Rabbit Polyclonal c-Jun antibody. Suitable for IP, ChIP, Flow Cyt, ELISA, WB, IHC-P, ICC/IF and reacts with Mouse, Rat, African green monkey, Human, Vervet monkey samples. Cited in 89 publications. Immunogen corresponding to Synthetic Peptide within Human JUN aa 200-300.
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- iScience 26:1085552023Quantitative proteomics and RNA-sequencing of mouse liver endothelial cells identify novel regulators of BMP6 by iron.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAllison L Fisher,Chia-Yu Wang,Yang Xu,Sydney Phillips,Joao A Paulo,Beata Małachowska,Xia Xiao,Wojciech Fendler,Joseph D Mancias,Jodie L BabittPubMed 38125029
- Cell & bioscience 13:1912023c-Jun as a one-way valve at the naive to primed interface.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDongwei Li,Ling Luo,Lin Guo,Chuman Wu,Ran Zhang,Yuling Peng,Menghua Wu,Junqi Kuang,Yan Li,Yudan Zhang,Jun Xie,Wenxiu Xie,Rui Mao,Gang Ma,Xiuling Fu,Jiekai Chen,Andrew P Hutchins,Duanqing PeiPubMed 37838693
- Genes and environment : the official journal of the Japanese Environmental Mutagen Society 44:232022MicroRNA miR-627-5p restrains pulmonary artery smooth muscle cell dysfunction by targeting MAP 2 K4 and PI3K/AKT signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTing Li,Xiaoqin Tan,Yuexia Huang,Jun Cui,Fan Chen,Ying XiongPubMed 36163195
- Frontiers in endocrinology 13:9464922022identification and verification of ferroptosis-related genes in type 2 diabetic islets.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMeiqi Yin,Liang Zhou,Yanan Ji,Rongxin Lu,Wei Ji,Guorong Jiang,Jin Ma,Xiudao SongPubMed 35992146
- Journal of immunology research 2022:84404222022Transmembrane Protein ANTXR1 Regulates -Globin Expression by Targeting the Wnt/-Catenin Signaling Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTingting Jin,Zhaojun Zhang,Yuanyuan Han,Di Li,Juan Liu,Minmin Jiang,Junwei Zhu,Ryo Kurita,Yukio Nakamura,Fangfang Hu,Yongjie Xu,Xiangdong Fang,Shengwen Huang,Zhaolin SunPubMed 35942209
- Stem cells (Dayton, Ohio) 40:818-8302022Epigenetic Regulation of NGF-Mediated Osteogenic Differentiation in Human Dental Mesenchymal Stem Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhenqing Liu,Jin Sook Suh,Peng Deng,Olga Bezouglaia,Megan Do,Mojan Mirnia,Zhong-Kai Cui,Min Lee,Tara Aghaloo,Cun-Yu Wang,Christine HongPubMed 35728620
- Translational cancer research 11:813-8222022Inhibitory effect of lovastatin on human lung cancer cell proliferation by regulating the ERK1/2 and COX-2 pathways.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSha Liu,Ping Yang,Mingkung Wang,Shuang Zhang,Jie Wang,Tao Pan,Ping ZhouPubMed 35571660
- iScience 25:1043252022c-JUN inhibits mTORC2 and glucose uptake to promote self-renewal and obesity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRaphael Serna,Ambika Ramrakhiani,Juan Carlos Hernandez,Chia-Lin Chen,Chad Nakagawa,Tatsuya Machida,Ratna B Ray,Xiaohang Zhan,Stanley M Tahara,Keigo MachidaPubMed 35601917
- Physiological research 71:259-2732022Protection of toll-like receptor 9 against lipopolysaccharide-induced inflammation and oxidative stress of pulmonary epithelial cells via MyD88-mediated pathways.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhenhong Qi,Zhijie He,Jiaxin Chen,Mingyuan Ma,Menghua Deng,Yunhai Zhang,Tianwei MaPubMed 35275698
- Heliyon 8:e088902022Involvement of activator protein-1 family members in β-catenin and p300 association on the genome of PANC-1 cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTomomitsu Doi,Hironori Hojo,Shinsuke Ohba,Kunie Obayashi,Motoyoshi Endo,Toshimasa Ishizaki,Akira Katoh,Hiroyuki KoujiPubMed 35198763
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Synthetic Peptide within Human JUN aa 200-300. The exact immunogen used to generate this antibody is proprietary information. Database link P05412
Consider this alternative
Reactivity data
Select an application| IP | ChIP | Flow Cyt | ELISA | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|---|---|---|
| Human | Expected | Expected | Expected | Expected | Tested | Tested | Tested |
| Mouse | Expected | Expected | Expected | Expected | Predicted | Predicted | Predicted |
| Rat | Expected | Expected | Expected | Expected | Predicted | Predicted | Predicted |
| African green monkey | Expected | Expected | Predicted | Predicted | Predicted | Predicted | Predicted |
| Chicken | Predicted | Predicted | Predicted | Predicted | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted | Predicted | Predicted | Predicted | Predicted |
| Pig | Predicted | Predicted | Predicted | Predicted | Predicted | Predicted | Predicted |
| Vervet monkey | Not recommended | Not recommended | Expected | Expected | Not recommended | Not recommended | Not recommended |
IP
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, African green monkey, Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Cow, Pig | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Vervet monkey | Dilution info - | Notes - |
ChIP
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, African green monkey, Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Cow, Pig | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Vervet monkey | Dilution info - | Notes - |
Flow Cyt
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
Species Rat | Dilution info - | Notes ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
Species Vervet monkey | Dilution info - | Notes ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
Species Human | Dilution info - | Notes ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Cow, Pig, African green monkey | Dilution info - | Notes - |
ELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Vervet monkey, Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Cow, Pig, African green monkey | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/1000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Cow, Pig, Mouse, African green monkey, Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Vervet monkey | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 - 1/100 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Cow, Pig, Mouse, African green monkey, Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Vervet monkey | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Cow, Pig, Mouse, African green monkey, Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Vervet monkey | Dilution info - | Notes - |
Associated Products
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5 products for Alternative Product
Target data
Function
Transcription factor that recognizes and binds to the AP-1 consensus motif 5'-TGA[GC]TCA-3' (PubMed:10995748, PubMed:22083952). Heterodimerizes with proteins of the FOS family to form an AP-1 transcription complex, thereby enhancing its DNA binding activity to the AP-1 consensus sequence 5'-TGA[GC]TCA-3' and enhancing its transcriptional activity (By similarity). Together with FOSB, plays a role in activation-induced cell death of T cells by binding to the AP-1 promoter site of FASLG/CD95L, and inducing its transcription in response to activation of the TCR/CD3 signaling pathway (PubMed:12618758). Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation (PubMed:17210646). Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells (PubMed:24623306). Binds to the USP28 promoter in colorectal cancer (CRC) cells (PubMed:24623306).(Microbial infection) Upon Epstein-Barr virus (EBV) infection, binds to viral BZLF1 Z promoter and activates viral BZLF1 expression.
