Rabbit Polyclonal C-Myc/MYC phospho T58 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 29 publications. Immunogen corresponding to Synthetic Peptide within Human MYC phospho T58 aa 1-100.
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- Nature communications 14:63322023Signature-driven repurposing of Midostaurin for combination with MEK1/2 and KRASG12C inhibitors in lung cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIrati Macaya,Marta Roman,Connor Welch,Rodrigo Entrialgo-Cadierno,Marina Salmon,Alba Santos,Iker Feliu,Joanna Kovalski,Ines Lopez,Maria Rodriguez-Remirez,Sara Palomino-Echeverria,Shane M Lonfgren,Macarena Ferrero,Silvia Calabuig,Iziar A Ludwig,David Lara-Astiaso,Eloisa Jantus-Lewintre,Elizabeth Guruceaga,Shruthi Narayanan,Mariano Ponz-Sarvise,Antonio Pineda-Lucena,Fernando Lecanda,Davide Ruggero,Purvesh Khatri,Enrique Santamaria,Joaquin Fernandez-Irigoyen,Irene Ferrer,Luis Paz-Ares,Matthias Drosten,Mariano Barbacid,Ignacio Gil-Bazo,Silve VicentPubMed 37816716
- Clinical and translational medicine 13:e11532023Small molecule Z363 co-regulates TAF10 and MYC via the E3 ligase TRIP12 to suppress tumour growth.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYan Xiong,Lulu Wang,Shiyao Xu,Beibei Fu,Yuchen Che,Mohamed Y Zaky,Rong Tian,Rui Yao,Dong Guo,Zhou Sha,Feng Lin,Xiaoyuan Lin,Haibo WuPubMed 36639831
- British journal of haematology 198:338-3482022Suppression of MYC by PI3K/AKT/mTOR pathway inhibition in combination with all-trans retinoic acid treatment for therapeutic gain in acute myeloid leukaemia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSven Stengel,Kevin R Petrie,Yordan Sbirkov,Clara Stanko,Faezeh Ghazvini Zadegan,Veronica Gil,Rafał Skopek,Paweł Kamiński,Łukasz Szymański,Annamaria Brioli,Arthur Zelent,Tino SchenkPubMed 35468223
- PLoS genetics 17:e10093342021Cep55 regulation of PI3K/Akt signaling is required for neocortical development and ciliogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBehnam Rashidieh,Belal Shohayeb,Amanda Louise Bain,Patrick R J Fortuna,Debottam Sinha,Andrew Burgess,Richard Mills,Rachael C Adams,J Alejandro Lopez,Peter Blumbergs,John Finnie,Murugan Kalimutho,Michael Piper,James Edward Hudson,Dominic C H Ng,Kum Kum KhannaPubMed 34710087
- iScience 24:1029312021The BTK/PI3K/BRD4 axis inhibitor SRX3262 overcomes Ibrutinib resistance in mantle cell lymphoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDhananjaya Pal,Kendra R Vann,Shweta Joshi,Namood E Sahar,Guillermo A Morales,Dalia El-Gamal,Tatiana G Kutateladze,Donald L DurdenPubMed 34557659
- Cell reports 35:1092912021Targeting p130Cas- and microtubule-dependent MYC regulation sensitizes pancreatic cancer to ERK MAPK inhibition.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAndrew M Waters,Tala O Khatib,Bjoern Papke,Craig M Goodwin,G Aaron Hobbs,J Nathaniel Diehl,Runying Yang,A Cole Edwards,Katherine H Walsh,Rita Sulahian,James M McFarland,Kevin S Kapner,Thomas S K Gilbert,Clint A Stalnecker,Sehrish Javaid,Anna Barkovskaya,Kajal R Grover,Priya S Hibshman,Devon R Blake,Antje Schaefer,Katherine M Nowak,Jennifer E Klomp,Tikvah K Hayes,Michelle Kassner,Nanyun Tang,Olga Tanaseichuk,Kaisheng Chen,Yingyao Zhou,Manpreet Kalkat,Laura E Herring,Lee M Graves,Linda Z Penn,Hongwei H Yin,Andrew J Aguirre,William C Hahn,Adrienne D Cox,Channing J DerPubMed 34192548
- Cell death & disease 12:3962021Directly targeting c-Myc contributes to the anti-multiple myeloma effect of anlotinib.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYang Cao,Huizhuang Shan,Meng Liu,Jia Liu,Zilu Zhang,Xiaoguang Xu,Yue Liu,Hanzhang Xu,Hu Lei,Miao Yu,Xingming Zhang,Wanting Liu,Zhilei Bu,Zhixiao Fang,Yanjie Ji,Hua Yan,Weiying Gu,Yingli WuPubMed 33854043
- Advanced science (Weinheim, Baden-Wurttemberg, Germany) 8:20024972021Senescent Tumor Cells Build a Cytokine Shield in Colorectal Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYong Won Choi,Young Hwa Kim,Seung Yeop Oh,Kwang Wook Suh,Young-Sam Kim,Ga-Yeon Lee,Jung Eun Yoon,Soon Sang Park,Young-Kyoung Lee,Yoo Jung Park,Hong Seok Kim,So Hyun Park,Jang-Hee Kim,Tae Jun ParkPubMed 33643790
- Haematologica Online ahead of print:2020Transducin β-like protein 1 controls multiple oncogenic networks in diffuse large B-cell lymphoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYoussef Youssef et. al.PubMed 33054136
- Cancers 12:2020Inhibition of the Lysophosphatidylinositol Transporter ABCC1 Reduces Prostate Cancer Cell Growth and Sensitizes to Chemotherapy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAikaterini Emmanouilidi et. al.PubMed 32718079
