AB226470

Anti-c-Myb antibody

Name: Anti-c-Myb antibody (ab226470) | Abcam
Brand: Abcam Limited
SKU: AB226470
Price: 525 USD
Availability: InStock

Be the first to review this product! Submit a review

(6 Publications)

Rabbit Polyclonal c-Myb antibody. Suitable for IHC-P, IP and reacts with Mouse, Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human MYB aa 1-50.

View Alternative Names

Transcriptional activator Myb, Proto-oncogene c-Myb, MYB

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myb antibody (AB226470)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myb antibody (AB226470)

Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for c-Myb using ab226470 at 1/5000 dilution in immunohistochemical analysis.

Detection : DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myb antibody (AB226470)

Formalin-fixed, paraffin-embedded human salivary gland tissue stained for c-Myb using ab226470 at 1/1000 dilution in immunohistochemical analysis.

Detection : DAB staining.

Supplier Data

Immunoprecipitation - Anti-c-Myb antibody (AB226470)

c-Myb was immunoprecipitated from MOLT-4 (human lymphoblastic leukemia cell line) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226470 at 6 μg/mg lysate. Western blot was performed from the immunoprecipitate using a different rabbit anti-c-Myb antibody at 1 μg/ml.

Lane 1 : ab226470 IP in MOLT-4 whole cell lysate.

Lane 2 : Control IgG IP in MOLT-4 whole cell lysate.

Detection : Chemiluminescence with exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-c-Myb antibody (ab226470)

Predicted band size: 72 kDa

false

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myb antibody (AB226470)

Formalin-fixed, paraffin-embedded mouse renal cell carcinoma tissue stained for c-Myb using ab226470 at 1/1000 dilution in immunohistochemical analysis.

Detection : DAB staining.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, IP

applications

Immunogen

Synthetic Peptide within Human MYB aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

P10242

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/5000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/5000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Horse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab226470 was affinity purified using an epitope specific to c-Myb immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Do Not Freeze

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The c-Myb protein also known as Myb MYB or c-myb acts mechanically as a transcription factor that regulates the expression of target genes. It plays a role in cellular processes like proliferation differentiation and apoptosis by binding to specific DNA sequences. c-Myb has a molecular mass of roughly 75 kDa. It predominantly expresses in hematopoietic cells where it significantly influences gene transcription activity. Also it appears in some epithelial tissues indicating its versatile role in different cell types.

Biological function summary

The protein serves as a master regulator in the development of various cell types. It is not part of a traditional complex but interacts with other proteins to exert its function such as binding with co-activators or repressors to modulate gene expression. c-Myb controls the transcription of genes that are critical for cell cycle regulation and differentiation especially in the context of blood cell lineages. The protein is particularly influential in the production of red blood cells and white blood cells.

Pathways

C-Myb fits integrally into known hematopoietic signaling cascades and the Wnt signaling pathway. It works alongside other proteins like cyclin-dependent kinase (CDK) family members and transcription factor GATA-1 to modulate lineage-specific gene expression. Through its regulatory actions c-Myb influences the activation of downstream effectors that propagate signals essential for maintaining cellular functions within these pathways. The modulation of these pathways underlines its role in cell fate decisions.

Studies link c-Myb to several forms of leukemia and colon cancer. In leukemia aberrant expression of c-Myb disrupts normal hematopoiesis contributing to the uncontrolled proliferation of immature blood cells. The abnormal interactions between c-Myb and proteins like Bcl-2 can lead to enhanced cellular survival further promoting malignant conditions. In colon cancer c-Myb overexpression influences tumor growth and progression often acting alongside other oncogenic factors to enhance disease severity.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Transcriptional activator; DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'. Plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells.

