Anti-c-Met (phospho Y1349) antibody [RP23040111]

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-c-Met (phospho Y1349) antibody [RP23040111] (AB313438)

Immunofluorescent analysis of MKN45 cells fixed using 4% formaldehyde (reconstituted in 1X PBS) for 10 min at room temperature and permeabilized using 0.1 % Triton X-100 in PBS for 15 min at room temperature which were serum starved for 16 hours and treated with HGF (100 ng for 10 min) labeling cMet (pY1349) with ab313438 at 1-2 µg/mL followed by Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor 488 conjugate at 1/2000 dilution. Panel a) shows representative cells that were stained for detection and localization of cMet (pY1349) protein (green), Panel b) is stained for nuclei (blue) using DAPI. Panel c) represents cytoskeletal F-actin staining using Alexa Fluor 555 Rhodamine Phalloidin at 1/300 dilution. Panel d) is a composite image of Panels a, b and c clearly demonstrating cytoplasmic localization of cMet (pY1349). Panel e) represents merged image of untreated cells with no signal. Panel f) represents control cells with no primary Antibody to assess background.

• WB

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Western blot - Anti-c-Met (phospho Y1349) antibody [RP23040111] (AB313438)

All lanes:

Western blot - Anti-c-Met (phospho Y1349) antibody [RP23040111] (ab313438) at 1 µg/mL

Lane 1:

MKN 45 cell lysate at 50 µg

Lane 2:

MKN 45 cell lysate treated with HGF (serum starved/ON, 100 ng/10 min) at 50 µg

Secondary

All lanes:

Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/2500 dilution

Observed band size: 156 kDa

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