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human MYC phospho T58 aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link P01106
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Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/1000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
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Target data
Function
Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3' (PubMed:24940000, PubMed:25956029). Activates the transcription of growth-related genes (PubMed:24940000, PubMed:25956029). Binds to the VEGFA promoter, promoting VEGFA production and subsequent sprouting angiogenesis (PubMed:24940000, PubMed:25956029). Regulator of somatic reprogramming, controls self-renewal of embryonic stem cells (By similarity). Functions with TAF6L to activate target gene expression through RNA polymerase II pause release (By similarity). Positively regulates transcription of HNRNPA1, HNRNPA2 and PTBP1 which in turn regulate splicing of pyruvate kinase PKM by binding repressively to sequences flanking PKM exon 9, inhibiting exon 9 inclusion and resulting in exon 10 inclusion and production of the PKM M2 isoform (PubMed:20010808).
Alternative names
BHLHE39, MYC, Myc proto-oncogene protein, Class E basic helix-loop-helix protein 39, Proto-oncogene c-Myc, Transcription factor p64, bHLHe39
Recommended products
Rabbit Polyclonal C-Myc/MYC phospho T58 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 29 publications. Immunogen corresponding to Synthetic Peptide within Human MYC phospho T58 aa 1-100.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human MYC phospho T58 aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link P01106
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
The antibody was affinity-purified from rabbit antiserum byaffinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
C-Myc also known simply as Myc or MYC protein is a transcription factor with significant roles in cellular processes. Its estimated molecular weight is approximately 62 kDa. This protein is expressed in various tissues and cell types including common use in HEK (human embryonic kidney) cells for research. c-Myc functions by regulating transcription of genes involved in cell cycle progression apoptosis and cellular transformation.
- Biological function summary
C-Myc is involved in regulating cell growth and proliferation. It forms part of the Myc/Max complex which binds to DNA to regulate gene expression. This activity affects cellular metabolism ribosome biogenesis and cell cycle entry emphasizing its regulation of cellular energy and stress response. Its expression levels critically govern normal cellular functions and homeostasis.
- Pathways
C-Myc plays an important role in the cell cycle pathway and apoptosis regulation. Specifically c-Myc is associated with the Wnt signaling pathway which influences cellular proliferation and differentiation. It also interacts with other proteins like Cyclin D1 influencing cell cycle control. These interactions ensure c-Myc's involvement in regulating key processes related to cell proliferation and stability.
- Associated diseases and disorders
C-Myc is tightly linked to cancer such as Burkitt's lymphoma and colon cancer. In these conditions c-Myc overexpression contributes to uncontrolled cell proliferation. Additionally c-Myc is associated with other oncogenic proteins like BCL2 in tumorigenesis highlighting its pivotal role in cancer development and progression. Understanding c-Myc's involvement in these diseases aids in the development of targeted therapeutic strategies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-c-Myc (phospho T58) antibody (ab28842)
All lanes: Western blot - Anti-c-Myc (phospho T58) antibody (ab28842) at 1/500 dilution
All lanes: human ovarian cancer cell lysate
Predicted band size: 48 kDa
Observed band size: 120 kDa, 51 kDa, 55 kDa, 86 kDa
Immunocytochemistry/ Immunofluorescence - Anti-c-Myc (phospho T58) antibody (ab28842)
ICC/IF image of ab28864 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28864, 1μg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myc (phospho T58) antibody (ab28842)
Immunohistochemical analysis of paraffin-embedded breast carcinoma. Left: Using Myc (Phospho-Thr58) Antibody; Right: The same antibody preincubated with synthesized phosphopeptide.
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