JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB45150

Anti-c-Myb (phospho S11) antibody [EP769Y]

4

(1 Review)

|

(68 Publications)

Anti-c-Myb (phospho S11) antibody [EP769Y] (ab45150) is a rabbit monoclonal antibody detecting c-Myb in Western Blot, IP, IHC-P, Dot Blot. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency
- Over 60 publications
- Trusted since 2006

View Alternative Names

Transcriptional activator Myb, Proto-oncogene c-Myb, MYB

9 Images
Western blot - Anti-c-Myb (phospho S11) antibody [EP769Y] (AB45150)
  • WB

Lab

Western blot - Anti-c-Myb (phospho S11) antibody [EP769Y] (AB45150)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-c-Myb (phospho S11) antibody [EP769Y] (ab45150) at 1/5000 dilution

Lane 1:

Untreated Ramos (Human Burkitt's lymphoma cell line) cell lysate at 10 µg

Lane 2:

Ramos cell lysate, membrane treated with alkaline phosphatase at 10 µg

Secondary

All lanes:

HRP goat anti-rabbit IgG (H+L) at 1/2000 dilution

Predicted band size: 72 kDa

Observed band size: 75 kDa,77 kDa

false

Exposure time: 3min

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myb (phospho S11) antibody [EP769Y] (AB45150)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myb (phospho S11) antibody [EP769Y] (AB45150)

Immunohistochemical analysis of paraffin-embedded human vervical carcinoma tissue staining c-Myb with unpurified ab45150.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunoprecipitation - Anti-c-Myb (phospho S11) antibody [EP769Y] (AB45150)
  • IP

Lab

Immunoprecipitation - Anti-c-Myb (phospho S11) antibody [EP769Y] (AB45150)

c-Myb was immunoprecipitated from 0.35 mg MOLT-4 (Human lymphoblastic leukemia T lymphoblast) whole cell lysate 10 μg with ab45150 at 1/120 dilution (2μg). VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 : MOLT-4 (Human lymphoblastic leukemia T lymphoblast) whole cell lysate 10 μg

Lane 2 : ab45150 IP in MOLT-4 whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab45150 in MOLT-4 whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Fresh lysate should be used to minimize protein degradation. Multi-bands should be c-myb isoforms based on immunogen design and Uniprot database.

All lanes:

Immunoprecipitation - Anti-c-Myb (phospho S11) antibody [EP769Y] (ab45150)

Predicted band size: 72 kDa

Observed band size: 75-77 kDa

false

Western blot - Anti-c-Myb (phospho S11) antibody [EP769Y] (AB45150)
  • WB

Lab

Western blot - Anti-c-Myb (phospho S11) antibody [EP769Y] (AB45150)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-c-Myb (phospho S11) antibody [EP769Y] (ab45150) at 1/2000 dilution

Lane 1:

HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate at 10 µg with c-Myc (phospho S11) peptide

Lane 2:

HeLa cell lysate at 10 µg with c-Myc (phospho S12) peptide

Lane 3:

HeLa cell lysate at 10 µg with c-Myc (phospho S11/12) peptide

Lane 4:

HeLa cell lysate at 10 µg with c-Myc unmodified peptide

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 72 kDa

Observed band size: 75 kDa,77 kDa

false

Western blot - Anti-c-Myb (phospho S11) antibody [EP769Y] (AB45150)
  • WB

Lab

Western blot - Anti-c-Myb (phospho S11) antibody [EP769Y] (AB45150)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-c-Myb (phospho S11) antibody [EP769Y] (ab45150) at 1/2000 dilution

Lane 1:

HeLa cell lysate, the membrane treated with lambda phosphatase at 10 µg

Lane 2:

Untreated HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/5000 dilution

Predicted band size: 72 kDa

Observed band size: 75 kDa,77 kDa

false

Exposure time: 30s

Western blot - Anti-c-Myb (phospho S11) antibody [EP769Y] (AB45150)
  • WB

Lab

Western blot - Anti-c-Myb (phospho S11) antibody [EP769Y] (AB45150)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-c-Myb (phospho S11) antibody [EP769Y] (ab45150) at 1/2000 dilution

