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AB217945

Anti-c-Myb (phospho S11) antibody [EP769Y] - BSA and Azide free

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(13 Publications)

Rabbit Recombinant Monoclonal c-Myb phospho S11 antibody. Carrier free. Suitable for IHC-P, IP, Dot, WB and reacts with Human samples. Cited in 13 publications.

View Alternative Names

Transcriptional activator Myb, Proto-oncogene c-Myb, MYB

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myb (phospho S11) antibody [EP769Y] - BSA and Azide free (AB217945)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myb (phospho S11) antibody [EP769Y] - BSA and Azide free (AB217945)

Unpurified ab45150 staining human v-Myb / c-Myb in human cervical carcinoma by immunohistochemistry using paraffin embedded tissue.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab45150).

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunoprecipitation - Anti-c-Myb (phospho S11) antibody [EP769Y] - BSA and Azide free (AB217945)
  • IP

Lab

Immunoprecipitation - Anti-c-Myb (phospho S11) antibody [EP769Y] - BSA and Azide free (AB217945)

This data was developed using ab45150, the same antibody clone in a different buffer formulation.c-Myb was immunoprecipitated from 0.35 mg MOLT-4 (Human lymphoblastic leukemia T lymphoblast) whole cell lysate 10 µg with ab45150 at 1/120 dilution (2µg). VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : MOLT-4 (Human lymphoblastic leukemia T lymphoblast) whole cell lysate 10 µg

Lane 2 : abab45150 IP in MOLT-4 whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab45150 in MOLT-4 whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Fresh lysate should be used to minimize protein degradation. Multi-bands should be c-myb isoforms based on immunogen design and Uniprot database.

All lanes:

Immunoprecipitation - Anti-c-Myb (phospho S11) antibody [EP769Y] (<a href='/en-us/products/primary-antibodies/c-myb-phospho-s11-antibody-ep769y-ab45150'>ab45150</a>)

Predicted band size: 72 kDa

Observed band size: 75-77 kDa

false

Dot Blot - Anti-c-Myb (phospho S11) antibody [EP769Y] - BSA and Azide free (AB217945)
  • Dot

Unknown

Dot Blot - Anti-c-Myb (phospho S11) antibody [EP769Y] - BSA and Azide free (AB217945)

Dot blot analysis of v-Myb + c-Myb (phospho S11/12) peptide (Lane 1) and v-Myb + c-Myb unmodified peptide (Lane 2) labeling v-Myb + c-Myb (phospho S11) with purified ab45150 at a dilution of 1/1000. ab97051 (Peroxidase conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at a dilution of 1/2500.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : 3 minutes.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab45150).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP769Y

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IHC-P, Dot, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab217945 is the carrier-free version of ab45150.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The c-Myb protein also known as Myb MYB or c-myb acts mechanically as a transcription factor that regulates the expression of target genes. It plays a role in cellular processes like proliferation differentiation and apoptosis by binding to specific DNA sequences. c-Myb has a molecular mass of roughly 75 kDa. It predominantly expresses in hematopoietic cells where it significantly influences gene transcription activity. Also it appears in some epithelial tissues indicating its versatile role in different cell types.
Biological function summary

The protein serves as a master regulator in the development of various cell types. It is not part of a traditional complex but interacts with other proteins to exert its function such as binding with co-activators or repressors to modulate gene expression. c-Myb controls the transcription of genes that are critical for cell cycle regulation and differentiation especially in the context of blood cell lineages. The protein is particularly influential in the production of red blood cells and white blood cells.

Pathways

C-Myb fits integrally into known hematopoietic signaling cascades and the Wnt signaling pathway. It works alongside other proteins like cyclin-dependent kinase (CDK) family members and transcription factor GATA-1 to modulate lineage-specific gene expression. Through its regulatory actions c-Myb influences the activation of downstream effectors that propagate signals essential for maintaining cellular functions within these pathways. The modulation of these pathways underlines its role in cell fate decisions.

Studies link c-Myb to several forms of leukemia and colon cancer. In leukemia aberrant expression of c-Myb disrupts normal hematopoiesis contributing to the uncontrolled proliferation of immature blood cells. The abnormal interactions between c-Myb and proteins like Bcl-2 can lead to enhanced cellular survival further promoting malignant conditions. In colon cancer c-Myb overexpression influences tumor growth and progression often acting alongside other oncogenic factors to enhance disease severity.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcriptional activator; DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'. Plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells.
See full target information MYB phospho S11
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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

American journal of cancer research 11:773-792 PubMed33791153

2021

Establishment of patient-derived xenograft models of adenoid cystic carcinoma to assess pre-clinical efficacy of combination therapy of a PI3K inhibitor and retinoic acid.

