Anti-c-Jun antibody [3/Jun] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Jun antibody [3/Jun] - BSA and Azide free (AB280094)

This data was developed using ab280089 the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human esophagus tissue labeling c-Jun with ab280089 at 1/5000 (0.196 μg/ml) dilution followed by a ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Nuclear staining on human esophagus.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

Heat mediated antigen retrieval using ab93678 (citrate buffer, pH 6.0).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-c-Jun antibody [3/Jun] - BSA and Azide free (AB280094)

This data was developed using ab280089 the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MDA-MB-231 cells labelling c-Jun with ab280089 at 1/50 (19.56 μg/ml) dilution, followed by ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green).

Confocal image showing nuclear staining in MDA-MB-231 cells.

Negative control : MCF7 (PMID : 24830720).

ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

Negative control 1 : ab280089 at a 1/50 dilution followed by ab150080 at a 1/500 dilution.

Negative control 2 : ab179513 at a 1/200 dilution followed by ab150113 at a 1/1000 dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Jun antibody [3/Jun] - BSA and Azide free (AB280094)

This data was developed using ab280089 the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling c-Jun with ab280089 at 1/5000 (0.196 μg/ml) dilution followed by a ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Scattered nuclear staining on human colon.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

Heat mediated antigen retrieval using ab93678 (citrate buffer, pH 6.0).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Jun antibody [3/Jun] - BSA and Azide free (AB280094)

This data was developed using ab280089 the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling c-Jun with ab280089 at 1/5000 (0.196 μg/ml) dilution followed by a ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Nuclear staining on rat cerebrum.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

Heat mediated antigen retrieval using ab93678 (citrate buffer, pH 6.0).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Jun antibody [3/Jun] - BSA and Azide free (AB280094)

This data was developed using ab280089 the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling c-Jun with ab280089 at 1/5000 (0.196 μg/ml) dilution followed by a ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Nuclear staining on mouse cerebrum.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

Heat mediated antigen retrieval using ab93678 (citrate buffer, pH 6.0).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Jun antibody [3/Jun] - BSA and Azide free (AB280094)

This data was developed using ab280089 the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue labeling c-Jun with ab280089 at 1/5000 (0.196 μg/ml) dilution followed by a ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Nuclear staining on the dentate gyrus of rat hippocampus.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

Heat mediated antigen retrieval using ab93678 (citrate buffer, pH 6.0).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Jun antibody [3/Jun] - BSA and Azide free (AB280094)

This data was developed using ab280089 the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue labeling c-Jun with ab280089 at 1/5000 (0.196 μg/ml) dilution followed by a ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Nuclear staining on the dentate gyrus of mouse hippocampus.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

Heat mediated antigen retrieval using ab93678 (citrate buffer, pH 6.0).

• WB

Supplier Data

Western blot - Anti-c-Jun antibody [3/Jun] - BSA and Azide free (AB280094)

This data was developed using ab280089 the same antibody clone in a different buffer formulation.

Blocking/Dilution buffer : 5% NFDM/TBST.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 20976196).

Negative control : T-47D (PMID : 24830720).

Exposure time : lane 1 : 3 minutes
lane 2, 3 : 15 seconds
lane 4 : 37 seconds

All lanes:

Western blot - Anti-c-Jun antibody [3/Jun] (<a href='/en-us/products/primary-antibodies/c-jun-antibody-3-jun-ab280089'>ab280089</a>) at 1/1000 dilution

Lane 1:

HEK-293 (human embryonic kidney epithelial cell), whole cell lysate at 20 µg

Lane 2:

T-47D (human ductal breast epithelial tumor epithelial cell), whole cell lysate at 20 µg

Lane 3:

MDA-MB-231 (human breast adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Predicted band size: 35 kDa

false

• WB

Supplier Data

Western blot - Anti-c-Jun antibody [3/Jun] - BSA and Azide free (AB280094)

This data was developed using ab280089 the same antibody clone in a different buffer formulation.

Lysates were made freshly and used in WB immediately to minimize protein degradation.

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure times : lane 1 : 3 mins;

lane 2, 3 : 15 secs;

lane 4 : 37 secs.

All lanes:

Western blot - Anti-c-Jun antibody [3/Jun] (<a href='/en-us/products/primary-antibodies/c-jun-antibody-3-jun-ab280089'>ab280089</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell ), whole cell lysate at 20 µg

Lane 2:

HEK-293 (human embryonic kidney epithelial cell), whole cell lysate at 20 µg

Lane 3:

NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 20 µg

Lane 4:

PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Predicted band size: 35 kDa

false