AB28842

Anti-c-Myc (phospho T58) antibody

Name: Anti-c-Myc (phospho T58) antibody (ab28842) | Abcam
Brand: Abcam Limited
SKU: AB28842
Price: 400 USD
Availability: InStock
Rating: 4 (3 reviews)

Lab Essentials
What is this?

(3 Reviews)

(34 Publications)

Rabbit Polyclonal C-Myc/MYC phospho T58 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 34 publications. Immunogen corresponding to Synthetic Peptide within Human MYC phospho T58 aa 1-100.

View Alternative Names

BHLHE39, MYC, Myc proto-oncogene protein, Class E basic helix-loop-helix protein 39, Proto-oncogene c-Myc, Transcription factor p64, bHLHe39

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myc (phospho T58) antibody (AB28842)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myc (phospho T58) antibody (AB28842)

Immunohistochemical analysis of paraffin-embedded breast carcinoma. Left : Using Myc (Phospho-Thr58) Antibody; Right : The same antibody preincubated with synthesized phosphopeptide.

Immunocytochemistry/ Immunofluorescence - Anti-c-Myc (phospho T58) antibody (AB28842)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-c-Myc (phospho T58) antibody (AB28842)

ICC/IF image of ab28864 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28864, 1μg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor^® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor^® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

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Western blot - Anti-c-Myc (phospho T58) antibody (AB28842)

All lanes:

Western blot - Anti-c-Myc (phospho T58) antibody (ab28842) at 1/500 dilution

All lanes:

human ovarian cancer cell lysate

Predicted band size: 48 kDa

Observed band size: 120 kDa,51 kDa,55 kDa,86 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Synthetic Peptide within Human MYC phospho T58 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

P01106

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

The antibody was affinity-purified from rabbit antiserum byaffinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

C-Myc also known simply as Myc or MYC protein is a transcription factor with significant roles in cellular processes. Its estimated molecular weight is approximately 62 kDa. This protein is expressed in various tissues and cell types including common use in HEK (human embryonic kidney) cells for research. c-Myc functions by regulating transcription of genes involved in cell cycle progression apoptosis and cellular transformation.

Biological function summary

C-Myc is involved in regulating cell growth and proliferation. It forms part of the Myc/Max complex which binds to DNA to regulate gene expression. This activity affects cellular metabolism ribosome biogenesis and cell cycle entry emphasizing its regulation of cellular energy and stress response. Its expression levels critically govern normal cellular functions and homeostasis.

Pathways

C-Myc plays an important role in the cell cycle pathway and apoptosis regulation. Specifically c-Myc is associated with the Wnt signaling pathway which influences cellular proliferation and differentiation. It also interacts with other proteins like Cyclin D1 influencing cell cycle control. These interactions ensure c-Myc's involvement in regulating key processes related to cell proliferation and stability.

C-Myc is tightly linked to cancer such as Burkitt's lymphoma and colon cancer. In these conditions c-Myc overexpression contributes to uncontrolled cell proliferation. Additionally c-Myc is associated with other oncogenic proteins like BCL2 in tumorigenesis highlighting its pivotal role in cancer development and progression. Understanding c-Myc's involvement in these diseases aids in the development of targeted therapeutic strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3' (PubMed : 24940000, PubMed : 25956029). Activates the transcription of growth-related genes (PubMed : 24940000, PubMed : 25956029). Binds to the VEGFA promoter, promoting VEGFA production and subsequent sprouting angiogenesis (PubMed : 24940000, PubMed : 25956029). Regulator of somatic reprogramming, controls self-renewal of embryonic stem cells (By similarity). Functions with TAF6L to activate target gene expression through RNA polymerase II pause release (By similarity). Positively regulates transcription of HNRNPA1, HNRNPA2 and PTBP1 which in turn regulate splicing of pyruvate kinase PKM by binding repressively to sequences flanking PKM exon 9, inhibiting exon 9 inclusion and resulting in exon 10 inclusion and production of the PKM M2 isoform (PubMed : 20010808).

See full target information MYC phospho T58

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Publications (34)

Recent publications for all applications. Explore the full list and refine your search

The EMBO journal 43:4922-4953 PubMed39284912

2024

Acidity suppresses CD8 + T-cell function by perturbing IL-2, mTORC1, and c-Myc signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Romain Vuillefroy de Silly,Laetitia Pericou,Bili Seijo,Isaac Crespo,Melita Irving

Nature communications 14:6332 PubMed37816716

2023

Signature-driven repurposing of Midostaurin for combination with MEK1/2 and KRASG12C inhibitors in lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Irati Macaya,Marta Roman,Connor Welch,Rodrigo Entrialgo-Cadierno,Marina Salmon,Alba Santos,Iker Feliu,Joanna Kovalski,Ines Lopez,Maria Rodriguez-Remirez,Sara Palomino-Echeverria,Shane M Lonfgren,Macarena Ferrero,Silvia Calabuig,Iziar A Ludwig,David Lara-Astiaso,Eloisa Jantus-Lewintre,Elizabeth Guruceaga,Shruthi Narayanan,Mariano Ponz-Sarvise,Antonio Pineda-Lucena,Fernando Lecanda,Davide Ruggero,Purvesh Khatri,Enrique Santamaria,Joaquin Fernandez-Irigoyen,Irene Ferrer,Luis Paz-Ares,Matthias Drosten,Mariano Barbacid,Ignacio Gil-Bazo,Silve Vicent

