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AB31419

Anti-c-Jun antibody

5

(13 Reviews)

|

(95 Publications)

Rabbit Polyclonal c-Jun antibody. Suitable for IP, ChIP, Flow Cyt, ELISA, WB, IHC-P, ICC/IF and reacts with Mouse, Rat, African green monkey, Human, Vervet monkey samples. Cited in 95 publications. Immunogen corresponding to Synthetic Peptide within Human JUN aa 200-300.

View Alternative Names

Transcription factor Jun, Activator protein 1, Proto-oncogene c-Jun, Transcription factor AP-1 subunit Jun, V-jun avian sarcoma virus 17 oncogene homolog, p39, AP1, JUN

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-c-Jun antibody (AB31419)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-c-Jun antibody (AB31419)

ICC/IF image of HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling c-Jun (green) with ab31419 at 1 μg/ml. The cells were fixed in 4% PFA (10 minutes) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1 hour to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with ab31419 at 1 μg/ml overnight at +4 °C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) ab150077 used at a 1/1000 dilution for 1 hour. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1 hour. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43 μM.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Jun antibody (AB31419)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Jun antibody (AB31419)

Paraffin-embedded human breast carcinoma tissue stained for c-Jun with ab31419 at a 1/50 dilution in immunohistochemical analysis.

Left panel : Untreated.

Right panel : Pre-incubated with synthesized peptide.

Western blot - Anti-c-Jun antibody (AB31419)
  • WB

Unknown

Western blot - Anti-c-Jun antibody (AB31419)

All lanes:

Western blot - Anti-c-Jun antibody (ab31419) at 1/500 dilution

Lane 1:

Extracts of Hela (Human epithelial cell line from cervix adenocarcinoma) cells

Lane 2:

Extracts of Hela (Human epithelial cell line from cervix adenocarcinoma) cells with immunizing peptide

Predicted band size: 35 kDa

Observed band size: 43 kDa

false

Western blot - Anti-c-Jun antibody (AB31419)
  • WB

Unknown

Western blot - Anti-c-Jun antibody (AB31419)

All lanes:

Western blot - Anti-c-Jun antibody (ab31419) at 1/500 dilution

All lanes:

Recombinant Human c-Jun protein (<a href='/en-us/products/unavailable/recombinant-human-c-jun-protein-ab54318'>ab54318</a>) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 35 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse, African green monkey, Rat

Applications

ELISA, WB, ICC/IF, ChIP, IP, Flow Cyt, IHC-P

applications

Immunogen

Synthetic Peptide within Human JUN aa 200-300. The exact immunogen used to generate this antibody is proprietary information.

P05412

Specificity

Antibody detects endogenous levels of c-Jun protein around Serine 243.

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Complementary Products

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Anti-c-Fos antibody [EPR883(2)]

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Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage buffer
pH: 7 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein c-Jun is an important component of the AP-1 transcription factor complex and is often referred to by alternative names such as c-jun. It has a molecular weight of about 39 kDa. Scientists have found c-jun expressed in a wide range of tissues indicating its diverse roles in cellular functions. This protein plays a critical mechanical role by binding specific DNA sequences to regulate the transcription of various genes involved in cell proliferation and apoptosis.
Biological function summary

C-Jun impacts cellular activities by being a significant part of the AP-1 transcription factor complex interacting with other proteins such as Fos. This interaction allows c-Jun to modulate gene expression in response to cellular stimuli. In particular c-Jun influences cell cycle progression and differentiation. Therefore its activity is necessary for physiological and pathological processes. As c-jun's function remains tightly regulated any changes can have wide-ranging effects.

Pathways

C-Jun significantly influences the MAPK/ERK and JNK pathways. These pathways play important roles in cellular responses to stress growth factors and cytokines. Within these pathways c-Jun interacts with proteins such as JNK which phosphorylates c-Jun and enhances its activity. These interactions allow c-Jun to regulate target genes involved in important cellular processes without much delay. Understanding c-Jun's function in these pathways helps elucidate how cells control division survival and apoptosis.

C-Jun's dysregulation connects to various conditions including cancer and neurodegenerative diseases. Abnormal c-Jun activity can lead to oncogenesis by promoting unrestrained cellular proliferation. Additionally c-Jun involvement in disorders like Alzheimer's disease stems from its role in apoptotic pathways which may cause neuronal death. Accompanying proteins like JNK are also implicated in these conditions making the regulation and expression of c-Jun a focus for therapeutic research.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription factor that recognizes and binds to the AP-1 consensus motif 5'-TGA[GC]TCA-3' (PubMed : 10995748, PubMed : 22083952). Heterodimerizes with proteins of the FOS family to form an AP-1 transcription complex, thereby enhancing its DNA binding activity to the AP-1 consensus sequence 5'-TGA[GC]TCA-3' and enhancing its transcriptional activity (By similarity). Together with FOSB, plays a role in activation-induced cell death of T cells by binding to the AP-1 promoter site of FASLG/CD95L, and inducing its transcription in response to activation of the TCR/CD3 signaling pathway (PubMed : 12618758). Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation (PubMed : 17210646). Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells (PubMed : 24623306). Binds to the USP28 promoter in colorectal cancer (CRC) cells (PubMed : 24623306).. (Microbial infection) Upon Epstein-Barr virus (EBV) infection, binds to viral BZLF1 Z promoter and activates viral BZLF1 expression.
See full target information JUN
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Publications (95)

Recent publications for all applications. Explore the full list and refine your search

Cell death discovery 11:170 PubMed40222967

2025

Acetylcholine muscarinic M2 receptor maintains human Schwann-like adipose-derived phenotype in the absence of differentiating medium.

