Skip to main content

Rabbit Multiclonal c-Jun phospho T91 + T93 + T95 antibody. Suitable for WB, ChIP and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human Transcription factor AP-1 phospho T93 + T95.

Be the first to review this product! Submit a review

Images

ChIP - Anti-c-Jun (phospho T91 + T93 + T95) antibody [RP23040155] - ChIP Grade (AB308130), expandable thumbnail
  • Western blot - Anti-c-Jun (phospho T91 + T93 + T95) antibody [RP23040155] - ChIP Grade (AB308130), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: 99.91% PBS

Form
Liquid
Clonality
Multiclonal

Immunogen

  • Synthetic Peptide within Human Transcription factor AP-1 phospho T93 + T95. Database link P05412

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBChIP
Human
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1-2 µg/mL
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

5 μg on sheared chromatin from 2 million U-937 cells

Target data

Function

Transcription factor that recognizes and binds to the AP-1 consensus motif 5'-TGA[GC]TCA-3' (PubMed:10995748, PubMed:22083952). Heterodimerizes with proteins of the FOS family to form an AP-1 transcription complex, thereby enhancing its DNA binding activity to the AP-1 consensus sequence 5'-TGA[GC]TCA-3' and enhancing its transcriptional activity (By similarity). Together with FOSB, plays a role in activation-induced cell death of T cells by binding to the AP-1 promoter site of FASLG/CD95L, and inducing its transcription in response to activation of the TCR/CD3 signaling pathway (PubMed:12618758). Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation (PubMed:17210646). Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells (PubMed:24623306). Binds to the USP28 promoter in colorectal cancer (CRC) cells (PubMed:24623306). (Microbial infection) Upon Epstein-Barr virus (EBV) infection, binds to viral BZLF1 Z promoter and activates viral BZLF1 expression.

Alternative names

Transcription factor Jun, Activator protein 1, Proto-oncogene c-Jun, Transcription factor AP-1 subunit Jun, V-jun avian sarcoma virus 17 oncogene homolog, p39, AP1, JUN

Recommended products

Rabbit Multiclonal c-Jun phospho T91 + T93 + T95 antibody. Suitable for WB, ChIP and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human Transcription factor AP-1 phospho T93 + T95.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Multiclonal
Immunogen
  • Synthetic Peptide within Human Transcription factor AP-1 phospho T93 + T95. Database link P05412
Clone number
RP23040155
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains.

Recombinant multiclonal antibodies offer the sensitivity of polyclonal antibodies by recognising multiple epitopes, along with consistency of a recombinant antibody.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The protein c-Jun is an important component of the AP-1 transcription factor complex and is often referred to by alternative names such as c-jun. It has a molecular weight of about 39 kDa. Scientists have found c-jun expressed in a wide range of tissues indicating its diverse roles in cellular functions. This protein plays a critical mechanical role by binding specific DNA sequences to regulate the transcription of various genes involved in cell proliferation and apoptosis.

Biological function summary

C-Jun impacts cellular activities by being a significant part of the AP-1 transcription factor complex interacting with other proteins such as Fos. This interaction allows c-Jun to modulate gene expression in response to cellular stimuli. In particular c-Jun influences cell cycle progression and differentiation. Therefore its activity is necessary for physiological and pathological processes. As c-jun's function remains tightly regulated any changes can have wide-ranging effects.

Pathways

C-Jun significantly influences the MAPK/ERK and JNK pathways. These pathways play important roles in cellular responses to stress growth factors and cytokines. Within these pathways c-Jun interacts with proteins such as JNK which phosphorylates c-Jun and enhances its activity. These interactions allow c-Jun to regulate target genes involved in important cellular processes without much delay. Understanding c-Jun's function in these pathways helps elucidate how cells control division survival and apoptosis.

Associated diseases and disorders

C-Jun's dysregulation connects to various conditions including cancer and neurodegenerative diseases. Abnormal c-Jun activity can lead to oncogenesis by promoting unrestrained cellular proliferation. Additionally c-Jun involvement in disorders like Alzheimer's disease stems from its role in apoptotic pathways which may cause neuronal death. Accompanying proteins like JNK are also implicated in these conditions making the regulation and expression of c-Jun a focus for therapeutic research.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • ChIP - Anti-c-Jun (phospho T91 + T93 + T95) antibody [RP23040155] - ChIP Grade (ab308130), expandable thumbnail

    ChIP - Anti-c-Jun (phospho T91 + T93 + T95) antibody [RP23040155] - ChIP Grade (ab308130)

    Enrichment of endogenous c-Jun (phospho T91 + T93 + T95) protein at specific gene loci using ab308130: Chromatin Immunoprecipitation (ChIP) was performed using ab308130, 5 μg on sheared chromatin from 2 million U-937 cells and vs U-937 cells treated with 250 ng/mL anisomycin for 30 minutes using the "MAGnify ChIP system" kit. Normal Rabbit IgG (1 μg) was used as a negative IP control. The purified DNA was analyzed by 7500 Fast qPCR system with optimized PCR primer pairs for the promoters of the active JAG1, MMP13 region used as positive control target genes, and the region of the inactive SAT2 satellite repeat, used as negative control target gene. Data is compared between untreated vs treated samples and is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.

  • Western blot - Anti-c-Jun (phospho T91 + T93 + T95) antibody [RP23040155] - ChIP Grade (ab308130), expandable thumbnail

    Western blot - Anti-c-Jun (phospho T91 + T93 + T95) antibody [RP23040155] - ChIP Grade (ab308130)

    Western blot analysis was performed on whole cell extracts (30 µg lysate) of COLO 205 (Lane 1), U-937 (Lane 2), PC-3 (Lane 3) and U-87 MG (Lane 4). The blots were probed with ab308130, 1-2 µg/mL and detected by chemiluminescence using Goat antiRabbit IgG (H+L) Secondary Antibody, HRP conjugate (0.4 µg/mL, 1:2500 dilution). Two Isoforms of 43 and 36 kDa bands corresponding to c-Jun (phospho T91 + T93 + T95) was observed across cell lines tested. Known quantity of protein samples were electrophoresed using a 4-12% Bis-Tris gel, electrophoresis system and pre-stained protein standard. Resolved proteins were then transferred onto a nitrocellulose membrane. The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed (ECL).

    All lanes: Western blot - Anti-c-Jun (phospho T91 + T93 + T95) antibody [RP23040155] - ChIP Grade (ab308130) at 1 µg/mL

    Lane 1: whole cell extracts of COLO 205 cells at 30 µg

    Lane 2: Whole cell extracts of U-937 cells at 30 µg

    Lane 3: Whole cell extracts of PC-3 cells at 30 µg

    Lane 4: whole cell extracts of U-87 MG cells at 30 µg

    Secondary

    All lanes: Goat antiRabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/2500 dilution

    Developed using the ECL technique.

    Predicted band size: 36 kDa

    Observed band size: 36 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com