Anti-C Peptide antibody [EPR25854-146]

• mIHC

Lab

Multiplex immunohistochemistry - Anti-C Peptide antibody [EPR25854-146] (AB314215)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse pancreas tissue staining Chymotrypsin with ab318203 at a 1/6000 dilution, ab317319 anti-Claudin-3 used at 1/2000 dilution and ab314215 anti-C Peptide used at a 1/500 dilution. Opal Polymer HRP Ms + Rb was used as a secondary antibody.

Panel A : merged staining of anti-Chymotrypsin (green; Opal^™520), anti-Claudin 3 (magenta; Opal^™690) and anti-C Peptide (gray; Opal^™570) on mouse pancreas.
Panel B : anti-Chymotrypsin staining exocrine gland in mouse pancreas.
Panel C : anti-Claudin 3 staining cell membrane in mouse pancreas.
Panel D : anti-C Peptide staining islet cells in mouse pancreas.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab318203, ab317319 and ab314215 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal^™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Peptide antibody [EPR25854-146] (AB314215)

Immunohistochemical analysis of paraffin-embedded rat cardiac muscle tissue labeling C-Peptide with ab314215 at 1/2000 (0.272 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : no staining on rat cardiac muscle. The section was incubated with ab314215 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Peptide antibody [EPR25854-146] (AB314215)

Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue labeling C-Peptide with ab314215 at 1/500 (1.086 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : no staining on mouse cardiac muscle. The section was incubated with ab314215 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Peptide antibody [EPR25854-146] (AB314215)

Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue labeling C-Peptide with ab314215 at 1/500 (1.086 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on islet of mouse pancreas. The section was incubated with ab314215 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Peptide antibody [EPR25854-146] (AB314215)

Immunohistochemical analysis of paraffin-embedded rat pancreas tissue labeling C-Peptide with ab314215 at 1/2000 (0.272 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on islet of rat pancreas. The section was incubated with ab314215 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-C Peptide antibody [EPR25854-146] (AB314215)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Beta-TC-6 (mouse pancreas insulinoma beta cell) cells labelling C-Peptide with ab314215 at 1/500 (1.086 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing cytoplasmic staining in Beta-TC-6 cell line. Negative control : NIH/3T3 (PMID : 23662125). Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml)) dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-C Peptide antibody [EPR25854-146] (AB314215)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Isotype (Left) / AR42J (rat pancreatic tumor epithelial cell) treated with 100pM HGF and 2nM Activin A for 7days (Middle) / Untreated AR42J (Right) cells labelling C-Peptide with ab314215 at 1/50 dilution (1 ug)/Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody. PMID : 8756573. According to the literature, about 10% induced cells can express the insulin.

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-C Peptide antibody [EPR25854-146] (AB314215)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (mouse embryonic fibroblast, Left) / Beta-TC-6 (mouse pancreas insulinoma beta cell, Right) cells labelling C-Peptide with ab314215 at 1/500 dilution (0.1 ug)/Red (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody. Negative control : NIH/3T3 (PMID : 23662125).

• mIHC

Lab

Multiplex immunohistochemistry - Anti-C Peptide antibody [EPR25854-146] (AB314215)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded ^® tissue staining C Peptide with ab314215 at a 1/500 dilution, ab318203 anti-Chymotrypsin used at 1/6000 dilution and ab52625 anti-Cytokeratin 19 used at a 1/6000 dilution.

Opal Polymer HRP Ms + Rb was used as a secondary antibody.

Panel A : merged staining of anti-C Peptide (green; Opal^™520), anti-Chymotrypsin (magenta; Opal^™690) and anti-Cytokeratin 19 (gray; Opal^™570) on rat pancreas.
Panel B : anti-C Peptide staining beta cells in rat pancreas.
Panel C : anti-Chymotrypsin staining exocrine gland in rat pancreas.
Panel D : anti-Cytokeratin 19 staining pancreatic duct in rat pancreas.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab314215, ab318203 and ab52625 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal^™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-C Peptide antibody [EPR25854-146] (AB314215)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded rat pancreas tissue staining C Peptide with ab314215 at a 1/500 dilution, ab278044 anti-Carboxypeptidase A used at 1/4000 dilution and ab52625 anti-Cytokeratin 19 used at a 1/6000 dilution.