Alternative names
Transcription factor Jun, Activator protein 1, Proto-oncogene c-Jun, Transcription factor AP-1 subunit Jun, V-jun avian sarcoma virus 17 oncogene homolog, p39, AP1, JUN
Recommended products
Rabbit Polyclonal c-Jun antibody. Suitable for IP, ChIP, Flow Cyt, ELISA, WB, IHC-P, ICC/IF and reacts with Mouse, Rat, African green monkey, Human, Vervet monkey samples. Cited in 89 publications. Immunogen corresponding to Synthetic Peptide within Human JUN aa 200-300.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Synthetic Peptide within Human JUN aa 200-300. The exact immunogen used to generate this antibody is proprietary information. Database link P05412
- Purification technique
Affinity purification Immunogen
- Specificity
Antibody detects endogenous levels of c-Jun protein around Serine 243.
- Concentration
- Purification notes
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein c-Jun is an important component of the AP-1 transcription factor complex and is often referred to by alternative names such as c-jun. It has a molecular weight of about 39 kDa. Scientists have found c-jun expressed in a wide range of tissues indicating its diverse roles in cellular functions. This protein plays a critical mechanical role by binding specific DNA sequences to regulate the transcription of various genes involved in cell proliferation and apoptosis.
- Biological function summary
C-Jun impacts cellular activities by being a significant part of the AP-1 transcription factor complex interacting with other proteins such as Fos. This interaction allows c-Jun to modulate gene expression in response to cellular stimuli. In particular c-Jun influences cell cycle progression and differentiation. Therefore its activity is necessary for physiological and pathological processes. As c-jun's function remains tightly regulated any changes can have wide-ranging effects.
- Pathways
C-Jun significantly influences the MAPK/ERK and JNK pathways. These pathways play important roles in cellular responses to stress growth factors and cytokines. Within these pathways c-Jun interacts with proteins such as JNK which phosphorylates c-Jun and enhances its activity. These interactions allow c-Jun to regulate target genes involved in important cellular processes without much delay. Understanding c-Jun's function in these pathways helps elucidate how cells control division survival and apoptosis.
- Associated diseases and disorders
C-Jun's dysregulation connects to various conditions including cancer and neurodegenerative diseases. Abnormal c-Jun activity can lead to oncogenesis by promoting unrestrained cellular proliferation. Additionally c-Jun involvement in disorders like Alzheimer's disease stems from its role in apoptotic pathways which may cause neuronal death. Accompanying proteins like JNK are also implicated in these conditions making the regulation and expression of c-Jun a focus for therapeutic research.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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4 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Jun antibody (ab31419)
Paraffin-embedded human breast carcinoma tissue stained for c-Jun with ab31419 at a 1/50 dilution in immunohistochemical analysis.
Left panel: Untreated.
Right panel: Pre-incubated with synthesized peptide.
Immunocytochemistry/ Immunofluorescence - Anti-c-Jun antibody (ab31419)
ICC/IF image of HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling c-Jun (green) with ab31419 at 1 μg/ml. The cells were fixed in 4% PFA (10 minutes) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1 hour to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with ab31419 at 1 μg/ml overnight at +4 °C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 used at a 1/1000 dilution for 1 hour. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1 hour. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43 μM.
Western blot - Anti-c-Jun antibody (ab31419)
All lanes: Western blot - Anti-c-Jun antibody (ab31419) at 1/500 dilution
All lanes: Extracts of Hela (Human epithelial cell line from cervix adenocarcinoma) cells
Predicted band size: 35 kDa
Observed band size: 43 kDa
Western blot - Anti-c-Jun antibody (ab31419)
All lanes: Western blot - Anti-c-Jun antibody (ab31419) at 1/500 dilution
All lanes: Recombinant Human c-Jun protein (ab54318) at 0.01 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 35 kDa
Exposure time: 30s
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