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AB212519

Anti-c-Kit antibody [KIT/982] - BSA and Azide free

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(1 Publication)

Mouse Monoclonal c-Kit antibody. Carrier free. Suitable for WB, Protein Array and reacts with Recombinant fragment - Human, Recombinant full length protein - Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human KIT aa 100-300.

View Alternative Names

CD117, SCFR, KIT, Mast/stem cell growth factor receptor Kit, Piebald trait protein, Proto-oncogene c-Kit, Tyrosine-protein kinase Kit, p145 c-kit, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, PBT

2 Images
Protein Array - Anti-c-Kit antibody [KIT/982] - BSA and Azide free (AB212519)
  • Protein Array

Unknown

Protein Array - Anti-c-Kit antibody [KIT/982] - BSA and Azide free (AB212519)

This data was produced with ab233902, the same antibody in a different formulation with BSA and Azide.
ab233902 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score : The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Western blot - Anti-c-Kit antibody [KIT/982] - BSA and Azide free (AB212519)
  • WB

Supplier Data

Western blot - Anti-c-Kit antibody [KIT/982] - BSA and Azide free (AB212519)

All lanes:

Western blot - Anti-c-Kit antibody [KIT/982] - BSA and Azide free (ab212519) at 1 µg/mL

All lanes:

recombinant fragment of Human c-Kit

Predicted band size: 109 kDa,26 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

KIT/982

Isotype

IgG1

Light chain type

kappa

Carrier free

Yes

Applications

WB, Protein Array

applications

Immunogen

Recombinant Fragment Protein within Human KIT aa 100-300. The exact immunogen used to generate this antibody is proprietary information.

P10721

Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Purification notes
Purified from bioreactor concentrates.
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The c-Kit protein also known as CD117 is a type of receptor tyrosine kinase with a molecular weight of approximately 145 kDa. It is encoded by the KIT gene and commonly expressed on the surface of hematopoietic stem cells mast cells melanocytes and germ cells. C-Kit plays a role in the signal transduction pathways that control cell proliferation and survival. Researchers often identify c-Kit using markers like biotin c-Kit PerCP Cy5.5 or antibodies such as anti-c-Kit for experimental purposes.
Biological function summary

C-Kit influences the function and survival of certain cell types. When it binds to its ligand stem cell factor (SCF) it activates receptor dimerization and phosphorylation triggering downstream signaling. C-Kit does not act alone; it often functions in a complex with other proteins to affect cellular outcomes. Its expression is vital for the maintenance of bone marrow hematopoietic cells and contributes to melanocyte and germ cell development.

Pathways

C-Kit interacts with the PI3K/AKT and RAS/ERK pathways through its kinase activity. These pathways play important roles in cell division differentiation and survival. The c-Abl and ACK2 proteins are closely related to c-Kit function within these signaling networks contributing to regulating cellular responses to external stimuli.

C-Kit mutations are frequently associated with gastrointestinal stromal tumors (GIST) and acute myeloid leukemia (AML). These conditions often involve alterations in c-Kit activity leading to uncontrolled cell growth. While studying GIST researchers have noticed interactions between c-Kit and other proteins like CBL which is involved in the modulation of c-Kit signaling. Understanding these interactions helps in designing targeted therapies for conditions involving c-Kit dysregulation.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Tyrosine-protein kinase that acts as a cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Promotes phosphorylation of PIK3R1, CBL, CRK (isoform Crk-II), LYN, MAPK1/ERK2 and/or MAPK3/ERK1, PLCG1, SRC and SHC1.
See full target information KIT

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The EMBO journal 39:e103790 PubMed32567735

2020

Microglia promote glioblastoma via mTOR-mediated immunosuppression of the tumour microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Anaelle A Dumas,Nicola Pomella,Gabriel Rosser,Loredana Guglielmi,Claire Vinel,Thomas O Millner,Jeremy Rees,Natasha Aley,Denise Sheer,Jun Wei,Anantha Marisetty,Amy B Heimberger,Robert L Bowman,Sebastian Brandner,Johanna A Joyce,Silvia Marino
View all publications

Product promise

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