Applications

Unspecified application

Species

Unspecified reactive species

Roberta Piovesana,Alessandro Faroni,Ada Maria Tata,Adam J Reid

JCI insight 9: PubMed38194296

2024

BDNF and cAMP are neuroprotective in a porcine model of traumatic optic neuropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Kathleen Heng,Brent K Young,BaoXiang Li,Ashley D Nies,Xin Xia,Runxia R Wen,Roopa Dalal,Gregory T Bramblett,Andrew W Holt,Jeffery M Cleland,Jason N Harris,Albert Y Wu,Jeffrey L Goldberg

iScience 26:108555 PubMed38125029

2023

Quantitative proteomics and RNA-sequencing of mouse liver endothelial cells identify novel regulators of BMP6 by iron.

Applications

Unspecified application

Species

Unspecified reactive species

Allison L Fisher,Chia-Yu Wang,Yang Xu,Sydney Phillips,Joao A Paulo,Beata Małachowska,Xia Xiao,Wojciech Fendler,Joseph D Mancias,Jodie L Babitt

Cell & bioscience 13:191 PubMed37838693

2023

c-Jun as a one-way valve at the naive to primed interface.

Applications

Unspecified application

Species

Unspecified reactive species

Dongwei Li,Ling Luo,Lin Guo,Chuman Wu,Ran Zhang,Yuling Peng,Menghua Wu,Junqi Kuang,Yan Li,Yudan Zhang,Jun Xie,Wenxiu Xie,Rui Mao,Gang Ma,Xiuling Fu,Jiekai Chen,Andrew P Hutchins,Duanqing Pei

Genes and environment : the official journal of the Japanese Environmental Mutagen Society 44:23 PubMed36163195

2022

MicroRNA miR-627-5p restrains pulmonary artery smooth muscle cell dysfunction by targeting MAP 2 K4 and PI3K/AKT signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Li,Xiaoqin Tan,Yuexia Huang,Jun Cui,Fan Chen,Ying Xiong

Cell reports 40:111315 PubMed36070691

2022

Runx2 regulates chromatin accessibility to direct the osteoblast program at neonatal stages.

Applications

Unspecified application

Species

Unspecified reactive species

Hironori Hojo,Taku Saito,Xinjun He,Qiuyu Guo,Shoko Onodera,Toshifumi Azuma,Michinori Koebis,Kazuki Nakao,Atsu Aiba,Masahide Seki,Yutaka Suzuki,Hiroyuki Okada,Sakae Tanaka,Ung-Il Chung,Andrew P McMahon,Shinsuke Ohba

Frontiers in endocrinology 13:946492 PubMed35992146

2022

identification and verification of ferroptosis-related genes in type 2 diabetic islets.

Applications

Unspecified application

Species

Unspecified reactive species

Meiqi Yin,Liang Zhou,Yanan Ji,Rongxin Lu,Wei Ji,Guorong Jiang,Jin Ma,Xiudao Song

Journal of immunology research 2022:8440422 PubMed35942209

2022

Transmembrane Protein ANTXR1 Regulates -Globin Expression by Targeting the Wnt/-Catenin Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Tingting Jin,Zhaojun Zhang,Yuanyuan Han,Di Li,Juan Liu,Minmin Jiang,Junwei Zhu,Ryo Kurita,Yukio Nakamura,Fangfang Hu,Yongjie Xu,Xiangdong Fang,Shengwen Huang,Zhaolin Sun

Stem cells (Dayton, Ohio) 40:818-830 PubMed35728620

2022

Epigenetic Regulation of NGF-Mediated Osteogenic Differentiation in Human Dental Mesenchymal Stem Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenqing Liu,Jin Sook Suh,Peng Deng,Olga Bezouglaia,Megan Do,Mojan Mirnia,Zhong-Kai Cui,Min Lee,Tara Aghaloo,Cun-Yu Wang,Christine Hong

Translational cancer research 11:813-822 PubMed35571660

2022

Inhibitory effect of lovastatin on human lung cancer cell proliferation by regulating the ERK1/2 and COX-2 pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Sha Liu,Ping Yang,Mingkung Wang,Shuang Zhang,Jie Wang,Tao Pan,Ping Zhou
View all publications
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