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AB316922

Anti c-Myc antibody

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Goat Polyclonal C-Myc/MYC antibody. Suitable for ICC/IF and reacts with Tag samples. Immunogen corresponding to Synthetic Peptide within Human MYC conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

BHLHE39, MYC, Myc proto-oncogene protein, Class E basic helix-loop-helix protein 39, Proto-oncogene c-Myc, Transcription factor p64, bHLHe39

3 Images
Immunocytochemistry/ Immunofluorescence - Anti c-Myc Antibody (AB316922)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti c-Myc Antibody (AB316922)

Immunofluorescent analysis of 4% paraformaldehyde (PFA)-fixed, 0.1% Triton X-100 permeabilized CHO cells transfected with GFP-MYC constructs (named as CHO-GFP-MYC, Top) and parental CHO cells (Bottom) labeling c-Myc with ab316922 at 1.5 ug/ml, followed by Donkey Anti-Goat IgG (H&L) Alexa-Fluor594® conjugated secondary antibody at 3 ug/ml) (Red). Nucleus was counterstained with DAPI (Blue).

Immunocytochemistry/ Immunofluorescence - Anti c-Myc Antibody (AB316922)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti c-Myc Antibody (AB316922)

Immunofluorescent analysis of 4% paraformaldehyde (PFA)-fixed, 0.1% Triton X-100 permeabilized CHO cells transfected with 12 tags constructs (CHO-12 tags) labeling c-Myc with ab316922 at 4 ug/ml, followed by Donkey Anti-Goat IgG (H&L) FITC conjugated secondary antibody at a 1/1000 dilution (1 ug/ml) (Green). Nucleus was counterstained with DAPI (White).

Immunocytochemistry/ Immunofluorescence - Anti c-Myc Antibody (AB316922)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti c-Myc Antibody (AB316922)

Parallel staining with ab316922 and Donkey Anti-Goat IgG (H&L) FITC conjugated secondary antibody in negative control parental CHO cell line.

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Applications

ICC/IF

applications

Immunogen

Synthetic Peptide within Human MYC conjugated to Keyhole Limpet Haemocyanin.

P01106

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Complementary Products

Assay Kits

AB277452

Human c-Myc ELISA Kit

ELISA - Human c-Myc ELISA Kit (AB277452)

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Primary Antibodies

AB236632

Anti-HA tag antibody [EPR22819-101]

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HA tag antibody [EPR22819-101] (AB236632)

  • 3
  • 1
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Primary Antibodies

AB183734

Anti-GFP antibody [EPR14104]

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104] (AB183734)

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Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1.5-4 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.09% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

C-Myc also known simply as Myc or MYC protein is a transcription factor with significant roles in cellular processes. Its estimated molecular weight is approximately 62 kDa. This protein is expressed in various tissues and cell types including common use in HEK (human embryonic kidney) cells for research. c-Myc functions by regulating transcription of genes involved in cell cycle progression apoptosis and cellular transformation.
Biological function summary

C-Myc is involved in regulating cell growth and proliferation. It forms part of the Myc/Max complex which binds to DNA to regulate gene expression. This activity affects cellular metabolism ribosome biogenesis and cell cycle entry emphasizing its regulation of cellular energy and stress response. Its expression levels critically govern normal cellular functions and homeostasis.

Pathways

C-Myc plays an important role in the cell cycle pathway and apoptosis regulation. Specifically c-Myc is associated with the Wnt signaling pathway which influences cellular proliferation and differentiation. It also interacts with other proteins like Cyclin D1 influencing cell cycle control. These interactions ensure c-Myc's involvement in regulating key processes related to cell proliferation and stability.

C-Myc is tightly linked to cancer such as Burkitt's lymphoma and colon cancer. In these conditions c-Myc overexpression contributes to uncontrolled cell proliferation. Additionally c-Myc is associated with other oncogenic proteins like BCL2 in tumorigenesis highlighting its pivotal role in cancer development and progression. Understanding c-Myc's involvement in these diseases aids in the development of targeted therapeutic strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3' (PubMed : 24940000, PubMed : 25956029). Activates the transcription of growth-related genes (PubMed : 24940000, PubMed : 25956029). Binds to the VEGFA promoter, promoting VEGFA production and subsequent sprouting angiogenesis (PubMed : 24940000, PubMed : 25956029). Regulator of somatic reprogramming, controls self-renewal of embryonic stem cells (By similarity). Functions with TAF6L to activate target gene expression through RNA polymerase II pause release (By similarity). Positively regulates transcription of HNRNPA1, HNRNPA2 and PTBP1 which in turn regulate splicing of pyruvate kinase PKM by binding repressively to sequences flanking PKM exon 9, inhibiting exon 9 inclusion and resulting in exon 10 inclusion and production of the PKM M2 isoform (PubMed : 20010808).
See full target information MYC
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Product promise

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