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AB51156

Anti-c-Myc (phospho S62) antibody

5

(1 Review)

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(43 Publications)

Rabbit Polyclonal C-Myc/MYC phospho S62 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 43 publications. Immunogen corresponding to Synthetic Peptide within Human MYC phospho S62.

View Alternative Names

BHLHE39, MYC, Myc proto-oncogene protein, Class E basic helix-loop-helix protein 39, Proto-oncogene c-Myc, Transcription factor p64, bHLHe39

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-c-Myc (phospho S62) antibody (AB51156)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-c-Myc (phospho S62) antibody (AB51156)

ICC/IF image of ab51156 stained HepG2 cells. The cells were 4% formlaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51156, 5μg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, a goat anti-rabbit DyLight® 488 (IgG; H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myc (phospho S62) antibody (AB51156)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myc (phospho S62) antibody (AB51156)

Immunohistochemical analysis of c-Myc (phospho S62) expression in paraffin-embedded human breast carcinoma tissue using 1/50 ab51156. left : untreated sample. Right : sample treated with phosphopeptide.

Immunocytochemistry/ Immunofluorescence - Anti-c-Myc (phospho S62) antibody (AB51156)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-c-Myc (phospho S62) antibody (AB51156)

Immunofluorescence analysis of HeLa cells, treated (left) and untreated (right) with Forskolin (40nM, 15mins), using c-Myc (phospho-Ser62) antibody.

Western blot - Anti-c-Myc (phospho S62) antibody (AB51156)
  • WB

Unknown

Western blot - Anti-c-Myc (phospho S62) antibody (AB51156)

All lanes:

Western blot - Anti-c-Myc (phospho S62) antibody (ab51156) at 1/500 dilution

Lane 1:

extracts from 293 cells treatedwith 40nM Forskolin for 30min, with phosphopeptide

Lane 2:

extracts from 293 cells treated with 40nM Forskolin for 30min, without phosphopeptide

Predicted band size: 48 kDa

Observed band size: 49 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human MYC phospho S62. The exact immunogen used to generate this antibody is proprietary information.

P01106

Specificity

ab51156 detects endogenous levels of Myc only when phosphorylated at serine 62.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Storage buffer
pH: 7 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

C-Myc also known simply as Myc or MYC protein is a transcription factor with significant roles in cellular processes. Its estimated molecular weight is approximately 62 kDa. This protein is expressed in various tissues and cell types including common use in HEK (human embryonic kidney) cells for research. c-Myc functions by regulating transcription of genes involved in cell cycle progression apoptosis and cellular transformation.
Biological function summary

C-Myc is involved in regulating cell growth and proliferation. It forms part of the Myc/Max complex which binds to DNA to regulate gene expression. This activity affects cellular metabolism ribosome biogenesis and cell cycle entry emphasizing its regulation of cellular energy and stress response. Its expression levels critically govern normal cellular functions and homeostasis.

Pathways

C-Myc plays an important role in the cell cycle pathway and apoptosis regulation. Specifically c-Myc is associated with the Wnt signaling pathway which influences cellular proliferation and differentiation. It also interacts with other proteins like Cyclin D1 influencing cell cycle control. These interactions ensure c-Myc's involvement in regulating key processes related to cell proliferation and stability.

C-Myc is tightly linked to cancer such as Burkitt's lymphoma and colon cancer. In these conditions c-Myc overexpression contributes to uncontrolled cell proliferation. Additionally c-Myc is associated with other oncogenic proteins like BCL2 in tumorigenesis highlighting its pivotal role in cancer development and progression. Understanding c-Myc's involvement in these diseases aids in the development of targeted therapeutic strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3' (PubMed : 24940000, PubMed : 25956029). Activates the transcription of growth-related genes (PubMed : 24940000, PubMed : 25956029). Binds to the VEGFA promoter, promoting VEGFA production and subsequent sprouting angiogenesis (PubMed : 24940000, PubMed : 25956029). Regulator of somatic reprogramming, controls self-renewal of embryonic stem cells (By similarity). Functions with TAF6L to activate target gene expression through RNA polymerase II pause release (By similarity). Positively regulates transcription of HNRNPA1, HNRNPA2 and PTBP1 which in turn regulate splicing of pyruvate kinase PKM by binding repressively to sequences flanking PKM exon 9, inhibiting exon 9 inclusion and resulting in exon 10 inclusion and production of the PKM M2 isoform (PubMed : 20010808).
See full target information MYC phospho S62
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Publications (43)

Recent publications for all applications. Explore the full list and refine your search

ACS central science 9:787-804 PubMed37122450

2023

Profiling Germinal Center-like B Cell Responses to Conjugate Vaccines Using Synthetic Immune Organoids.

