Rabbit Recombinant Monoclonal C-Myc/MYC phospho S62 antibody. Carrier free. Suitable for IP, Dot, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myc (phospho S62) antibody [EPR17924] - BSA and Azide free (AB232691), expandable thumbnail](https://content.abcam.com/products/images/c-myc-phospho-s62-antibody-epr17924-bsa-and-azide-free-ab232691--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img58792.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myc (phospho S62) antibody [EPR17924] - BSA and Azide free (AB232691)")
![Immunocytochemistry/ Immunofluorescence - Anti-c-Myc (phospho S62) antibody [EPR17924] - BSA and Azide free (AB232691), expandable thumbnail](https://content.abcam.com/products/images/c-myc-phospho-s62-antibody-epr17924-bsa-and-azide-free-ab232691--immunocytochemistry-immunofluorescence-img127345.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-c-Myc (phospho S62) antibody [EPR17924] - BSA and Azide free (AB232691)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myc (phospho S62) antibody [EPR17924] - BSA and Azide free (AB232691), expandable thumbnail](https://content.abcam.com/products/images/c-myc-phospho-s62-antibody-epr17924-bsa-and-azide-free-ab232691--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img3622.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myc (phospho S62) antibody [EPR17924] - BSA and Azide free (AB232691)")
![Flow Cytometry (Intracellular) - Anti-c-Myc (phospho S62) antibody [EPR17924] - BSA and Azide free (AB232691), expandable thumbnail](https://content.abcam.com/products/images/c-myc-phospho-s62-antibody-epr17924-bsa-and-azide-free-ab232691--flow-cytometry-intracellular-img25338.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-c-Myc (phospho S62) antibody [EPR17924] - BSA and Azide free (AB232691)")
![Immunoprecipitation - Anti-c-Myc (phospho S62) antibody [EPR17924] - BSA and Azide free (AB232691), expandable thumbnail](https://content.abcam.com/products/images/c-myc-phospho-s62-antibody-epr17924-bsa-and-azide-free-ab232691--immunoprecipitation-img60492.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-c-Myc (phospho S62) antibody [EPR17924] - BSA and Azide free (AB232691)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Dot | WB | ICC/IF | Flow Cyt (Intra) | IHC-P | |
|---|---|---|---|---|---|---|
| Human | Tested | Tested | Expected | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Expected | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Expected | Predicted | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes ab199376 - Rabbit monoclonal IgG (Low endotoxin, Azide free), is suitable for use as an isotype control with this antibody. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
8 products for Alternative Version
Target data
Function
Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Activates the transcription of growth-related genes. Binds to the VEGFA promoter, promoting VEGFA production and subsequent sprouting angiogenesis (PubMed:24940000). Regulator of somatic reprogramming, controls self-renewal of embryonic stem cells. Functions with TAF6L to activate target gene expression through RNA polymerase II pause release (By similarity).
Alternative names
Myc proto-oncogene protein, Class E basic helix-loop-helix protein 39, Proto-oncogene c-Myc, Transcription factor p64, bHLHe39, MYC, BHLHE39
Recommended products
Rabbit Recombinant Monoclonal C-Myc/MYC phospho S62 antibody. Carrier free. Suitable for IP, Dot, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples.
Alternative names
Myc proto-oncogene protein, Class E basic helix-loop-helix protein 39, Proto-oncogene c-Myc, Transcription factor p64, bHLHe39, MYC, BHLHE39
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR17924
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab232691 is the carrier-free version of Anti-c-Myc (phospho S62) antibody [EPR17924] ab185656.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
C-Myc also known simply as Myc or MYC protein is a transcription factor with significant roles in cellular processes. Its estimated molecular weight is approximately 62 kDa. This protein is expressed in various tissues and cell types including common use in HEK (human embryonic kidney) cells for research. c-Myc functions by regulating transcription of genes involved in cell cycle progression apoptosis and cellular transformation.
- Biological function summary
C-Myc is involved in regulating cell growth and proliferation. It forms part of the Myc/Max complex which binds to DNA to regulate gene expression. This activity affects cellular metabolism ribosome biogenesis and cell cycle entry emphasizing its regulation of cellular energy and stress response. Its expression levels critically govern normal cellular functions and homeostasis.
- Pathways
C-Myc plays an important role in the cell cycle pathway and apoptosis regulation. Specifically c-Myc is associated with the Wnt signaling pathway which influences cellular proliferation and differentiation. It also interacts with other proteins like Cyclin D1 influencing cell cycle control. These interactions ensure c-Myc's involvement in regulating key processes related to cell proliferation and stability.