Lane 1:

Molt-4 (Human lymphoblastic leukemia cell line) cell lysate at 10 µg with c-Myb (phospho S11) peptide

Lane 2:

Molt-4 cell lysate at 10 µg with c-Myb (phospho S12) peptide

Lane 3:

Molt-4 cell lysate at 10 µg with c-Myb (phospho S11/12) peptide

Lane 4:

Molt-4 cell lysate at 10 µg with c-Myb unmodified peptide

Secondary

All lanes:

HRP goat anti-rabbit IgG (H+L) at 1/2000 dilution

Predicted band size: 72 kDa

Observed band size: 77 kDa

false

Exposure time: 30s

Western blot - Anti-c-Myb (phospho S11) antibody [EP769Y] (AB45150)
  • WB

Lab

Western blot - Anti-c-Myb (phospho S11) antibody [EP769Y] (AB45150)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-c-Myb (phospho S11) antibody [EP769Y] (ab45150) at 1/5000 dilution

Lane 1:

Untreated HL-60 (Human promyelocytic leukemia cell line) cell lysate at 10 µg

Lane 2:

HL-60 cell lysate, membrane treated with alkaline phosphatase at 10 µg

Secondary

All lanes:

HRP goat anti-rabbit IgG (H+L) at 1/2000 dilution

Predicted band size: 72 kDa

Observed band size: 77 kDa

false

Exposure time: 3min

Western blot - Anti-c-Myb (phospho S11) antibody [EP769Y] (AB45150)
  • WB

Unknown

Western blot - Anti-c-Myb (phospho S11) antibody [EP769Y] (AB45150)

All lanes:

Western blot - Anti-c-Myb (phospho S11) antibody [EP769Y] (ab45150) at 1/10000 dilution

All lanes:

Ramos (Human Burkitt's lymphoma cell line) membrane lysate

Predicted band size: 72 kDa

Observed band size: 72 kDa,75 kDa

false

Dot Blot - Anti-c-Myb (phospho S11) antibody [EP769Y] (AB45150)
  • Dot

Unknown

Dot Blot - Anti-c-Myb (phospho S11) antibody [EP769Y] (AB45150)

Dot blot analysis of c-Myb (phospho S11/12) peptide (Lane 1) and c-Myb unmodified peptide (Lane 2) labeling c-Myb (phospho S11) with purified ab45150 at a dilution of 1/1000. ab97051 (Peroxidase conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at a dilution of 1/2500.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : 3 minutes.

  • Carrier free

    Anti-c-Myb (phospho S11) antibody [EP769Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP769Y

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

Dot, IP, WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Alternative Products

Primary Antibodies

AB177510

Anti-c-Myb antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myb antibody (AB177510)

  • 0
  • 0
Primary Antibodies

AB169111

Anti-c-Myb antibody [ANA236B]

KO Validated

Western blot - Anti-c-Myb antibody [ANA236B] (AB169111)

  • 0
  • 0
Primary Antibodies

AB109127

Anti-c-Myb antibody [EPR718(2)]

RabMAb|Recombinant|KO Validated

Western blot - Anti-c-Myb antibody [EPR718(2)] (AB109127)

  • 0
  • 0
Primary Antibodies

AB239947

Anti-c-Myb antibody [EPR718(2)] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Western blot - Anti-c-Myb antibody [EPR718(2)] - BSA and Azide free (AB239947)

  • 0
  • 0
Primary Antibodies

AB117635

Anti-c-Myb antibody

Immunocytochemistry/ Immunofluorescence - Anti-c-Myb antibody (AB117635)

  • 5
  • 1
Primary Antibodies

AB226470

Anti-c-Myb antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myb antibody (AB226470)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB110641

Anti-BRG1 antibody [EPNCIR111A]

BOND RX™ Validated|Recombinant|KO Validated|Advanced Validation|RabMAb

ChIC/CUT&RUN sequencing - Anti-BRG1 antibody [EPNCIR111A] (AB110641)

