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Rabbit Recombinant Monoclonal c-Jun phospho S63 antibody. Carrier free. Suitable for ELISA, Dot, WB, ICC/IF, IHC-P and reacts with Synthetic peptide, Human, Mouse samples. Cited in 38 publications.

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Images

Western blot - Anti-c-Jun (phospho S63) antibody [Y172] - BSA and Azide free (AB227533), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Jun (phospho S63) antibody [Y172] - BSA and Azide free (AB227533), expandable thumbnail
  • Western blot - Anti-c-Jun (phospho S63) antibody [Y172] - BSA and Azide free (AB227533), expandable thumbnail
  • Western blot - Anti-c-Jun (phospho S63) antibody [Y172] - BSA and Azide free (AB227533), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-c-Jun (phospho S63) antibody [Y172] - BSA and Azide free (AB227533), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytELISADotWBICC/IFIHC-P
Human
Not recommended
Not recommended
Expected
Tested
Expected
Tested
Tested
Mouse
Not recommended
Not recommended
Expected
Expected
Tested
Expected
Expected
Rat
Not recommended
Not recommended
Predicted
Predicted
Predicted
Predicted
Predicted
Cow
Not recommended
Not recommended
Predicted
Predicted
Predicted
Predicted
Predicted
Synthetic peptide
Not recommended
Not recommended
Tested
Not recommended
Not recommended
Not recommended
Not recommended

Not recommended
Not recommended

Species

Mouse

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Human

Dilution info

-

Notes

-

Species

Cow

Dilution info

-

Notes

-

Species

Rat

Dilution info

-

Notes

-

Species

Synthetic peptide

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Human, Cow, Rat, Synthetic peptide

Dilution info

-

Notes

-

Tested
Tested

Species

Synthetic peptide

Dilution info

-

Notes

-

Expected
Expected

Species

Human, Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Cow, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Cow, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Synthetic peptide

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

-

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Cow, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Synthetic peptide

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Cow, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Synthetic peptide

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse.

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Cow, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Synthetic peptide

Dilution info

-

Notes

-

Associated Products

Select an associated product type

15 products for Alternative Product

2 products for Alternative Version

Target data

Function

Transcription factor that recognizes and binds to the AP-1 consensus motif 5'-TGA[GC]TCA-3' (PubMed:10995748, PubMed:22083952). Heterodimerizes with proteins of the FOS family to form an AP-1 transcription complex, thereby enhancing its DNA binding activity to the AP-1 consensus sequence 5'-TGA[GC]TCA-3' and enhancing its transcriptional activity (By similarity). Together with FOSB, plays a role in activation-induced cell death of T cells by binding to the AP-1 promoter site of FASLG/CD95L, and inducing its transcription in response to activation of the TCR/CD3 signaling pathway (PubMed:12618758). Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation (PubMed:17210646). Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells (PubMed:24623306). Binds to the USP28 promoter in colorectal cancer (CRC) cells (PubMed:24623306).(Microbial infection) Upon Epstein-Barr virus (EBV) infection, binds to viral BZLF1 Z promoter and activates viral BZLF1 expression.

Alternative names

Recommended products

Rabbit Recombinant Monoclonal c-Jun phospho S63 antibody. Carrier free. Suitable for ELISA, Dot, WB, ICC/IF, IHC-P and reacts with Synthetic peptide, Human, Mouse samples. Cited in 38 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

Y172

Purification technique

Affinity purification Protein A

Specificity

The antibody only detects c-Jun phosphorylated on Serine 63 when tested in WB and ICC using specific phospho-treatments. However, in DotBlot and ELISA assays we detected some cross-reactivity with the non-phospho peptide as well. Please refer to the images on the datasheet. The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab227533 is the carrier-free version of Anti-c-Jun (phospho S63) antibody [Y172] ab32385.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The protein c-Jun is an important component of the AP-1 transcription factor complex and is often referred to by alternative names such as c-jun. It has a molecular weight of about 39 kDa. Scientists have found c-jun expressed in a wide range of tissues indicating its diverse roles in cellular functions. This protein plays a critical mechanical role by binding specific DNA sequences to regulate the transcription of various genes involved in cell proliferation and apoptosis.