Clinical and translational medicine 13:e1153 PubMed36639831

2023

Small molecule Z363 co-regulates TAF10 and MYC via the E3 ligase TRIP12 to suppress tumour growth.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Xiong,Lulu Wang,Shiyao Xu,Beibei Fu,Yuchen Che,Mohamed Y Zaky,Rong Tian,Rui Yao,Dong Guo,Zhou Sha,Feng Lin,Xiaoyuan Lin,Haibo Wu

British journal of haematology 198:338-348 PubMed35468223

2022

Suppression of MYC by PI3K/AKT/mTOR pathway inhibition in combination with all-trans retinoic acid treatment for therapeutic gain in acute myeloid leukaemia.

Applications

Unspecified application

Species

Unspecified reactive species

Sven Stengel,Kevin R Petrie,Yordan Sbirkov,Clara Stanko,Faezeh Ghazvini Zadegan,Veronica Gil,Rafał Skopek,Paweł Kamiński,Łukasz Szymański,Annamaria Brioli,Arthur Zelent,Tino Schenk

Cell reports 37:109974 PubMed34758313

2021

Single-cell transcriptomics reveals opposing roles of Shp2 in Myc-driven liver tumor cells and microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Wendy S Chen,Yan Liang,Min Zong,Jacey J Liu,Kota Kaneko,Kaisa L Hanley,Kun Zhang,Gen-Sheng Feng

PLoS genetics 17:e1009334 PubMed34710087

2021

Cep55 regulation of PI3K/Akt signaling is required for neocortical development and ciliogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Behnam Rashidieh,Belal Shohayeb,Amanda Louise Bain,Patrick R J Fortuna,Debottam Sinha,Andrew Burgess,Richard Mills,Rachael C Adams,J Alejandro Lopez,Peter Blumbergs,John Finnie,Murugan Kalimutho,Michael Piper,James Edward Hudson,Dominic C H Ng,Kum Kum Khanna

iScience 24:102931 PubMed34557659

2021

The BTK/PI3K/BRD4 axis inhibitor SRX3262 overcomes Ibrutinib resistance in mantle cell lymphoma.

Applications

Unspecified application

Species

Unspecified reactive species

Dhananjaya Pal,Kendra R Vann,Shweta Joshi,Namood E Sahar,Guillermo A Morales,Dalia El-Gamal,Tatiana G Kutateladze,Donald L Durden

Cell reports 35:109291 PubMed34192548

2021

Targeting p130Cas- and microtubule-dependent MYC regulation sensitizes pancreatic cancer to ERK MAPK inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Andrew M Waters,Tala O Khatib,Bjoern Papke,Craig M Goodwin,G Aaron Hobbs,J Nathaniel Diehl,Runying Yang,A Cole Edwards,Katherine H Walsh,Rita Sulahian,James M McFarland,Kevin S Kapner,Thomas S K Gilbert,Clint A Stalnecker,Sehrish Javaid,Anna Barkovskaya,Kajal R Grover,Priya S Hibshman,Devon R Blake,Antje Schaefer,Katherine M Nowak,Jennifer E Klomp,Tikvah K Hayes,Michelle Kassner,Nanyun Tang,Olga Tanaseichuk,Kaisheng Chen,Yingyao Zhou,Manpreet Kalkat,Laura E Herring,Lee M Graves,Linda Z Penn,Hongwei H Yin,Andrew J Aguirre,William C Hahn,Adrienne D Cox,Channing J Der

Cell death & disease 12:396 PubMed33854043

2021

Directly targeting c-Myc contributes to the anti-multiple myeloma effect of anlotinib.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Cao,Huizhuang Shan,Meng Liu,Jia Liu,Zilu Zhang,Xiaoguang Xu,Yue Liu,Hanzhang Xu,Hu Lei,Miao Yu,Xingming Zhang,Wanting Liu,Zhilei Bu,Zhixiao Fang,Yanjie Ji,Hua Yan,Weiying Gu,Yingli Wu

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 8:2002497 PubMed33643790

2021

Senescent Tumor Cells Build a Cytokine Shield in Colorectal Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yong Won Choi,Young Hwa Kim,Seung Yeop Oh,Kwang Wook Suh,Young-Sam Kim,Ga-Yeon Lee,Jung Eun Yoon,Soon Sang Park,Young-Kyoung Lee,Yoo Jung Park,Hong Seok Kim,So Hyun Park,Jang-Hee Kim,Tae Jun Park

View all publications

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