  • 5
  • 10
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB4729

Anti-Histone H3 (acetyl K27) antibody - ChIP Grade

BOND RX™ Validated

ChIP - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (AB4729)

  • 5
  • 85
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/50", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" } } }
style
left-column

Product details

Product Specifications
Anti-c-Myb (phospho S11) antibody [EP769Y] (ab45150) is a rabbit recombinant monoclonal antibody and is validated for use in Dot, IHC-P, IP, WB in human samples.
Anti-c-Myb (phospho S11) antibody [EP769Y] (ab45150) specifically detects c-Myb Phospho-S11 (UniProt ID: P10242; Molecular weight: 72kDa) and is sold in 100 µL and 1 mL selling sizes.

Quality and Validation
Abcam's high quality manufacturing and validation processes ensure Anti-c-Myb (phospho S11) antibody [EP769Y] (ab45150) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-c-Myb (phospho S11) antibody [EP769Y] (ab45150) has been cited over 60 times in peer reviewed journals and is trusted by the scientific community.

Related Products
Conjugation-ready, carrier free format available for antibody clone EP769Y - ab217945.
Antibody clone EP769Y is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 647, Alexa Fluor® 488, Alexa Fluor® 594 (ab196932, ab197507, ab207025).

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The c-Myb protein also known as Myb MYB or c-myb acts mechanically as a transcription factor that regulates the expression of target genes. It plays a role in cellular processes like proliferation differentiation and apoptosis by binding to specific DNA sequences. c-Myb has a molecular mass of roughly 75 kDa. It predominantly expresses in hematopoietic cells where it significantly influences gene transcription activity. Also it appears in some epithelial tissues indicating its versatile role in different cell types.
Biological function summary

The protein serves as a master regulator in the development of various cell types. It is not part of a traditional complex but interacts with other proteins to exert its function such as binding with co-activators or repressors to modulate gene expression. c-Myb controls the transcription of genes that are critical for cell cycle regulation and differentiation especially in the context of blood cell lineages. The protein is particularly influential in the production of red blood cells and white blood cells.

Pathways

C-Myb fits integrally into known hematopoietic signaling cascades and the Wnt signaling pathway. It works alongside other proteins like cyclin-dependent kinase (CDK) family members and transcription factor GATA-1 to modulate lineage-specific gene expression. Through its regulatory actions c-Myb influences the activation of downstream effectors that propagate signals essential for maintaining cellular functions within these pathways. The modulation of these pathways underlines its role in cell fate decisions.

Studies link c-Myb to several forms of leukemia and colon cancer. In leukemia aberrant expression of c-Myb disrupts normal hematopoiesis contributing to the uncontrolled proliferation of immature blood cells. The abnormal interactions between c-Myb and proteins like Bcl-2 can lead to enhanced cellular survival further promoting malignant conditions. In colon cancer c-Myb overexpression influences tumor growth and progression often acting alongside other oncogenic factors to enhance disease severity.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Transcriptional activator; DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'. Plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells.
See full target information MYB phospho S11
style
left-column

Publications (68)

Recent publications for all applications. Explore the full list and refine your search

Acta biochimica et biophysica Sinica 57:1457-1468 PubMed39757769

2025

MYB represses ζ-globin expression through upregulating ETO2.

Applications

Unspecified application

Species

Unspecified reactive species

Zejun Dong,Yuhua Ye,Wei Zhang,Hualei Luo,Jialong Li,Qianqian Zhang,Xinhua Zhang,Xiang Guo,Xiangmin Xu

JCI insight 9: PubMed39499902

2024

BPDCN MYB fusions regulate cell cycle genes, impair differentiation, and induce myeloid-dendritic cell leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Christopher A G Booth,Juliette M Bouyssou,Katsuhiro Togami,Olivier Armand,Hembly G Rivas,Kezhi Yan,Siobhan Rice,Shuyuan Cheng,Emily M Lachtara,Jean-Pierre Bourquin,Alex Kentsis,Esther Rheinbay,James A DeCaprio,Andrew A Lane

Clinical cancer research : an official journal of the American Association for Cancer Research 30:5281-5292 PubMed39352719

2024

Phase 2 Trial of Regorafenib in Recurrent/Metastatic Adenoid Cystic Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Antoine Desilets,Joris L Vos,Nora Katabi,Fengshen Kuo,Zaineb Nadeem,Maximilian Linxweiler,Irina Ostrovnaya,Shrujal Baxi,Lara A Dunn,Eric J Sherman,David G Pfister,Luc G T Morris,Alan L Ho

Head and neck pathology 18:51 PubMed38896376

2024

A Novel Scoring System for MYB RNA In Situ Hybridization Displays High Sensitivity and Specificity for Adenoid Cystic Carcinoma in a Clinical Setting.