Biological function summary

C-Jun impacts cellular activities by being a significant part of the AP-1 transcription factor complex interacting with other proteins such as Fos. This interaction allows c-Jun to modulate gene expression in response to cellular stimuli. In particular c-Jun influences cell cycle progression and differentiation. Therefore its activity is necessary for physiological and pathological processes. As c-jun's function remains tightly regulated any changes can have wide-ranging effects.

Pathways

C-Jun significantly influences the MAPK/ERK and JNK pathways. These pathways play important roles in cellular responses to stress growth factors and cytokines. Within these pathways c-Jun interacts with proteins such as JNK which phosphorylates c-Jun and enhances its activity. These interactions allow c-Jun to regulate target genes involved in important cellular processes without much delay. Understanding c-Jun's function in these pathways helps elucidate how cells control division survival and apoptosis.

Associated diseases and disorders

C-Jun's dysregulation connects to various conditions including cancer and neurodegenerative diseases. Abnormal c-Jun activity can lead to oncogenesis by promoting unrestrained cellular proliferation. Additionally c-Jun involvement in disorders like Alzheimer's disease stems from its role in apoptotic pathways which may cause neuronal death. Accompanying proteins like JNK are also implicated in these conditions making the regulation and expression of c-Jun a focus for therapeutic research.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Western blot - Anti-c-Jun (phospho S63) antibody [Y172] - BSA and Azide free (ab227533), expandable thumbnail

    Western blot - Anti-c-Jun (phospho S63) antibody [Y172] - BSA and Azide free (ab227533)

    Blocking and diluting buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-c-Jun (phospho S63) antibody [Y172] ab32385).

    All lanes: Western blot - Anti-c-Jun (phospho S63) antibody [Y172] (Anti-c-Jun (phospho S63) antibody [Y172] ab32385) at 0.1 µg/mL

    Lane 1: NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates at 15 µg

    Lane 2: NIH/3T3 (Mouse embryonic fibroblast) treated with 250 ng/ml anisomycin for 30 minutes whole cell lysates at 15 µg

    Lane 3: NIH/3T3 (Mouse embryonic fibroblast) treated with 250 ng/ml anisomycin for 30 minutes whole cell lysates. Then the membrane was incubated with phosphatase at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 35 kDa

    Blocking and diluting buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Jun (phospho S63) antibody [Y172] - BSA and Azide free (ab227533), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Jun (phospho S63) antibody [Y172] - BSA and Azide free (ab227533)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast tissue sections labeling c-Jun with Purified Anti-c-Jun (phospho S63) antibody [Y172] ab32385 at 1:250 dilution (0.46 µg/ml). Heat mediated antigen retrieval was performed using using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0)ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylinwas used as a counterstainThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-c-Jun (phospho S63) antibody [Y172] ab32385).

  • Western blot - Anti-c-Jun (phospho S63) antibody [Y172] - BSA and Azide free (ab227533), expandable thumbnail

    Western blot - Anti-c-Jun (phospho S63) antibody [Y172] - BSA and Azide free (ab227533)

    Blocking and diluting buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-c-Jun (phospho S63) antibody [Y172] (Anti-c-Jun (phospho S63) antibody [Y172] ab32385) at 0.1 µg/mL

    Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg

    Lane 2: HeLa (Human cervix adenocarcinoma epithelial cell) treated with 1 ug/ml anisomycin for 15 minutes whole cell lysates at 15 µg

    Lane 3: HeLa (Human cervix adenocarcinoma epithelial cell) treated with 1 ug/ml anisomycin for 15 minutes whole cell lysates 15ug. Then the membrane was incubated with phosphatase. at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 35 kDa

    Blocking and diluting buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-c-Jun (phospho S63) antibody [Y172] ab32385).