Opal Polymer HRP Ms + Rb was used as a secondary antibody.

Panel A : merged staining of anti-C Peptide (green; Opal^™520), anti-Carboxypeptidase A (magenta; Opal^™690) and anti-Cytokeratin 19 (grey; Opal^™570) on rat pancreas.
Panel B : anti-C Peptide staining beta cells in rat pancreas.
Panel C : anti-Carboxypeptidase A staining exocrine gland in rat pancreas.
Panel D : anti-Cytokeratin 19 staining pancreatic duct in rat pancreas.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab314215, ab278044 and ab52625 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal^™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-C Peptide antibody [EPR25854-146] (AB314215)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded ^™ tissue staining C Peptide with ab314215 at a 1/500 dilution, ab318203 anti-Chymotrypsin used at 1/6000 dilution and ab52625 anti-Cytokeratin 19 used at a 1/6000 dilution.

Opal Polymer HRP Ms + Rb was used as a secondary antibody.

Panel A : merged staining of anti-C Peptide (green; Opal^™520), anti-Chymotrypsin (magenta; Opal^™690) and anti-Cytokeratin 19 (grey; Opal^™570) on mouse pancreas.
Panel B : anti-C Peptide staining beta cells in mouse pancreas.
Panel C : anti-Chymotrypsin staining exocrine gland in mouse pancreas.
Panel D : anti-Cytokeratin 19 staining pancreatic duct in mouse pancreas.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab314215, ab318203 and ab52625 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal^™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-C Peptide antibody [EPR25854-146] (AB314215)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded rat pancreas tissue staining Chymotrypsin with ab318203 at a 1/6000 dilution, ab317319 anti-Claudin-3 used at 1/2000 dilution and ab314215 anti-C Peptide used at a 1/500 dilution. Opal Polymer HRP Ms + Rb was used as a secondary antibody.

Panel A : merged staining of anti-Chymotrypsin (green; Opal^™520), anti-Claudin 3 (magenta; Opal^™690) and anti-C Peptide (gray; Opal^™570) on rat pancreas.
Panel B : anti-Chymotrypsin staining exocrine gland in rat pancreas.
Panel C : anti-Claudin 3 staining cell membrane in rat pancreas.
Panel D : anti-C Peptide staining islet cells in rat pancreas.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab318203, ab317319 and ab314215 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal^™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-C Peptide antibody [EPR25854-146] (AB314215)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded rat pancreas tissue staining Carboxypeptidase A with ab278044 at a 1/4000 dilution, ab317319 anti-Claudin-3 used at 1/2000 dilution and ab314215 anti-C Peptide used at a 1/500 dilution. Opal Polymer HRP Ms + Rb was used as a secondary antibody.

Panel A : merged staining of anti-Carboxypeptidase A (green; Opal^™520), anti-Claudin 3 (magenta; Opal^™690) and anti-C Peptide (gray; Opal^™570) on rat pancreas. Panel B : anti-Carboxypeptidase A staining acinar cells in rat pancreas.
Panel C : ant-Claudin 3 staining cell membrane in rat pancreas.
Panel D : ant-C Peptide staining islet cells in rat pancreas.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab278044, ab317319 and ab314215 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal^™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• I-ELISA

Supplier Data

Indirect ELISA - Anti-C Peptide antibody [EPR25854-146] (AB314215)

Indirect ELISA analysis of ab314215 at 500-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1 : 2500 dilution. Antigen : Rat C-peptide of insulin-1,Rat C-peptide of Insulin-2,Mouse C-peptide of Insulin-1,Rat proinsulin-1, Mouse proinsulin-1. Antigen concentration : 100 ng/ml