Anti-C Peptide antibody [EPR25854-146] - BSA and Azide free (ab314216)

Rabbit Recombinant Monoclonal Insulin antibody. Carrier free. Suitable for mIHC, IHC-P, Flow Cyt (Intra), I-ELISA, ICC/IF and reacts with Mouse, Rat, Recombinant fragment - Mouse, Recombinant fragment - Rat samples.

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Images

Multiplex immunohistochemistry - Anti-C Peptide antibody [EPR25854-146] - BSA and Azide free (AB314216), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	mIHC	IHC-P	Flow Cyt (Intra)	I-ELISA	ICC/IF
Human	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended
Mouse	Tested	Tested	Tested	Expected	Tested
Rat	Tested	Tested	Tested	Expected	Expected
Recombinant fragment - Mouse	Not recommended	Not recommended	Not recommended	Tested	Not recommended
Recombinant fragment - Rat	Not recommended	Not recommended	Not recommended	Tested	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Recombinant fragment - Mouse, Recombinant fragment - Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Recombinant fragment - Mouse, Recombinant fragment - Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Recombinant fragment - Mouse, Recombinant fragment - Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Recombinant fragment - Mouse, Recombinant fragment - Rat	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Recombinant fragment - Mouse, Recombinant fragment - Rat	Dilution info -	Notes -

Associated Products

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1 product for Alternative Version

Target data

See full target information Ins1

Function

Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.

Alternative names

Ins-1, Ins1, Insulin-1

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: EPR25854-146
Purification technique: Affinity purification Protein A
Specificity: This antibody specifically recognizes the C peptide of both INS1 and INS2, but not proinsulin.
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C

Notes

ab314216 is the carrier-free version of Anti-C Peptide antibody [EPR25854-146] ab314215.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

C-peptide also known as connecting peptide is a short polypeptide chain consisting of 31 amino acids with a molecular weight of about 3 kDa. It results from enzymatic cleavage during the conversion of proinsulin to insulin and is co-secreted equimolarly with insulin by beta cells of the pancreas. C-peptide does not undergo significant liver metabolism making its levels a meaningful indicator of endogenous insulin production. This peptide is important due to its ability to serve as a biomarker for beta cell function giving insights into the body's insulin production capacity. Commercially available assays for its measurement include C-peptide ELISA and C-peptide ELISA kit important in both clinical and research settings.

Biological function summary

C-peptide plays a role beyond being a mere byproduct of insulin synthesis. Experiments have shown it binds to cell membranes indicating it functions independently and is more than part of the insulin processing complex. Studies suggest C-peptide may have physiological effects such as improving blood flow and possessing anti-inflammatory properties. Its ability to bind likely involves specific interactions that suggest possible receptors a subject of ongoing research. Detection methods like peptide ELISA and peptide test kit further facilitate studying its biological activity and potential therapeutic implications.

Pathways

C-peptide is closely linked to the metabolic insulin signaling pathway. This pathway is integral in glucose homeostasis and involves proteins such as insulin receptor and glucose transporter. Although not directly involved in the receptor-mediated actions of insulin C-peptide's presence alongside insulin shortly after release places it within this critical pathway. Another related pathway includes peptide hormone responses where C-peptide might influence vasodilation or cellular signaling revealing potential avenues for regulating metabolic conditions.

Associated diseases and disorders

C-peptide is most notably associated with diabetes mellitus specifically type 1 and type 2. Its measurement helps to distinguish between the types of diabetes as it reflects the pancreas's ability to produce insulin. Lower levels often correlate with type 1 diabetes due to autoimmune destruction of beta cells whereas type 2 diabetes may still show normal C-peptide levels as the disease progresses. C-peptide also contributes to diabetic complications through associations with proteins involved in vascular function suggesting its possible role in conditions like diabetic nephropathy and retinopathy.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

15 product images

Multiplex immunohistochemistry - Anti-C Peptide antibody [EPR25854-146] - BSA and Azide free (ab314216)
C Peptide Multiplex immunohistochemistry staining of Mouse pancreas tissue using rabbit Anti-C Peptide antibody
This data was developed using Anti-C Peptide antibody [EPR25854-146] ab314215, the same antibody clone in a different buffer formulation.
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded ^™ tissue staining C Peptide with Anti-C Peptide antibody [EPR25854-146] ab314215 at a 1/500 dilution, ab318203 anti-Chymotrypsin used at 1/6000 dilution and Anti-Cytokeratin 19 antibody [EP1580Y] - Cytoskeleton Marker ab52625 anti-Cytokeratin 19 used at a 1/6000 dilution.
Opal Polymer HRP Ms + Rb was used as a secondary antibody.
Panel A: merged staining of anti-C Peptide (green; Opal^™520), anti-Chymotrypsin (magenta; Opal^™690) and anti-Cytokeratin 19 (grey; Opal^™570) on mouse pancreas.