Applications

Unspecified application

Species

Unspecified reactive species

Mariel Bedell,Dale W Lewis,Raja R Seethala

NAR cancer 6:zcae020 PubMed38720882

2024

Integrated enhancer regulatory network by enhancer-promoter looping in gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Tianhui Zhu,Atsushi Okabe,Genki Usui,Ryoji Fujiki,Daichi Komiyama,Kie Kyon Huang,Motoaki Seki,Masaki Fukuyo,Hiroyuki Abe,Meng Ning,Tomoka Okada,Mizuki Minami,Makoto Matsumoto,Qin Fan,Bahityar Rahmutulla,Takayuki Hoshii,Patrick Tan,Teppei Morikawa,Tetsuo Ushiku,Atsushi Kaneda

Cancer 130:702-712 PubMed37947157

2023

A phase 2 study of MK-2206 in patients with incurable adenoid cystic carcinoma (Alliance A091104).

Applications

Unspecified application

Species

Unspecified reactive species

Alan L Ho,Nathan R Foster,Shyamprasad Deraje Vasudeva,Nora Katabi,Cristina R Antonescu,Gary P Frenette,David G Pfister,Charles Erlichman,Gary K Schwartz

iScience 26:107641 PubMed37670779

2023

Efficient genome editing in erythroid cells unveils novel MYB target genes and regulatory functions.

Applications

Unspecified application

Species

Unspecified reactive species

Virginie Deleuze,Leonor Garcia,Betty Rouaisnel,Mohammad Salma,Alexia Kinoo,Charlotte Andrieu-Soler,Eric Soler

Haematologica 108:1259-1271 PubMed36632736

2023

TAL1 activation in T-cell acute lymphoblastic leukemia: a novel oncogenic 3' neo-enhancer.

Applications

Unspecified application

Species

Unspecified reactive species

Charlotte Smith,Ashish Goyal,Dieter Weichenhan,Eric Allemand,Anand Mayakonda,Umut Toprak,Anna Riedel,Estelle Balducci,Manisha Manojkumar,Anastasija Pejkovska,Oliver Mücke,Etienne Sollier,Ali Bakr,Kersten Breuer,Pavlo Lutsik,Olivier Hermine,Salvatore Spicuglia,Vahid Asnafi,Christoph Plass,Aurore Touzart

Blood 142:336-351 PubMed36947815

2023

Structural variation cooperates with permissive chromatin to control enhancer hijacking-mediated oncogenic transcription.

Applications

Unspecified application

Species

Unspecified reactive species

Giovanni A Botten,Yuannyu Zhang,Kseniia Dudnyk,Yoon Jung Kim,Xin Liu,Jacob T Sanders,Aygun Imanci,Nathalie Droin,Hui Cao,Pranita Kaphle,Kathryn E Dickerson,Kirthi R Kumar,Mingyi Chen,Weina Chen,Eric Solary,Peter Ly,Jian Zhou,Jian Xu

Cell reports 41:111743 PubMed36450256

2022

Single-cell RNA sequencing identifies a paracrine interaction that may drive oncogenic notch signaling in human adenoid cystic carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Anuraag S Parikh,Avishai Wizel,Daniel Davis,Armida Lefranc-Torres,Alejandro I Rodarte-Rascon,Lauren E Miller,Kevin S Emerick,Mark A Varvares,Daniel G Deschler,William C Faquin,Jon C Aster,Derrick T Lin,Bradley E Bernstein,Yotam Drier,Sidharth V Puram
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com