  • Western blot - Anti-c-Jun (phospho S63) antibody [Y172] - BSA and Azide free (ab227533), expandable thumbnail

    Western blot - Anti-c-Jun (phospho S63) antibody [Y172] - BSA and Azide free (ab227533)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-c-Jun (phospho S63) antibody [Y172] ab32385).

    Lane 1: Western blot - Anti-c-Jun (phospho S63) antibody [Y172] (Anti-c-Jun (phospho S63) antibody [Y172] ab32385) at 1/1000 dilution

    Lanes 2 - 3: Western blot - Anti-c-Jun (phospho S63) antibody [Y172] - BSA and Azide free (ab227533) at 1/1000 dilution

    Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg

    Lane 2: HeLa (Human cervix adenocarcinoma epithelial cell) treated with 1ug/mL anisomycin for 15 minutes whole cell lysates at 15 µg

    Lane 3: HeLa (Human cervix adenocarcinoma epithelial cell) treated with 1ug/ml anisomycin for 15 minutes whole cell lysates. Then the membrane was incubated with phosphatase. at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 35 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-c-Jun (phospho S63) antibody [Y172] - BSA and Azide free (ab227533), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-c-Jun (phospho S63) antibody [Y172] - BSA and Azide free (ab227533)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A431 (Human epidermoid carcinoma cell line) cells labeling c-Jun (phospho S63) with Anti-c-Jun (phospho S63) antibody [Y172] ab32385 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing the expression was increased after treatment with anisomycin (1 µg/ml for 15 minutes), then decreased after treatment with the Lambda Protein Phosphatase treatment 31? for 2 hours. The nuclear counter stain is DAPI (blue).
    Counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at a 1/200 dilution (red).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-c-Jun (phospho S63) antibody [Y172] ab32385).

  • ELISA - Anti-c-Jun (phospho S63) antibody [Y172] - BSA and Azide free (ab227533), expandable thumbnail

    ELISA - Anti-c-Jun (phospho S63) antibody [Y172] - BSA and Azide free (ab227533)

    Antigen pS63:c-Jun (phospho S63); NP:c-Jun non-phospho. Antigen concentration 0.01~1 ng/ml.

    Primary antibody concentration range 0~1000 ng/ml.

    Secondary antibody is an Alkaline Phosphatase-conjugated Goat Anti-Rabbit IgG(H+L) used at a 1:2500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-c-Jun (phospho S63) antibody [Y172] ab32385).

  • Dot Blot - Anti-c-Jun (phospho S63) antibody [Y172] - BSA and Azide free (ab227533), expandable thumbnail

    Dot Blot - Anti-c-Jun (phospho S63) antibody [Y172] - BSA and Azide free (ab227533)

    Unpurified Anti-c-Jun (phospho S63) antibody [Y172] ab32385 used at a 1:1000 dilution.
    Secondary antibody is Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) used at a 1:100,000 dilution. Blocking/Diluting buffer and concentration: 5% NFDM/TBST.
    Lane 1: Human c-Jun (pS63) phospho peptide.
    Lane 2: Human c-Jun non-phospho peptide.
    Exposure time 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-c-Jun (phospho S63) antibody [Y172] ab32385).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Jun (phospho S63) antibody [Y172] - BSA and Azide free (ab227533), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Jun (phospho S63) antibody [Y172] - BSA and Azide free (ab227533)

    This IHC data was generated using the same anti-c Jun antibody clone, Y172, in a different buffer formulation (cat# Anti-c-Jun (phospho S63) antibody [Y172] ab32385).

    Anti-c-Jun (phospho S63) antibody [Y172] ab32385, at a 1/50 dilution, staining c-Jun in paraffin embedded human breast carcinoma tissue by Immunohistochemistry.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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