AB199424

Anti-c-Maf antibody [EPR16484]

RabMAb
Recombinant
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(7 Publications)

Rabbit Recombinant Monoclonal c-Maf antibody. Suitable for ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 7 publications.

View Alternative Names

Transcription factor Maf, Proto-oncogene c-Maf, V-maf musculoaponeurotic fibrosarcoma oncogene homolog, MAF

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Maf antibody [EPR16484] (AB199424)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Maf antibody [EPR16484] (AB199424)

Immunohistochemical analysis of paraffin-embedded Human pancreas tissue using ab199424 at 1/500 followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500. No staining on Human pancreas tissue is observed (Subcellular location : Nucleus [UniProt]). Counter stained with Hematoxylin. Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Negative control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Maf antibody [EPR16484] (AB199424)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling c-Maf with ab199424 at 1/500 followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500. Nucleus staining on epithelial on Human tonsil tissue is observed (Subcellular location : Nucleus [UniProt]). Counter stained with Hematoxylin. Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Negative control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-c-Maf antibody [EPR16484] (AB199424)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-c-Maf antibody [EPR16484] (AB199424)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 786-O (human kidney epithelial cell) cells labelling c-Maf with ab199424 at 1/50 dilution (10.34 μg/ml) followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed secondary antibody used at 1/1000 dilution (2µg/ml) (Green). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5µg/ml)(Red). The Nuclear counterstain was DAPI (Blue). Confocal image showing nuclear staining in 786-O cell line Negative control : HeLa (PMID : 28933784）

Flow Cytometry (Intracellular) - Anti-c-Maf antibody [EPR16484] (AB199424)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-c-Maf antibody [EPR16484] (AB199424)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervical adenocarcinoma epithelial cell, Left) / 786-O (human kidney epithelial cell, Right) cells labelling c-Maf with ab199424 at 1/50 dilution (1 ug)/Red compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody. Negative control : HeLa (PMID : 28933784）

Carrier free

Anti-c-Maf antibody [EPR16484] - BSA and Azide free
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-c-Maf antibody [EPR16484]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16484

Isotype

IgG

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra), ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The c-Maf protein also known as Maf or MAF is a member of the large MAF family of basic leucine zipper (bZIP) transcription factors. This protein plays a significant role in the regulation of gene expression. It has a molecular weight of approximately 38 kDa. c-Maf is commonly expressed in various tissues such as the lens of the eye the kidneys and specific immune cells including T cells and macrophages. It is widely studied due to its involvement in transcriptional activation of certain target genes.

Biological function summary

C-Maf protein influences cellular differentiation and function particularly in immune cells. It forms part of a transcriptional complex that regulates genes critical for the production of interleukins and other cytokines. In the immune system c-Maf is essential for the differentiation of T-helper 2 cells and influences the production of cytokines such as IL-4. Additionally it is involved in the maturation of lens fibers in the eye and modulation of renal functions.

Pathways

C-Maf plays an important role in the immune and visual system. It is part of the signaling pathways that regulate Th2 cytokine genes including IL-4 within the adaptive immune response. This process involves interaction with other transcription factors such as STAT6 and GATA3. Moreover the lens development pathway also includes c-Maf where it works with proteins like CRYAA to maintain lens transparency and function.

C-Maf has been linked to multiple myeloma and juvenile idiopathic arthritis. c-Maf is often upregulated in multiple myeloma a cancer of plasma cells where it influences proliferation and survival of the malignant cells through its target genes like cyclin D2. Furthermore in juvenile idiopathic arthritis c-Maf contributes to the dysregulation of inflammatory cytokines indirectly involving proteins like IL-10 and IL-21. Understanding c-Maf’s function aids in exploring therapeutic targets for these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Acts as a transcriptional activator or repressor. Involved in embryonic lens fiber cell development. Recruits the transcriptional coactivators CREBBP and/or EP300 to crystallin promoters leading to up-regulation of crystallin gene during lens fiber cell differentiation. Activates the expression of IL4 in T helper 2 (Th2) cells. Increases T-cell susceptibility to apoptosis by interacting with MYB and decreasing BCL2 expression. Together with PAX6, transactivates strongly the glucagon gene promoter through the G1 element. Activates transcription of the CD13 proximal promoter in endothelial cells. Represses transcription of the CD13 promoter in early stages of myelopoiesis by affecting the ETS1 and MYB cooperative interaction. Involved in the initial chondrocyte terminal differentiation and the disappearance of hypertrophic chondrocytes during endochondral bone development. Binds to the sequence 5'-[GT]G[GC]N[GT]NCTCAGNN-3' in the L7 promoter. Binds to the T-MARE (Maf response element) sites of lens-specific alpha- and beta-crystallin gene promoters. Binds element G1 on the glucagon promoter. Binds an AT-rich region adjacent to the TGC motif (atypical Maf response element) in the CD13 proximal promoter in endothelial cells (By similarity). When overexpressed, represses anti-oxidant response element (ARE)-mediated transcription. Involved either as an oncogene or as a tumor suppressor, depending on the cell context. Binds to the ARE sites of detoxifying enzyme gene promoters.