- Associated diseases and disorders
C-Myc is tightly linked to cancer such as Burkitt's lymphoma and colon cancer. In these conditions c-Myc overexpression contributes to uncontrolled cell proliferation. Additionally c-Myc is associated with other oncogenic proteins like BCL2 in tumorigenesis highlighting its pivotal role in cancer development and progression. Understanding c-Myc's involvement in these diseases aids in the development of targeted therapeutic strategies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myc (phospho S62) antibody [EPR17924] - BSA and Azide free (ab232691), expandable thumbnail](https://content.abcam.com/products/images/c-myc-phospho-s62-antibody-epr17924-bsa-and-azide-free-ab232691--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img58792.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myc (phospho S62) antibody [EPR17924] - BSA and Azide free (ab232691)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myc (phospho S62) antibody [EPR17924] - BSA and Azide free (ab232691)
This IHC data was generated using the same anti-phospho S62 c-Myc antibody clone, EPR17924, in a different buffer formulation (cat# Anti-c-Myc (phospho S62) antibody [EPR17924] ab185656).
Immunohistochemical analysis of paraffin-embedded Human endometrium cancer tissue labeling c-Myc (phospho S62) with Anti-c-Myc (phospho S62) antibody [EPR17924] ab185656 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution.
Nuclear staining on cancer cells of Human endometrial cancer is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunocytochemistry/ Immunofluorescence - Anti-c-Myc (phospho S62) antibody [EPR17924] - BSA and Azide free (ab232691), expandable thumbnail](https://content.abcam.com/products/images/c-myc-phospho-s62-antibody-epr17924-bsa-and-azide-free-ab232691--immunocytochemistry-immunofluorescence-img127345.jpg "Immunocytochemistry/ Immunofluorescence - Anti-c-Myc (phospho S62) antibody [EPR17924] - BSA and Azide free (ab232691)")
Immunocytochemistry/ Immunofluorescence - Anti-c-Myc (phospho S62) antibody [EPR17924] - BSA and Azide free (ab232691)
This ICC/IF data was generated using the same anti-phospho S62 c-Myc antibody clone, EPR17924, in a different buffer formulation (cat# Anti-c-Myc (phospho S62) antibody [EPR17924] ab185656).
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling c-Myc (phospho S62) with Anti-c-Myc (phospho S62) antibody [EPR17924] ab185656 at 1/1000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/500 dilution (green).
Confocal image showing nuclear staining on HeLa cells.
The staining decreased after blocking with phospho peptide (100μg/ml) overnight.The control peptide is a non-phospho peptide.
The nuclear counter stain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows:
-ve control 1: Anti-c-Myc (phospho S62) antibody [EPR17924] ab185656 at 1/1000 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myc (phospho S62) antibody [EPR17924] - BSA and Azide free (ab232691), expandable thumbnail](https://content.abcam.com/products/images/c-myc-phospho-s62-antibody-epr17924-bsa-and-azide-free-ab232691--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img3622.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myc (phospho S62) antibody [EPR17924] - BSA and Azide free (ab232691)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myc (phospho S62) antibody [EPR17924] - BSA and Azide free (ab232691)
Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling c-Myc (phospho S62) with Anti-c-Myc (phospho S62) antibody [EPR17924] ab185656 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution.
Nuclear staining on rat testis is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-c-Myc (phospho S62) antibody [EPR17924] ab185656).
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Flow Cytometry (Intracellular) - Anti-c-Myc (phospho S62) antibody [EPR17924] - BSA and Azide free (ab232691), expandable thumbnail](https://content.abcam.com/products/images/c-myc-phospho-s62-antibody-epr17924-bsa-and-azide-free-ab232691--flow-cytometry-intracellular-img25338.jpg "Flow Cytometry (Intracellular) - Anti-c-Myc (phospho S62) antibody [EPR17924] - BSA and Azide free (ab232691)")
Flow Cytometry (Intracellular) - Anti-c-Myc (phospho S62) antibody [EPR17924] - BSA and Azide free (ab232691)
Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling c-Myc (phospho S62) with purified Anti-c-Myc (phospho S62) antibody [EPR17924] ab185656 at 1/150 dilution (red). The secondary antibody was Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution. A Rabbit monoclonal IgG (Black) was used as the isotype control and cells without incubation with primary antibody and secondary antibody (Blue) were used as unlabeled control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-c-Myc (phospho S62) antibody [EPR17924] ab185656).