Panel B: anti-C Peptide staining beta cells in mouse pancreas.

Panel C: anti-Chymotrypsin staining exocrine gland in mouse pancreas.

Panel D: anti-Cytokeratin 19 staining pancreatic duct in mouse pancreas.

Nuclear DNA was labeled with DAPI (shown in blue).
The section was incubated in three rounds of staining: in the order of Anti-C Peptide antibody [EPR25854-146] ab314215, ab318203 and Anti-Cytokeratin 19 antibody [EP1580Y] - Cytoskeleton Marker ab52625 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal^™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Multiplex immunohistochemistry - Anti-C Peptide antibody [EPR25854-146] - BSA and Azide free (ab314216)
C Peptide Multiplex immunohistochemistry staining of Rat pancreas using rabbit Anti-C Peptide antibody
This data was developed using Anti-C Peptide antibody [EPR25854-146] ab314215, the same antibody clone in a different buffer formulation.
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded ^® tissue staining C Peptide with Anti-C Peptide antibody [EPR25854-146] ab314215 at a 1/500 dilution, ab318203 anti-Chymotrypsin used at 1/6000 dilution and Anti-Cytokeratin 19 antibody [EP1580Y] - Cytoskeleton Marker ab52625 anti-Cytokeratin 19 used at a 1/6000 dilution.
Opal Polymer HRP Ms + Rb was used as a secondary antibody.
Panel A: merged staining of anti-C Peptide (green; Opal^™520), anti-Chymotrypsin (magenta; Opal^™690) and anti-Cytokeratin 19 (gray; Opal^™570) on rat pancreas.

Panel B: anti-C Peptide staining beta cells in rat pancreas.

Panel C: anti-Chymotrypsin staining exocrine gland in rat pancreas.

Panel D: anti-Cytokeratin 19 staining pancreatic duct in rat pancreas.

Nuclear DNA was labeled with DAPI (shown in blue).
The section was incubated in three rounds of staining: in the order of Anti-C Peptide antibody [EPR25854-146] ab314215, ab318203 and Anti-Cytokeratin 19 antibody [EP1580Y] - Cytoskeleton Marker ab52625 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal^™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Multiplex immunohistochemistry - Anti-C Peptide antibody [EPR25854-146] - BSA and Azide free (ab314216)
C Peptide Multiplex immunohistochemistry staining of Rat pancreas tissue using rabbit Anti-C Peptide antibody
This data was developed using Anti-C Peptide antibody [EPR25854-146] ab314215, the same antibody clone in a different buffer formulation.
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded rat pancreas tissue staining C Peptide with Anti-C Peptide antibody [EPR25854-146] ab314215 at a 1/500 dilution, Anti-Carboxypeptidase A antibody [EPR24384-69] ab278044 anti-Carboxypeptidase A used at 1/4000 dilution and Anti-Cytokeratin 19 antibody [EP1580Y] - Cytoskeleton Marker ab52625 anti-Cytokeratin 19 used at a 1/6000 dilution.
Opal Polymer HRP Ms + Rb was used as a secondary antibody.
Panel A: merged staining of anti-C Peptide (green; Opal^™520), anti-Carboxypeptidase A (magenta; Opal^™690) and anti-Cytokeratin 19 (grey; Opal^™570) on rat pancreas.

Panel B: anti-C Peptide staining beta cells in rat pancreas.

Panel C: anti-Carboxypeptidase A staining exocrine gland in rat pancreas.

Panel D: anti-Cytokeratin 19 staining pancreatic duct in rat pancreas.

Nuclear DNA was labeled with DAPI (shown in blue).
The section was incubated in three rounds of staining: in the order of Anti-C Peptide antibody [EPR25854-146] ab314215, Anti-Carboxypeptidase A antibody [EPR24384-69] ab278044 and Anti-Cytokeratin 19 antibody [EP1580Y] - Cytoskeleton Marker ab52625 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal^™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Multiplex immunohistochemistry - Anti-C Peptide antibody [EPR25854-146] - BSA and Azide free (ab314216)
C Peptide Multiplex immunohistochemistry staining of mouse pancreas tissue using rabbit Anti-C Peptide antibody
This data was developed using Anti-C Peptide antibody [EPR25854-146] ab314215, the same antibody clone in a different buffer formulation.
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse pancreas tissue staining Chymotrypsin with ab318203 at a 1/6000 dilution, Anti-Claudin 3 antibody [EPR28731-2] ab317319 anti-Claudin-3 used at 1/2000 dilution and Anti-C Peptide antibody [EPR25854-146] ab314215 anti-C Peptide used at a 1/500 dilution. Opal Polymer HRP Ms + Rb was used as a secondary antibody.
Panel A: merged staining of anti-Chymotrypsin (green; Opal^™520), anti-Claudin 3 (magenta; Opal^™690) and anti-C Peptide (gray; Opal^™570) on mouse pancreas.

