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AB239947

Anti-c-Myb antibody [EPR718(2)] - BSA and Azide free

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Rabbit Recombinant Monoclonal c-Myb antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

Transcriptional activator Myb, Proto-oncogene c-Myb, MYB

2 Images
Western blot - Anti-c-Myb antibody [EPR718(2)] - BSA and Azide free (AB239947)
  • WB

Unknown

Western blot - Anti-c-Myb antibody [EPR718(2)] - BSA and Azide free (AB239947)

This data was developed using ab109127, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-c-Myb antibody [EPR718(2)] (<a href='/en-us/products/primary-antibodies/c-myb-antibody-epr7182-ab109127'>ab109127</a>) at 1/1000 dilution

Lane 1:

Jurkat cell lysates at 10 µg

Lane 2:

MOLT4 cell lysates at 10 µg

Predicted band size: 72 kDa

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Western blot - Anti-c-Myb antibody [EPR718(2)] - BSA and Azide free (AB239947)
  • WB

Lab

Western blot - Anti-c-Myb antibody [EPR718(2)] - BSA and Azide free (AB239947)

This data was developed using ab109127, the same antibody clone in a different buffer formulation.

Lane 1 : Wild-type HAP1 whole cell lysate (20 μg)

Lane 2 : c-Myb knockout HAP1 whole cell lysate (20 μg)

Lanes 1 - 2 : Merged signal (red and green). Green - ab109127 observed at 75 kDa. Red - loading control, ab9484, observed at 37 kDa.

ab109127 was shown to specifically react with c-Myb in wild-type HAP1 cells as signal was lost in c-Myb knockout cells. Wild-type and c-Myb knockout samples were subjected to SDS-PAGE. ab109127 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-c-Myb antibody [EPR718(2)] (<a href='/en-us/products/primary-antibodies/c-myb-antibody-epr7182-ab109127'>ab109127</a>)

Predicted band size: 72 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR718(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab239947 is the carrier-free version of ab109127.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The c-Myb protein also known as Myb MYB or c-myb acts mechanically as a transcription factor that regulates the expression of target genes. It plays a role in cellular processes like proliferation differentiation and apoptosis by binding to specific DNA sequences. c-Myb has a molecular mass of roughly 75 kDa. It predominantly expresses in hematopoietic cells where it significantly influences gene transcription activity. Also it appears in some epithelial tissues indicating its versatile role in different cell types.
Biological function summary

The protein serves as a master regulator in the development of various cell types. It is not part of a traditional complex but interacts with other proteins to exert its function such as binding with co-activators or repressors to modulate gene expression. c-Myb controls the transcription of genes that are critical for cell cycle regulation and differentiation especially in the context of blood cell lineages. The protein is particularly influential in the production of red blood cells and white blood cells.

Pathways

C-Myb fits integrally into known hematopoietic signaling cascades and the Wnt signaling pathway. It works alongside other proteins like cyclin-dependent kinase (CDK) family members and transcription factor GATA-1 to modulate lineage-specific gene expression. Through its regulatory actions c-Myb influences the activation of downstream effectors that propagate signals essential for maintaining cellular functions within these pathways. The modulation of these pathways underlines its role in cell fate decisions.

Studies link c-Myb to several forms of leukemia and colon cancer. In leukemia aberrant expression of c-Myb disrupts normal hematopoiesis contributing to the uncontrolled proliferation of immature blood cells. The abnormal interactions between c-Myb and proteins like Bcl-2 can lead to enhanced cellular survival further promoting malignant conditions. In colon cancer c-Myb overexpression influences tumor growth and progression often acting alongside other oncogenic factors to enhance disease severity.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcriptional activator; DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'. Plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells.
See full target information MYB
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Product promise

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