See full target information MAF

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Toxicologic pathology 52:266-283 PubMed39193778

2024

Biocompatible Solutions: Evaluating the Safety of Repeated Intra-Articular Injections of pMPCylated Liposomes for Knee Osteoarthritis Therapy in Rat Models.

Applications

Unspecified application

Species

Unspecified reactive species

Yuval Ramot,Noam Kronfeld,Michal Steiner,Eric D Lee,Ronit Goldberg,Sabrina Jahn,Abraham Nyska

International journal of molecular sciences 23: PubMed36293536

2022

Macrophage Phenotypes and Gene Expression Patterns Are Unique in Naturally Occurring Metabolically Healthy Obesity.

Applications

Unspecified application

Species

Unspecified reactive species

Alistaire D Ruggiero,Ravichandra Vemuri,Masha Block,Darla DeStephanis,Matthew Davis,Jeff Chou,Abigail Williams,Ashlynn Brock,Swapan Kumar Das,Kylie Kavanagh

Evidence-based complementary and alternative medicine : eCAM 2022:7861338 PubMed35341136

2022

Liquiritin Attenuates Angiotensin II-Induced Cardiomyocyte Hypertrophy via ATE1/TAK1-JNK1/2 Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jiajia Mo,Peng Zhou,Zhaoxing Chu,Yan Zhao,Xiang Wang

Cancers 13: PubMed34830831

2021

Immunosuppressive Microenvironment and Efficacy of PD-1 Inhibitors in Relapsed/Refractory Classic Hodgkin Lymphoma: Checkpoint Molecules Landscape and Macrophage Populations.

Applications

Unspecified application

Species

Unspecified reactive species

Artem Gusak,Liudmila Fedorova,Kirill Lepik,Nikita Volkov,Marina Popova,Ivan Moiseev,Natalia Mikhailova,Vadim Baykov,Alexander Kulagin

International journal of radiation oncology, biology, physics 111:249-259 PubMed33848608

2021

Clinicopathologic and Transcriptomic Analysis of Radiation-Induced Lung Injury in Nonhuman Primates.

Applications

Unspecified application

Species

Unspecified reactive species

Priyanka Thakur,Ryne DeBo,Gregory O Dugan,J Daniel Bourland,Kris T Michalson,John D Olson,Thomas C Register,Nancy D Kock,J Mark Cline

Journal of clinical and experimental hematopathology : JCEH 59:112-118 PubMed31564713

2019

Maf expression in human macrophages and lymph node sinus macrophages in patients with esophageal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Hiroto Takeya,Koji Ohnishi,Takuya Shiota,Yoichi Saito,Yukio Fujiwara,Taisuke Yagi,Yuki Kiyozumi,Yoshifumi Baba,Naoya Yoshida,Kenichi Asano,Masato Tanaka,Hideo Baba,Yoshihiro Komohara

Cancer science 110:2600-2606 PubMed31218784

2019

Immunohistochemistry for identification of CCND1, NSD2, and MAF gene rearrangements in plasma cell myeloma.

Applications

Unspecified application

Species

Unspecified reactive species

Takayuki Murase,Masaki Ri,Tomoko Narita,Keiichiro Fujii,Ayako Masaki,Shinsuke Iida,Hiroshi Inagaki

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-c-Maf antibody [EPR16484]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Maf antibody [EPR16484] (AB199424)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Maf antibody [EPR16484] (AB199424)

Immunocytochemistry/ Immunofluorescence - Anti-c-Maf antibody [EPR16484] (AB199424)

Flow Cytometry (Intracellular) - Anti-c-Maf antibody [EPR16484] (AB199424)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

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