Panel B: anti-Chymotrypsin staining exocrine gland in mouse pancreas.

Panel C: anti-Claudin 3 staining cell membrane in mouse pancreas.

Panel D: anti-C Peptide staining islet cells in mouse pancreas.
Nuclear DNA was labeled with DAPI (shown in blue).
The section was incubated in three rounds of staining: in the order of ab318203, Anti-Claudin 3 antibody [EPR28731-2] ab317319 and Anti-C Peptide antibody [EPR25854-146] ab314215 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal^™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Multiplex immunohistochemistry - Anti-C Peptide antibody [EPR25854-146] - BSA and Azide free (ab314216)
C Peptide Multiplex immunohistochemistry staining of rat pancreas tissue using rabbit Anti-C Peptide antibody
This data was developed using Anti-C Peptide antibody [EPR25854-146] ab314215, the same antibody clone in a different buffer formulation.
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded rat pancreas tissue staining Chymotrypsin with ab318203 at a 1/6000 dilution, Anti-Claudin 3 antibody [EPR28731-2] ab317319 anti-Claudin-3 used at 1/2000 dilution and Anti-C Peptide antibody [EPR25854-146] ab314215 anti-C Peptide used at a 1/500 dilution. Opal Polymer HRP Ms + Rb was used as a secondary antibody.
Panel A: merged staining of anti-Chymotrypsin (green; Opal^™520), anti-Claudin 3 (magenta; Opal^™690) and anti-C Peptide (gray; Opal^™570) on rat pancreas.

Panel B: anti-Chymotrypsin staining exocrine gland in rat pancreas.

Panel C: anti-Claudin 3 staining cell membrane in rat pancreas.

Panel D: anti-C Peptide staining islet cells in rat pancreas.
Nuclear DNA was labeled with DAPI (shown in blue).
The section was incubated in three rounds of staining: in the order of ab318203, Anti-Claudin 3 antibody [EPR28731-2] ab317319 and Anti-C Peptide antibody [EPR25854-146] ab314215 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal^™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Western blot - Anti-C Peptide antibody [EPR25854-146] - BSA and Azide free (ab314216)
C Peptide Western blot staining using rabbit Anti-C Peptide antibody
This data was developed using Anti-C Peptide antibody [EPR25854-146] ab314215, the same antibody clone in a different buffer formulation.
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded rat pancreas tissue staining Chymotrypsin with ab318203 at a 1/6000 dilution, Anti-Claudin 3 antibody [EPR28731-2] ab317319 anti-Claudin-3 used at 1/2000 dilution and Anti-C Peptide antibody [EPR25854-146] ab314215 anti-C Peptide used at a 1/500 dilution. Opal Polymer HRP Ms + Rb was used as a secondary antibody.
Panel A: merged staining of anti-Chymotrypsin (green; Opal^™520), anti-Claudin 3 (magenta; Opal^™690) and anti-C Peptide (gray; Opal^™570) on rat pancreas.

Panel B: anti-Chymotrypsin staining exocrine gland in rat pancreas.

Panel C: anti-Claudin 3 staining cell membrane in rat pancreas.

Panel D: anti-C Peptide staining islet cells in rat pancreas.
Nuclear DNA was labeled with DAPI (shown in blue).
The section was incubated in three rounds of staining: in the order of ab318203, Anti-Claudin 3 antibody [EPR28731-2] ab317319 and Anti-C Peptide antibody [EPR25854-146] ab314215 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal^™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
All lanes: Western blot - Anti-RAB35 antibody [EPR24487-21] (Anti-RAB35 antibody [EPR24487-21] ab300116) at 1/1000 dilution
Lane 1: C6 (rat glial tumor glial cell) whole cell lysate at 20 µg
Lane 2: RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg
Lane 3: PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate at 20 µg
Lane 4: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/10000 dilution
Predicted band size: 23 kDa
Observed band size: 25 kDa
Exposure time: 3min
Multiplex immunohistochemistry - Anti-C Peptide antibody [EPR25854-146] - BSA and Azide free (ab314216)
C Peptide Multiplex immunohistochemistry staining of Rat pancreas tissue using rabbit Anti-C Peptide antibody
This data was developed using Anti-C Peptide antibody [EPR25854-146] ab314215, the same antibody clone in a different buffer formulation.
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded rat pancreas tissue staining Carboxypeptidase A with Anti-Carboxypeptidase A antibody [EPR24384-69] ab278044 at a 1/4000 dilution, Anti-Claudin 3 antibody [EPR28731-2] ab317319 anti-Claudin-3 used at 1/2000 dilution and Anti-C Peptide antibody [EPR25854-146] ab314215 anti-C Peptide used at a 1/500 dilution. Opal Polymer HRP Ms + Rb was used as a secondary antibody.
Panel A: merged staining of anti-Carboxypeptidase A (green; Opal^™520), anti-Claudin 3 (magenta; Opal^™690) and anti-C Peptide (gray; Opal^™570) on rat pancreas.
Panel B: anti-Carboxypeptidase A staining acinar cells in rat pancreas.

