Anti-c-Myb antibody

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-c-Myb antibody (AB117635)

Immunofluorescence analysis of HeLa cells, using 1/100 ab117635.
The picture on the right is treated with the synthesized peptide.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myb antibody (AB117635)

Human Breast, Formalin-Fixed, Paraffin-Embedded tissue, stained with ab117635 at 5μg/ml.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myb antibody (AB117635)

Human Tonsil, Formalin-Fixed, Paraffin-Embedded tissue, stained with ab117635 at 5μg/ml.

• WB

Unknown

Western blot - Anti-c-Myb antibody (AB117635)

All lanes:

Western blot - Anti-c-Myb antibody (ab117635) at 1/500 dilution

Lane 1:

HUVEC cell lysate

Lane 2:

HUVEC cell lysate with synthetic peptide

Predicted band size: 72 kDa

false

• WB

CiteAb

Western blot - Anti-c-Myb antibody (AB117635)

c-Myb western blot using anti-c-Myb antibody ab117635. Publication image and figure legend from Felipe-Abrio, B., Verdugo-Sivianes, E. M., et al., 2019, Cancers (Basel), PubMed 30781655.

ab117635 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab117635 please see the product overview.

Characterization of MYBBP1A expression and MYBBP1A binding to c-MYB in renal carcinoma cell lines. (A) Measurement of MYBBP1A, c-MYB, p53 and pVHL levels in renal carcinoma cell lines A498, 786-O, ACHN and CaKi-1 by western blot. Cells were cultured in 4500 mg/L glucose media. (B) Measurement of MYBBP1A, c-MYB, p53, acetyl-p53 and pVHL levels in A498, 786-O, ACHN and CaKi-1 by western blot. Cells were cultured in 1000 mg/L or 100 mg/L glucose media. (C) MYBBP1A and c-MYB co-localize in the nucleolus of renal carcinoma cell lines. Cells were stained using DAPI (nuclear control), MYBBP1A and c-MYB antibodies. Scale Bar : 8 µM. (D) Co-immunoprecipitation of c-MYB and MYBBP1A in renal carcinoma cells. Protein extracts from 786-O and A498 were subject to immunoprecipitation with c-MYB or normal IgG antibodies. The resultant immunoprecipitates were then analyzed by WB with c-MYB and MYBBP1A antibodies.

false