Applications

Unspecified application

Species

Unspecified reactive species

Bao Sun,Yu Wang,Jingjing Sun,Chunye Zhang,Ronghui Xia,Shengming Xu,Shuyang Sun,Jiang Li

American journal of clinical pathology 146:741-746 PubMed27614667

2016

Fine-Needle Aspiration of Epithelial-Myoepithelial Carcinoma of the Parotid Gland With Prominent Adenoid Cystic Carcinoma-Like Cribriform Features: Avoiding a Diagnostic Pitfall.

Applications

Unspecified application

Species

Unspecified reactive species

Omonigho A Aisagbonhi,Mark A Tulecke,David C Wilbur,Atoussa Goldar-Najafi,Shams Iqbal,Peter M Sadow,William C Faquin

Science signaling 7:ra47 PubMed24847116

2014

Targeting poly(ADP-ribose) polymerase and the c-Myb-regulated DNA damage response pathway in castration-resistant prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Likun Li,Wenjun Chang,Guang Yang,Chengzhen Ren,Sanghee Park,Theodoros Karantanos,Styliani Karanika,Jianxiang Wang,Jianhua Yin,Parantu K Shah,Hirayama Takahiro,Masato Dobashi,Wenling Zhang,Eleni Efstathiou,Sankar N Maity,Ana M Aparicio,Elsa M Li Ning Tapia,Patricia Troncoso,Bradley Broom,Lianchun Xiao,Hyun-Sung Lee,Ju-Seog Lee,Paul G Corn,Nora Navone,Timothy C Thompson

Laboratory investigation; a journal of technical methods and pathology 94:692-702 PubMed24732452

2014

Analysis of MYB oncogene in transformed adenoid cystic carcinomas reveals distinct pathways of tumor progression.

Applications

Unspecified application

Species

Unspecified reactive species

Ana F Costa,Albina Altemani,Cristina García-Inclán,Florentino Fresno,Carlos Suárez,José L Llorente,Mario Hermsen

Oncogene 33:3947-58 PubMed24013223

2013

Mutations in 3'-long terminal repeat of HERV-W family in chromosome 7 upregulate syncytin-1 expression in urothelial cell carcinoma of the bladder through interacting with c-Myb.

Applications

Unspecified application

Species

Unspecified reactive species

H Yu,T Liu,Z Zhao,Y Chen,J Zeng,S Liu,F Zhu

American journal of ophthalmology 156:1147-1158.e1 PubMed23972314

2013

Immunohistochemical features of lacrimal gland epithelial tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Pia R Mendoza,Frederick A Jakobiec,Jeffrey F Krane

PloS one 8:e57958 PubMed23483952

2013

Allelic variation of the MMP3 promoter affects transcription activity through the transcription factor C-MYB in human brain arteriovenous malformations.

Applications

Unspecified application

Species

Unspecified reactive species

Cong Huai,Jianping Song,Zengyi Ma,Xuanfeng Qin,Peiliang Li,Hongyan Chen,Fan Zhao,Daru Lu,Donglei Song,Ying Mao,Xiao Song,Yao Zhao

Modern pathology : an official journal of the Unit 24:1169-76 PubMed21572406

2011

Analysis of MYB expression and MYB-NFIB gene fusions in adenoid cystic carcinoma and other salivary neoplasms.

Applications

Unspecified application

Species

Unspecified reactive species

Louis B Brill,William A Kanner,André Fehr,Ywonne Andrén,Christopher A Moskaluk,Thomas Löning,Göran Stenman,Henry F Frierson

Blood 117:3816-25 PubMed21296997

2011

MYB transcriptionally regulates the miR-155 host gene in chronic lymphocytic leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Karin Vargova,Nikola Curik,Pavel Burda,Petra Basova,Vojtech Kulvait,Vit Pospisil,Filipp Savvulidi,Juraj Kokavec,Emanuel Necas,Adela Berkova,Petra Obrtlikova,Josef Karban,Marek Mraz,Sarka Pospisilova,Jiri Mayer,Marek Trneny,Jiri Zavadil,Tomas Stopka

PloS one 5:e13073 PubMed20949095

2010

Potential tumor suppressor role for the c-Myb oncogene in luminal breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Aaron R Thorner,Joel S Parker,Katherine A Hoadley,Charles M Perou
View all publications
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