See full target information MYB

style

left-column

Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Biology direct 19:133 PubMed39709463

2024

MLL1 promotes placental trophoblast ferroptosis and aggravates preeclampsia symptoms through epigenetic regulation of RBM15/TRIM72/ADAM9 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Lingling Li,Haining He,Zhenrong Zheng,Xiaolan Zhao

iScience 26:108487 PubMed38089573

2023

MYB exhibits racially disparate expression, clinicopathologic association, and predictive potential for biochemical recurrence in prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Mohammad Aslam Khan,Srijan Acharya,Shashi Anand,Fnu Sameeta,Paramahansa Pramanik,Christopher Keel,Seema Singh,James Elliot Carter,Santanu Dasgupta,Ajay Pratap Singh

International journal of molecular sciences 24: PubMed37762093

2023

rs71327024 Associated with COVID-19 Hospitalization Reduces Promoter Activity in Human CD4 T Cells via Disruption of c-Myb Binding.

Applications

Unspecified application

Species

Unspecified reactive species

Aksinya N Uvarova,Ekaterina M Stasevich,Alina S Ustiugova,Nikita A Mitkin,Elina A Zheremyan,Savely A Sheetikov,Ksenia V Zornikova,Apollinariya V Bogolyubova,Mikhail A Rubtsov,Ivan V Kulakovskiy,Dmitry V Kuprash,Kirill V Korneev,Anton M Schwartz

Frontiers in cell and developmental biology 11:1168866 PubMed37701782

2023

Expression dynamics of metalloproteinases during mandibular bone formation: association with Myb transcription factor.

Applications

Unspecified application

Species

Unspecified reactive species

S Varadinkova,V Oralova,M Clarke,J Frampton,L Knopfova,H Lesot,P Bartos,E Matalova

Cancer immunology research 11:1432-1444 PubMed37478172

2023

Tumor Cell-Intrinsic c-Myb Upregulation Stimulates Antitumor Immunity in a Murine Colorectal Cancer Model.

Applications

Unspecified application

Species

Unspecified reactive species

Merel van Gogh,Jesus F Glaus Garzon,Dilara Sahin,Lucia Knopfova,Petr Benes,Onur Boyman,Igor Jurisica,Lubor Borsig

Experimental & molecular medicine 51:1-16 PubMed31409771

2019

Sex-determining region Y (SRY) attributes to gender differences in RANKL expression and incidence of osteoporosis.

Applications

Unspecified application

Species

Unspecified reactive species

Klemen Kodrič,Janja Zupan,Tilen Kranjc,Radko Komadina,Vid Mlakar,Janja Marc,Nika Lovšin

View all publications

style

left-column

style

left-column

style

right-column

Alternative Products

Primary Antibodies

AB177510

Anti-c-Myb antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myb antibody (AB177510)

Primary Antibodies

AB169111

Anti-c-Myb antibody [ANA236B]

KO Validated

Western blot - Anti-c-Myb antibody [ANA236B] (AB169111)

Primary Antibodies

AB109127

Anti-c-Myb antibody [EPR718(2)]

RabMAb|Recombinant|KO Validated

Western blot - Anti-c-Myb antibody [EPR718(2)] (AB109127)

Primary Antibodies

AB45150

Anti-c-Myb (phospho S11) antibody [EP769Y]

RabMAb|Recombinant

Western blot - Anti-c-Myb (phospho S11) antibody [EP769Y] (AB45150)

Primary Antibodies

AB239947

Anti-c-Myb antibody [EPR718(2)] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Western blot - Anti-c-Myb antibody [EPR718(2)] - BSA and Azide free (AB239947)

Primary Antibodies

AB117635

Anti-c-Myb antibody

Immunocytochemistry/ Immunofluorescence - Anti-c-Myb antibody (AB117635)

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Primary Antibodies

AB7817

Anti-alpha smooth muscle Actin antibody [1A4]

KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] (AB7817)

Primary Antibodies

AB110641

Anti-BRG1 antibody [EPNCIR111A]

BOND RX™ Validated|Recombinant|KO Validated|Advanced Validation|RabMAb

ChIC/CUT&RUN sequencing - Anti-BRG1 antibody [EPNCIR111A] (AB110641)

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com