat-containing protein 2, Cellular inhibitor of apoptosis 1, IAP homolog B, Inhibitor of apoptosis protein 2, RING finger protein 48, RING-type E3 ubiquitin transferase BIRC2, TNFR2-TRAF-signaling complex protein 2, C-IAP1, hIAP-2, hIAP2

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-IAP1 and c-IAP2 antibody (AB25939)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-IAP1 and c-IAP2 antibody (AB25939)

Immunofluorescence of cIAP in Human Lung cells using ab25939 at 20 ug/ml.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-IAP1 and c-IAP2 antibody (AB25939)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-IAP1 and c-IAP2 antibody (AB25939)

Immunohistochemical analysis of paraffin-embedded human lung tissue using ab25939 at 10 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1h at RT. Antigen retrieval was by heat mediation with a citrate buffer (pH 6.0). Samples were incubated with ab25939 overnight at 4°C. Goat anti-rabbit IgG H&L (HRP) was used as a secondary at 1/250 dilution. Counter stained with Hematoxylin.

Western blot - Anti-c-IAP1 and c-IAP2 antibody (AB25939)
  • WB

Unknown

Western blot - Anti-c-IAP1 and c-IAP2 antibody (AB25939)

Blocking buffer : 5% NFDM/TBST.

Lane 1:

Western blot - Anti-c-IAP1 and c-IAP2 antibody (ab25939) at 1 µg/mL

Lane 2:

Western blot - Anti-c-IAP1 and c-IAP2 antibody (ab25939) at 2 µg/mL

Lane 3:

Western blot - Anti-c-IAP1 and c-IAP2 antibody (ab25939) at 4 µg/mL

All lanes:

Human lung lysate at 15 µg

Secondary

All lanes:

Goat anti-rabbit IgG HRP Conjugate at 1/10000 dilution

false

Western blot - Anti-c-IAP1 and c-IAP2 antibody (AB25939)
  • WB

Unknown

Western blot - Anti-c-IAP1 and c-IAP2 antibody (AB25939)

Lane 1:

Western blot - Anti-c-IAP1 and c-IAP2 antibody (ab25939) at 1 µg/mL

Lane 2:

Western blot - Anti-c-IAP1 and c-IAP2 antibody (ab25939) at 2 µg/mL

Lane 3:

Western blot - Anti-c-IAP1 and c-IAP2 antibody (ab25939) at 4 µg/mL

All lanes:

Human lung lysate

false

Western blot - Anti-c-IAP1 and c-IAP2 antibody (AB25939)
  • WB

Supplier Data

Western blot - Anti-c-IAP1 and c-IAP2 antibody (AB25939)

All lanes:

Western blot - Anti-c-IAP1 and c-IAP2 antibody (ab25939) at 1 µg/mL

Lane 1:

Human Thymus at 15 µg

Lane 2:

Human Lung at 15 µg

Lane 3:

Human Testis at 15 µg

Lane 4:

Human Small Intestine at 15 µg

Lane 5:

Rat Brain at 15 µg

Secondary

All lanes:

Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution

false

Western blot - Anti-c-IAP1 and c-IAP2 antibody (AB25939)
  • WB

Supplier Data

Western blot - Anti-c-IAP1 and c-IAP2 antibody (AB25939)

All lanes:

Western blot - Anti-c-IAP1 and c-IAP2 antibody (ab25939) at 2 µg/mL

Lane 1:

HEK293 cell lysates at 15 µg

Lane 2:

K562 cell lysates at 15 µg

Lane 3:

A549 cell lysates at 15 µg

Secondary

All lanes:

Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat, Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human BIRC2. The exact immunogen used to generate this antibody is proprietary information.

Q13490

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "10-20 µg/mL", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-1 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
cIAP Antibody is affinity chromatography purified via peptide column.
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle|Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

C-IAP1 and c-IAP2 also known as cellular inhibitors of apoptosis proteins 1 and 2 are important regulators of apoptosis and inflammation. c-IAP1 has a molecular weight of approximately 70 kDa while c-IAP2 is around 74 kDa. Both proteins are expressed in many tissues including the heart liver and immune cells. They function as E3 ubiquitin ligases where they target specific proteins for ubiquitination leading to proteasomal degradation. This activity plays a significant role in controlling cell survival and inflammation by modulating the levels of key signaling proteins.
Biological function summary

C-IAP1 and c-IAP2 contribute to cell survival by inhibiting apoptosis. They are part of a complex that interacts with other proteins and signaling molecules to regulate the apoptotic machinery. c-IAP1 and c-IAP2 interact with members of the tumor necrosis factor (TNF) receptor-associated factors (TRAFs) family which helps suppress programmed cell death and support cell survival. By doing so they maintain cellular homeostasis and balance between life and death signals in various physiological contexts.

Pathways

C-IAP1 and c-IAP2 are key components in the NF-kB and MAPK signaling pathways. These pathways are important for immune response inflammation and cell survival. c-IAPs interact with TNF receptor 1 (TNFR1) and receptor-interacting protein kinases (RIPKs) modulating downstream effects that activate NF-kB which transcribes genes involved in immune and survival responses. This connectivity highlights the involvement of c-IAP1 and c-IAP2 in pivotal signaling frameworks that coordinate cellular responses under stress and external stimuli.

