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Rabbit Recombinant Monoclonal Insulin antibody. Suitable for IHC-P, Flow Cyt (Intra), I-ELISA, ICC/IF and reacts with Mouse, Rat, Recombinant fragment - Mouse, Recombinant fragment - Rat samples.

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Images

Indirect ELISA - Anti-C Peptide antibody [EPR25854-146] (AB314215), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-C Peptide antibody [EPR25854-146] (AB314215), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-C Peptide antibody [EPR25854-146] (AB314215), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-C Peptide antibody [EPR25854-146] (AB314215), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Peptide antibody [EPR25854-146] (AB314215), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PFlow Cyt (Intra)I-ELISAICC/IF
Human
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Tested
Tested
Expected
Tested
Rat
Tested
Tested
Expected
Expected
Recombinant fragment - Mouse
Not recommended
Not recommended
Tested
Not recommended
Recombinant fragment - Rat
Not recommended
Not recommended
Tested
Not recommended

Tested
Tested

Species
Mouse
Dilution info
1/500
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/2000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human, Recombinant fragment - Mouse, Recombinant fragment - Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/500
Notes

-

Species
Rat
Dilution info
1/50
Notes

-

Not recommended
Not recommended

Species
Human, Recombinant fragment - Mouse, Recombinant fragment - Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Recombinant fragment - Mouse
Dilution info
500 ng/mL
Notes

-

Species
Recombinant fragment - Rat
Dilution info
500 ng/mL
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/500
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Human, Recombinant fragment - Mouse, Recombinant fragment - Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.

Alternative names

Ins-1, Ins1, Insulin-1

Recommended products

Rabbit Recombinant Monoclonal Insulin antibody. Suitable for IHC-P, Flow Cyt (Intra), I-ELISA, ICC/IF and reacts with Mouse, Rat, Recombinant fragment - Mouse, Recombinant fragment - Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR25854-146
Purification technique
Affinity purification Protein A
Specificity

This antibody specifically recognizes the C peptide of both INS1 and INS2, but not proinsulin.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

C-peptide also known as connecting peptide is a short polypeptide chain consisting of 31 amino acids with a molecular weight of about 3 kDa. It results from enzymatic cleavage during the conversion of proinsulin to insulin and is co-secreted equimolarly with insulin by beta cells of the pancreas. C-peptide does not undergo significant liver metabolism making its levels a meaningful indicator of endogenous insulin production. This peptide is important due to its ability to serve as a biomarker for beta cell function giving insights into the body's insulin production capacity. Commercially available assays for its measurement include C-peptide ELISA and C-peptide ELISA kit important in both clinical and research settings.

Biological function summary

C-peptide plays a role beyond being a mere byproduct of insulin synthesis. Experiments have shown it binds to cell membranes indicating it functions independently and is more than part of the insulin processing complex. Studies suggest C-peptide may have physiological effects such as improving blood flow and possessing anti-inflammatory properties. Its ability to bind likely involves specific interactions that suggest possible receptors a subject of ongoing research. Detection methods like peptide ELISA and peptide test kit further facilitate studying its biological activity and potential therapeutic implications.

Pathways

C-peptide is closely linked to the metabolic insulin signaling pathway. This pathway is integral in glucose homeostasis and involves proteins such as insulin receptor and glucose transporter. Although not directly involved in the receptor-mediated actions of insulin C-peptide's presence alongside insulin shortly after release places it within this critical pathway. Another related pathway includes peptide hormone responses where C-peptide might influence vasodilation or cellular signaling revealing potential avenues for regulating metabolic conditions.

Associated diseases and disorders

C-peptide is most notably associated with diabetes mellitus specifically type 1 and type 2. Its measurement helps to distinguish between the types of diabetes as it reflects the pancreas's ability to produce insulin. Lower levels often correlate with type 1 diabetes due to autoimmune destruction of beta cells whereas type 2 diabetes may still show normal C-peptide levels as the disease progresses. C-peptide also contributes to diabetic complications through associations with proteins involved in vascular function suggesting its possible role in conditions like diabetic nephropathy and retinopathy.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Indirect ELISA - Anti-C Peptide antibody [EPR25854-146] (ab314215), expandable thumbnail

    Indirect ELISA - Anti-C Peptide antibody [EPR25854-146] (ab314215)

    Indirect ELISA analysis of ab314215 at 500-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1:2500 dilution.
    Antigen: Rat C-peptide of insulin-1,Rat C-peptide of Insulin-2,Mouse C-peptide of Insulin-1,Rat proinsulin-1, Mouse proinsulin-1.
    Antigen concentration: 100 ng/ml

  • Flow Cytometry (Intracellular) - Anti-C Peptide antibody [EPR25854-146] (ab314215), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-C Peptide antibody [EPR25854-146] (ab314215)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Isotype (Left) / AR42J (rat pancreatic tumor epithelial cell) treated with 100pM HGF and 2nM Activin A for 7days (Middle) / Untreated AR42J (Right) cells labelling C-Peptide with ab314215 at 1/50 dilution (1 ug)/Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody. PMID: 8756573. According to the literature, about 10% induced cells can express the insulin.

  • Flow Cytometry (Intracellular) - Anti-C Peptide antibody [EPR25854-146] (ab314215), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-C Peptide antibody [EPR25854-146] (ab314215)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (mouse embryonic fibroblast, Left) / Beta-TC-6 (mouse pancreas insulinoma beta cell, Right) cells labelling C-Peptide with ab314215 at 1/500 dilution (0.1 ug)/Red (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody. Negative control: NIH/3T3 (PMID: 23662125).

  • Immunocytochemistry/ Immunofluorescence - Anti-C Peptide antibody [EPR25854-146] (ab314215), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-C Peptide antibody [EPR25854-146] (ab314215)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Beta-TC-6 (mouse pancreas insulinoma beta cell) cells labelling C-Peptide with ab314215 at 1/500 (1.086 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing cytoplasmic staining in Beta-TC-6 cell line. Negative control: NIH/3T3 (PMID: 23662125). Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml)) dilution (Red). The Nuclear counterstain was DAPI (Blue).
    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Peptide antibody [EPR25854-146] (ab314215), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Peptide antibody [EPR25854-146] (ab314215)

    Immunohistochemical analysis of paraffin-embedded rat cardiac muscle tissue labeling C-Peptide with ab314215 at 1/2000 (0.272 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: no staining on rat cardiac muscle. The section was incubated with ab314215 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Peptide antibody [EPR25854-146] (ab314215), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Peptide antibody [EPR25854-146] (ab314215)

    Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue labeling C-Peptide with ab314215 at 1/500 (1.086 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: no staining on mouse cardiac muscle. The section was incubated with ab314215 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Peptide antibody [EPR25854-146] (ab314215), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Peptide antibody [EPR25854-146] (ab314215)

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue labeling C-Peptide with ab314215 at 1/2000 (0.272 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on islet of rat pancreas. The section was incubated with ab314215 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Peptide antibody [EPR25854-146] (ab314215), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Peptide antibody [EPR25854-146] (ab314215)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue labeling C-Peptide with ab314215 at 1/500 (1.086 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on islet of mouse pancreas. The section was incubated with ab314215 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

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Product protocols

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