AB62154

Anti-c-Kit (phospho Y703) antibody

Name: Anti-c-Kit (phospho Y703) antibody (ab62154) | Abcam
Brand: Abcam Limited
SKU: AB62154
Price: 545 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(8 Publications)

Rabbit Polyclonal c-Kit phospho Y703 antibody. Suitable for WB and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human KIT phospho Y703.

View Alternative Names

CD117, SCFR, KIT, Mast/stem cell growth factor receptor Kit, Piebald trait protein, Proto-oncogene c-Kit, Tyrosine-protein kinase Kit, p145 c-kit, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, PBT

2 Images

Supplier Data

Western blot - Anti-c-Kit (phospho Y703) antibody (AB62154)

All lanes:

Western blot - Anti-c-Kit (phospho Y703) antibody (ab62154) at 1/500 dilution

Lane 1:

HepG2 (human liver hepatocellular carcinoma cell line) treated with EGF (200ng/ml, 30mins) cell lysate

Lane 2:

HepG2 treated with EGF (200ng/ml, 30mins) cell lysate with immunizing phosphopeptide

Predicted band size: 109 kDa

false

AbReview22276****

Western blot - Anti-c-Kit (phospho Y703) antibody (AB62154)

All lanes:

Western blot - Anti-c-Kit (phospho Y703) antibody (ab62154)

Lane 1:

Colon cancer lysate (human)

Lane 2:

U2OS lysate (human)

Lane 3:

Renal cancer cell line (human)

Secondary

All lanes:

Swine anti-rabbit HRP at 1/5000 dilution

Predicted band size: 109 kDa

true

Exposure time: 6min

This image is courtesy of an Abreview submitted by Antibody Solutions

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human KIT phospho Y703. The exact immunogen used to generate this antibody is proprietary information.

P10721

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab62154 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Storage buffer

pH: 7 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The c-Kit protein also known as CD117 is a type of receptor tyrosine kinase with a molecular weight of approximately 145 kDa. It is encoded by the KIT gene and commonly expressed on the surface of hematopoietic stem cells mast cells melanocytes and germ cells. C-Kit plays a role in the signal transduction pathways that control cell proliferation and survival. Researchers often identify c-Kit using markers like biotin c-Kit PerCP Cy5.5 or antibodies such as anti-c-Kit for experimental purposes.

Biological function summary

C-Kit influences the function and survival of certain cell types. When it binds to its ligand stem cell factor (SCF) it activates receptor dimerization and phosphorylation triggering downstream signaling. C-Kit does not act alone; it often functions in a complex with other proteins to affect cellular outcomes. Its expression is vital for the maintenance of bone marrow hematopoietic cells and contributes to melanocyte and germ cell development.

Pathways

C-Kit interacts with the PI3K/AKT and RAS/ERK pathways through its kinase activity. These pathways play important roles in cell division differentiation and survival. The c-Abl and ACK2 proteins are closely related to c-Kit function within these signaling networks contributing to regulating cellular responses to external stimuli.

C-Kit mutations are frequently associated with gastrointestinal stromal tumors (GIST) and acute myeloid leukemia (AML). These conditions often involve alterations in c-Kit activity leading to uncontrolled cell growth. While studying GIST researchers have noticed interactions between c-Kit and other proteins like CBL which is involved in the modulation of c-Kit signaling. Understanding these interactions helps in designing targeted therapies for conditions involving c-Kit dysregulation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Tyrosine-protein kinase that acts as a cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Promotes phosphorylation of PIK3R1, CBL, CRK (isoform Crk-II), LYN, MAPK1/ERK2 and/or MAPK3/ERK1, PLCG1, SRC and SHC1.

See full target information KIT phospho Y703

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Oncology reports 47: PubMed34738628

2021

Glucose transporter‑1 inhibition overcomes imatinib resistance in gastrointestinal stromal tumor cells.

Applications

Unspecified application

Species

Unspecified reactive species

Takafumi Shima,Kohei Taniguchi,Yoshihisa Tokumaru,Yosuke Inomata,Jun Arima,Sang-Woong Lee,Kazuaki Takabe,Kazuhiro Yoshida,Kazuhisa Uchiyama

Development (Cambridge, England) 146: PubMed31548215

2019

SCF/SCFR signaling plays an important role in the early morphogenesis and neurogenesis of human embryonic neural retina.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Gong,Xiangyu He,Qiyou Li,Juncai He,Baishijiao Bian,Yijian Li,Linlin Ge,Yuxiao Zeng,Haiwei Xu,Zheng Qin Yin

Oncology letters 17:3071-3076 PubMed30867736

2019

Predictive and prognostic value of phosphorylated c-KIT and PDGFRA in advanced non-small cell lung cancer harboring ALK fusion.

Applications

Unspecified application

Species

Unspecified reactive species

Haihong Yang,Fengnan Wang,Qiuhua Deng,Dakai Xiao,Ping He,Xiaodong Lin,Dongyun He

Journal of dairy science 102:26-36 PubMed30527985

2018

Lactobacillus plantarum YS2 (yak yogurt Lactobacillus) exhibited an activity to attenuate activated carbon-induced constipation in male Kunming mice.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Zhao,Yu Qian,Guijie Li,Ruokun Yi,Kun-Young Park,Jia-Le Song

Experimental and therapeutic medicine 13:1093-1100 PubMed28450948

2017

Preventative effects of fermented shoot on activated carbon-induced constipation.

Applications

Species

Unspecified reactive species

Guijie Li,Xiaochuan Zou,Gang Kuang,Yanrong Ren,Chaofang Deng,Qiang Lin,Xin Zhao,Song Xu,Jia-Le Song

Reproduction (Cambridge, England) 153:211-220 PubMed27864336

2016

BMP4 promotes mouse iPS cell differentiation to male germ cells via Smad1/5, Gata4, Id1 and Id2.

Applications

Unspecified application

Species

Unspecified reactive species

Shi Yang,Qingqing Yuan,Minghui Niu,Jingmei Hou,Zijue Zhu,Min Sun,Zheng Li,Zuping He

Heart and vessels 31:795-806 PubMed26129868

2015

Histopathology of aortic complications in bicuspid aortic valve versus Marfan syndrome: relevance for therapy?

Applications

Unspecified application

Species

Unspecified reactive species

Nimrat Grewal,Romy Franken,Barbara J M Mulder,Marie-José Goumans,Johannes H N Lindeman,Monique R M Jongbloed,Marco C DeRuiter,Robert J M Klautz,Ad J J C Bogers,Robert E Poelmann,Adriana C Gittenberger-de Groot

European journal of cardio-thoracic surgery : offi 46:831-9 PubMed25161185

2014

Bicuspid aortic valve: phosphorylation of c-Kit and downstream targets are prognostic for future aortopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Nimrat Grewal,Adriana C Gittenberger-de Groot,Marco C DeRuiter,Robert J M Klautz,Robert E Poelmann,Sjoerd Duim,Johannes H N Lindeman,Wilke M C Koenraadt,Monique R M Jongbloed,Salah A Mohamed,Hans-Hinrich Sievers,Ad J J C Bogers,Marie-José Goumans

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-c-Kit (phospho Y703) antibody

Western blot - Anti-c-Kit (phospho Y703) antibody (AB62154)

Western blot - Anti-c-Kit (phospho Y703) antibody (AB62154)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (8)

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