C-IAP1 and c-IAP2 have significant roles in cancer and chronic inflammatory diseases. They are often overexpressed in various cancers including breast and lung cancer where they contribute to tumorigenesis by preventing cancer cell apoptosis and promoting cell survival. In the context of chronic inflammation these proteins maintain aberrant cell survival contributing to diseases such as rheumatoid arthritis. Connections with proteins like IκB kinase (IKK) highlight their impact on the perpetuation and progression of inflammatory conditions through their modulation of NF-kB signaling.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Multi-functional protein which regulates not only caspases and apoptosis, but also modulates inflammatory signaling and immunity, mitogenic kinase signaling, and cell proliferation, as well as cell invasion and metastasis. Acts as an E3 ubiquitin-protein ligase regulating NF-kappa-B signaling and regulates both canonical and non-canonical NF-kappa-B signaling by acting in opposite directions : acts as a positive regulator of the canonical pathway and suppresses constitutive activation of non-canonical NF-kappa-B signaling. The target proteins for its E3 ubiquitin-protein ligase activity include : RIPK1, RIPK2, RIPK3, RIPK4, CASP3, CASP7, CASP8, TRAF2, DIABLO/SMAC, MAP3K14/NIK, MAP3K5/ASK1, IKBKG/NEMO, IKBKE and MXD1/MAD1. Can also function as an E3 ubiquitin-protein ligase of the NEDD8 conjugation pathway, targeting effector caspases for neddylation and inactivation. Acts as an important regulator of innate immune signaling via regulation of Toll-like receptors (TLRs), Nodlike receptors (NLRs) and RIG-I like receptors (RLRs), collectively referred to as pattern recognition receptors (PRRs). Protects cells from spontaneous formation of the ripoptosome, a large multi-protein complex that has the capability to kill cancer cells in a caspase-dependent and caspase-independent manner. Suppresses ripoptosome formation by ubiquitinating RIPK1 and CASP8. Can stimulate the transcriptional activity of E2F1. Plays a role in the modulation of the cell cycle.
See full target information BIRC2
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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Evidence-based complementary and alternative medicine : eCAM 2021:1418616 PubMed34804173

2021

Scalp Acupuncture and Treadmill Training Inhibits Neuronal Apoptosis through Activating cIAP1 in Cerebral Ischemia Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Qiang Tang,Tao Ye,Runyu Liang,Yan Wang,Hongyu Li,Jiyao Zhang,Xingxing Yuan,Luwen Zhu

Cell death & disease 9:743 PubMed29970890

2018

Novel ADAM-17 inhibitor ZLDI-8 enhances the in vitro and in vivo chemotherapeutic effects of Sorafenib on hepatocellular carcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yingshi Zhang,Dandan Li,Qiyu Jiang,Shuang Cao,Huiwei Sun,Yantao Chai,Xiaojuan Li,Tianshu Ren,Ruichuang Yang,Fan Feng,Bo-An Li,Qingchun Zhao

Molecular medicine reports 18:1692-1703 PubMed29845197

2018

Tanshinone IIA regulates colorectal cancer apoptosis via attenuation of Parkin‑mediated mitophagy by suppressing AMPK/Skp2 pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Lili He,Kebo Gu

International journal of oncology 52:367-378 PubMed29207128

2017

NR4A1-induced increase in the sensitivity of a human gastric cancer line to TNFα-mediated apoptosis is associated with the inhibition of JNK/Parkin-dependent mitophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Hongzhu Yan,Feng Xiao,Jue Zou,Chengmin Qiu,Weiwei Sun,Minmin Gu,Li Zhang

Oncotarget 8:106833-106843 PubMed29290992

2017

Oridonin synergistically enhances JQ1-triggered apoptosis in hepatocellular cancer cells through mitochondrial pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hua-Peng Zhang,Gong-Quan Li,Wen-Zhi Guo,Guang-Hui Chen,Hong-Wei Tang,Bing Yan,Jie Li,Jia-Kai Zhang,Pei-Hao Wen,Zhi-Hui Wang,Jian-Feng Lv,Shui-Jun Zhang

Nutrition research and practice 11:445-451 PubMed29209454

2017

Kimchi attenuates fatty streak formation in the aorta of low-density lipoprotein receptor knockout mice via inhibition of endoplasmic reticulum stress and apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Minji Woo,Mijeong Kim,Jeong Sook Noh,Chan Hum Park,Yeong Ok Song

Nature communications 7:13336 PubMed27830706

2016

Biphasic regulation of chondrocytes by Rela through induction of anti-apoptotic and catabolic target genes.

Applications

Unspecified application

Species

Unspecified reactive species

Hiroshi Kobayashi,Song Ho Chang,Daisuke Mori,Shozo Itoh,Makoto Hirata,Yoko Hosaka,Yuki Taniguchi,Keita Okada,Yoshifumi Mori,Fumiko Yano,Ung-Il Chung,Haruhiko Akiyama,Hiroshi Kawaguchi,Sakae Tanaka,Taku Saito

Cell death and differentiation 17:439-51 PubMed19834489

2009

IGF-IR-dependent expression of Survivin is required for T-antigen-mediated protection from apoptosis and proliferation of neural progenitors.

Applications

IHC-P

Species

Mouse

E Gualco,K Urbanska,G Perez-Liz,T Sweet,F Peruzzi,K Reiss,L Del Valle

Cell death and differentiation 16:244-52 PubMed18846111

2008

hnRNP A1 regulates UV-induced NF-kappaB signalling through destabilization of cIAP1 mRNA.

Applications

WB

Species

Human

T T Zhao,T E Graber,L E Jordan,M Cloutier,S M Lewis,I Goulet,J Côté,M Holcik
View all publications
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