![Immunoprecipitation - Anti-c-Myc (phospho S62) antibody [EPR17924] - BSA and Azide free (ab232691), expandable thumbnail](https://content.abcam.com/products/images/c-myc-phospho-s62-antibody-epr17924-bsa-and-azide-free-ab232691--immunoprecipitation-img60492.jpg "Immunoprecipitation - Anti-c-Myc (phospho S62) antibody [EPR17924] - BSA and Azide free (ab232691)")
Immunoprecipitation - Anti-c-Myc (phospho S62) antibody [EPR17924] - BSA and Azide free (ab232691)
c-Myc (phospho S62) was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate treated with 200nM TPA for 10 minutes with Anti-c-Myc (phospho S62) antibody [EPR17924] ab185656 at 1/50 dilution.
Western blot was performed from the immunoprecipitate using Anti-c-Myc (phospho S62) antibody [EPR17924] ab185656 at 1/1000 dilution.
VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used for detection at 1/1500 dilution.
Lane 1: HeLa whole cell lysate treated with 200nM TPA for 10 minutes,10 μg (Input).
Lane 2: Anti-c-Myc (phospho S62) antibody [EPR17924] ab185656 IP in HeLa whole cell lysate treated with 200nM TPA for 10 minutes.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-c-Myc (phospho S62) antibody [EPR17924] ab185656 in HeLa whole cell lysate treated with 200nM TPA for 10 minutes.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-c-Myc (phospho S62) antibody [EPR17924] ab185656).
All lanes: Immunoprecipitation - Anti-c-Myc (phospho S62) antibody [EPR17924] (Anti-c-Myc (phospho S62) antibody [EPR17924] ab185656)
Predicted band size: 48 kDa
Observed band size: 58 kDa
Exposure time: 5s
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myc (phospho S62) antibody [EPR17924] - BSA and Azide free (ab232691), expandable thumbnail](https://content.abcam.com/products/images/c-myc-phospho-s62-antibody-epr17924-bsa-and-azide-free-ab232691--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img123006.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myc (phospho S62) antibody [EPR17924] - BSA and Azide free (ab232691)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myc (phospho S62) antibody [EPR17924] - BSA and Azide free (ab232691)
Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling c-Myc (phospho S62) with Anti-c-Myc (phospho S62) antibody [EPR17924] ab185656 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution.
Nuclear and cytoplasmic staining on mouse spleen is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-c-Myc (phospho S62) antibody [EPR17924] ab185656).
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Dot Blot - Anti-c-Myc (phospho S62) antibody [EPR17924] - BSA and Azide free (ab232691), expandable thumbnail](https://content.abcam.com/products/images/c-myc-phospho-s62-antibody-epr17924-bsa-and-azide-free-ab232691--dot-blot-img33635.jpg "Dot Blot - Anti-c-Myc (phospho S62) antibody [EPR17924] - BSA and Azide free (ab232691)")
Dot Blot - Anti-c-Myc (phospho S62) antibody [EPR17924] - BSA and Azide free (ab232691)
Dot blot analysis of c -Myc (phospho T58) peptide (Lane 1), c-Myc non-phospho peptide (a control peptide for c-Myc phospho T58) (Lane 2), c-Myc (phospho S62) peptide (Lane 3), and c-Myc non-phospho peptide (a control peptide for c-Myc phospho S62) (Lane 4), labeled using Anti-c-Myc (phospho S62) antibody [EPR17924] ab185656 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/1000 dilution.
Blocking/Dilution buffer: 5% NFDM/TBST.
Lanes 1, 2 and 4 are control peptides, lane 3 contains the immunogen peptide.
Exposure time=3 minutes.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-c-Myc (phospho S62) antibody [EPR17924] ab185656).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myc (phospho S62) antibody [EPR17924] - BSA and Azide free (ab232691), expandable thumbnail](https://content.abcam.com/products/images/c-myc-phospho-s62-antibody-epr17924-bsa-and-azide-free-ab232691--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img123006.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myc (phospho S62) antibody [EPR17924] - BSA and Azide free (ab232691)")
![Dot Blot - Anti-c-Myc (phospho S62) antibody [EPR17924] - BSA and Azide free (ab232691), expandable thumbnail](https://content.abcam.com/products/images/c-myc-phospho-s62-antibody-epr17924-bsa-and-azide-free-ab232691--dot-blot-img33635.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-c-Myc (phospho S62) antibody [EPR17924] - BSA and Azide free (ab232691)")
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