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AB326669

Anti-c-Jun (phospho s73) + JunD (phospho s100) antibody [SR1627]

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Rabbit Recombinant Monoclonal c-Jun phospho S73 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse samples.

View Alternative Names

Transcription factor Jun, Activator protein 1, Proto-oncogene c-Jun, Transcription factor AP-1 subunit Jun, V-jun avian sarcoma virus 17 oncogene homolog, p39, AP1, JUN

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Jun (phospho s73) + JunD (phospho s100) antibody [SR1627] (AB326669)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Jun (phospho s73) + JunD (phospho s100) antibody [SR1627] (AB326669)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for c-Jun (phospho s73) + JunD (phospho s100)using antibody at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-c-Jun (phospho s73) + JunD (phospho s100) antibody [SR1627] (AB326669)
  • WB

Supplier Data

Western blot - Anti-c-Jun (phospho s73) + JunD (phospho s100) antibody [SR1627] (AB326669)

All lanes : Primary antibody at 1/1000 dilution

Lane 1 : HeLa cell lysate
Lane 2 : HeLa treated with 5ug/mL Anisomycin for 30 minutes cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size : 36 kDa
Observed band size : 38-40 kDa

Exposure time : 4 seconds

All lanes:

Western blot - Anti-c-Jun (phospho s73) + JunD (phospho s100) antibody [SR1627] (ab326669) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

HeLa treated with 5ug/mL Anisomycin for 30 minutes cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 36 kDa

Observed band size: 38-40 kDa

false

Exposure time: 4s

Western blot - Anti-c-Jun (phospho s73) + JunD (phospho s100) antibody [SR1627] (AB326669)
  • WB

Supplier Data

Western blot - Anti-c-Jun (phospho s73) + JunD (phospho s100) antibody [SR1627] (AB326669)

All lanes : Primary antibody at 1/1000 dilution

Lane 1 : NIH/3T3 cell lysate
Lane 2 : NIH/3T3 treated with 25ug/mL Anisomycin for 30 minutes cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size : 36 kDa
Observed band size : 38-40 kDa

Exposure time : 5 seconds

All lanes:

Western blot - Anti-c-Jun (phospho s73) + JunD (phospho s100) antibody [SR1627] (ab326669) at 1/1000 dilution

Lane 1:

NIH/3T3 cell lysate at 20 µg

Lane 2:

NIH/3T3 treated with 25ug/mL Anisomycin for 30 minutes cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 36 kDa

Observed band size: 38-40 kDa

false

Exposure time: 5s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SR1627

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription factor that recognizes and binds to the AP-1 consensus motif 5'-TGA[GC]TCA-3' (PubMed : 10995748, PubMed : 22083952). Heterodimerizes with proteins of the FOS family to form an AP-1 transcription complex, thereby enhancing its DNA binding activity to the AP-1 consensus sequence 5'-TGA[GC]TCA-3' and enhancing its transcriptional activity (By similarity). Together with FOSB, plays a role in activation-induced cell death of T cells by binding to the AP-1 promoter site of FASLG/CD95L, and inducing its transcription in response to activation of the TCR/CD3 signaling pathway (PubMed : 12618758). Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation (PubMed : 17210646). Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells (PubMed : 24623306). Binds to the USP28 promoter in colorectal cancer (CRC) cells (PubMed : 24623306).. (Microbial infection) Upon Epstein-Barr virus (EBV) infection, binds to viral BZLF1 Z promoter and activates viral BZLF1 expression.
See full target information JUN pS73
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com