Applications

Unspecified application

Species

Unspecified reactive species

Tyler D Moeller,Shivem B Shah,Kristine Lai,Natalia Lopez-Barbosa,Primit Desai,Weiyao Wang,Zhe Zhong,David Redmond,Ankur Singh,Matthew P DeLisa

Nature communications 14:762 PubMed36765089

2023

Loss of phosphatase CTDNEP1 potentiates aggressive medulloblastoma by triggering MYC amplification and genomic instability.

Applications

Unspecified application

Species

Unspecified reactive species

Zaili Luo,Dazhuan Xin,Yunfei Liao,Kalen Berry,Sean Ogurek,Feng Zhang,Liguo Zhang,Chuntao Zhao,Rohit Rao,Xinran Dong,Hao Li,Jianzhong Yu,Yifeng Lin,Guoying Huang,Lingli Xu,Mei Xin,Ryuichi Nishinakamura,Jiyang Yu,Marcel Kool,Stefan M Pfister,Martine F Roussel,Wenhao Zhou,William A Weiss,Paul Andreassen,Q Richard Lu

Molecular oncology 17:964-980 PubMed36650715

2023

Rational combination of SHP2 and mTOR inhibition for the treatment of hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Antonio Mulero-Sánchez,Christel F A Ramirez,Aimée du Chatinier,Hui Wang,Sofie J I Koomen,Ji-Ying Song,Marnix H P de Groot,Cor Lieftink,Astrid Bosma,Artur Burylo,Olaf van Tellingen,Roderick L Beijersbergen,Cun Wang,Leila Akkari,René Bernards,Sara Mainardi

NPJ precision oncology 6:68 PubMed36153348

2022

Co-targeting CDK2 and CDK4/6 overcomes resistance to aromatase and CDK4/6 inhibitors in ER+ breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Abeer J Al-Qasem,Carla L Alves,Sidse Ehmsen,Martina Tuttolomondo,Mikkel G Terp,Lene E Johansen,Henriette Vever,Luna V A Hoeg,Daniel Elias,Martin Bak,Henrik J Ditzel

Nature communications 13:5237 PubMed36068222

2022

PIM1 promotes hepatic conversion by suppressing reprogramming-induced ferroptosis and cell cycle arrest.

Applications

Unspecified application

Species

Unspecified reactive species

Yangyang Yuan,Chenwei Wang,Xuran Zhuang,Shaofeng Lin,Miaomiao Luo,Wankun Deng,Jiaqi Zhou,Lihui Liu,Lina Mao,Wenbo Peng,Jian Chen,Qiangsong Wang,Yilai Shu,Yu Xue,Pengyu Huang

iScience 25:103944 PubMed35535207

2022

A text-based computational framework for patient -specific modeling for classification of cancers.

Applications

Unspecified application

Species

Unspecified reactive species

Hiroaki Imoto,Sawa Yamashiro,Mariko Okada

Bone research 10:24 PubMed35232979

2022

Sialylation of TLR2 initiates osteoclast fusion.

Applications

Unspecified application

Species

Unspecified reactive species

Ce Dou,Gehua Zhen,Yang Dan,Mei Wan,Nathachit Limjunyawong,Xu Cao

Cell reports 37:109974 PubMed34758313

2021

Single-cell transcriptomics reveals opposing roles of Shp2 in Myc-driven liver tumor cells and microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Wendy S Chen,Yan Liang,Min Zong,Jacey J Liu,Kota Kaneko,Kaisa L Hanley,Kun Zhang,Gen-Sheng Feng

Molecular therapy. Nucleic acids 25:83-92 PubMed34258104

2021

Concurrent targeting of MAP3K3 and BRD4 by overcomes acquired resistance to BET inhibitors in neuroblastoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Chang Liu,Yasuyuki Gen,Kousuke Tanimoto,Tomoki Muramatsu,Jun Inoue,Johji Inazawa

Cell death & disease 12:396 PubMed33854043

2021

Directly targeting c-Myc contributes to the anti-multiple myeloma effect of anlotinib.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Cao,Huizhuang Shan,Meng Liu,Jia Liu,Zilu Zhang,Xiaoguang Xu,Yue Liu,Hanzhang Xu,Hu Lei,Miao Yu,Xingming Zhang,Wanting Liu,Zhilei Bu,Zhixiao Fang,Yanjie Ji,Hua Yan,Weiying Gu,Yingli Wu
View all publications
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