Rabbit Polyclonal c-Maf antibody. Suitable for IHC-P and reacts with Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human MAF aa 250-350 conjugated to Keyhole Limpet Haemocyanin.
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- Biochemical and biophysical research communication 514:1140-11462019Long non-coding RNA ANGPTL1-3 promotes multiple myeloma bortezomib resistance by sponging miR-30a-3p to activate c-Maf expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFeige Nian et. al.PubMed 31103265
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human MAF aa 250-350 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link O75444
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Reactivity data
Select an application| IHC-P | |
|---|---|
| Mouse | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 - 1/500 | Notes When using a fluorescent probe the recommended dilution is 1/50 – 1/200. |
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Target data
Function
Acts as a transcriptional activator or repressor. Involved in embryonic lens fiber cell development. Recruits the transcriptional coactivators CREBBP and/or EP300 to crystallin promoters leading to up-regulation of crystallin gene during lens fiber cell differentiation. Activates the expression of IL4 in T helper 2 (Th2) cells. Increases T-cell susceptibility to apoptosis by interacting with MYB and decreasing BCL2 expression. Together with PAX6, transactivates strongly the glucagon gene promoter through the G1 element. Activates transcription of the CD13 proximal promoter in endothelial cells. Represses transcription of the CD13 promoter in early stages of myelopoiesis by affecting the ETS1 and MYB cooperative interaction. Involved in the initial chondrocyte terminal differentiation and the disappearance of hypertrophic chondrocytes during endochondral bone development. Binds to the sequence 5'-[GT]G[GC]N[GT]NCTCAGNN-3' in the L7 promoter. Binds to the T-MARE (Maf response element) sites of lens-specific alpha- and beta-crystallin gene promoters. Binds element G1 on the glucagon promoter. Binds an AT-rich region adjacent to the TGC motif (atypical Maf response element) in the CD13 proximal promoter in endothelial cells (By similarity). When overexpressed, represses anti-oxidant response element (ARE)-mediated transcription. Involved either as an oncogene or as a tumor suppressor, depending on the cell context. Binds to the ARE sites of detoxifying enzyme gene promoters.
Alternative names
Transcription factor Maf, Proto-oncogene c-Maf, V-maf musculoaponeurotic fibrosarcoma oncogene homolog, MAF
Recommended products
Rabbit Polyclonal c-Maf antibody. Suitable for IHC-P and reacts with Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human MAF aa 250-350 conjugated to Keyhole Limpet Haemocyanin.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human MAF aa 250-350 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link O75444
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The c-Maf protein also known as Maf or MAF is a member of the large MAF family of basic leucine zipper (bZIP) transcription factors. This protein plays a significant role in the regulation of gene expression. It has a molecular weight of approximately 38 kDa. c-Maf is commonly expressed in various tissues such as the lens of the eye the kidneys and specific immune cells including T cells and macrophages. It is widely studied due to its involvement in transcriptional activation of certain target genes.
- Biological function summary
C-Maf protein influences cellular differentiation and function particularly in immune cells. It forms part of a transcriptional complex that regulates genes critical for the production of interleukins and other cytokines. In the immune system c-Maf is essential for the differentiation of T-helper 2 cells and influences the production of cytokines such as IL-4. Additionally it is involved in the maturation of lens fibers in the eye and modulation of renal functions.
- Pathways
C-Maf plays an important role in the immune and visual system. It is part of the signaling pathways that regulate Th2 cytokine genes including IL-4 within the adaptive immune response. This process involves interaction with other transcription factors such as STAT6 and GATA3. Moreover the lens development pathway also includes c-Maf where it works with proteins like CRYAA to maintain lens transparency and function.
- Associated diseases and disorders
C-Maf has been linked to multiple myeloma and juvenile idiopathic arthritis. c-Maf is often upregulated in multiple myeloma a cancer of plasma cells where it influences proliferation and survival of the malignant cells through its target genes like cyclin D2. Furthermore in juvenile idiopathic arthritis c-Maf contributes to the dysregulation of inflammatory cytokines indirectly involving proteins like IL-10 and IL-21. Understanding c-Maf’s function aids in exploring therapeutic targets for these diseases.
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Tested
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Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Maf antibody (ab203885)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse placenta tissue labeling c-Maf with ab203885 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.
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