Panel C: ant-Claudin 3 staining cell membrane in rat pancreas.

Panel D: ant-C Peptide staining islet cells in rat pancreas.
Nuclear DNA was labeled with DAPI (shown in blue).
The section was incubated in three rounds of staining: in the order of Anti-Carboxypeptidase A antibody [EPR24384-69] ab278044, Anti-Claudin 3 antibody [EPR28731-2] ab317319 and Anti-C Peptide antibody [EPR25854-146] ab314215 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal^™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Indirect ELISA - Anti-C Peptide antibody [EPR25854-146] - BSA and Azide free (ab314216)
This data was developed using Anti-C Peptide antibody [EPR25854-146] ab314215, the same antibody clone in a different buffer formulation.
Indirect ELISA analysis of Anti-C Peptide antibody [EPR25854-146] ab314215 at 500-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1:2500 dilution.
Antigen: Rat C-peptide of insulin-1,Rat C-peptide of Insulin-2,Mouse C-peptide of Insulin-1,Rat proinsulin-1, Mouse proinsulin-1.
Antigen concentration: 100 ng/ml
Flow Cytometry (Intracellular) - Anti-C Peptide antibody [EPR25854-146] - BSA and Azide free (ab314216)
This data was developed using Anti-C Peptide antibody [EPR25854-146] ab314215, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Isotype (Left) / AR42J (rat pancreatic tumor epithelial cell) treated with 100pM HGF and 2nM Activin A for 7days (Middle) / Untreated AR42Jcells labelling C-Peptide with Anti-C Peptide antibody [EPR25854-146] ab314215 at 1/50 dilution (1 ug)/Right (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody. PMID: 8756573. According to the literature, about 10% induced cells can express the insulin.
Flow Cytometry (Intracellular) - Anti-C Peptide antibody [EPR25854-146] - BSA and Azide free (ab314216)
This data was developed using Anti-C Peptide antibody [EPR25854-146] ab314215, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (mouse embryonic fibroblast, Left) / Beta-TC-6 (mouse pancreas insulinoma beta cell, Right) cells labelling C-Peptide with Anti-C Peptide antibody [EPR25854-146] ab314215 at 1/500 dilution (0.1 ug)/Red (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody. Negative control: NIH/3T3 (PMID: 23662125).
Immunocytochemistry/ Immunofluorescence - Anti-C Peptide antibody [EPR25854-146] - BSA and Azide free (ab314216)
This data was developed using Anti-C Peptide antibody [EPR25854-146] ab314215, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Beta-TC-6 (mouse pancreas insulinoma beta cell) cells labelling C-Peptide with Anti-C Peptide antibody [EPR25854-146] ab314215 at 1/500 (1.086 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing cytoplasmic staining in Beta-TC-6 cell line. Negative control: NIH/3T3 (PMID: 23662125). Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml)) dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Peptide antibody [EPR25854-146] - BSA and Azide free (ab314216)
This data was developed using Anti-C Peptide antibody [EPR25854-146] ab314215, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat cardiac muscle tissue labeling C-Peptide with Anti-C Peptide antibody [EPR25854-146] ab314215 at 1/2000 (0.272 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: no staining on rat cardiac muscle. The section was incubated with Anti-C Peptide antibody [EPR25854-146] ab314215 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Peptide antibody [EPR25854-146] - BSA and Azide free (ab314216)
This data was developed using Anti-C Peptide antibody [EPR25854-146] ab314215, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue labeling C-Peptide with Anti-C Peptide antibody [EPR25854-146] ab314215 at 1/500 (1.086 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: no staining on mouse cardiac muscle. The section was incubated with Anti-C Peptide antibody [EPR25854-146] ab314215 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Peptide antibody [EPR25854-146] - BSA and Azide free (ab314216)
This data was developed using Anti-C Peptide antibody [EPR25854-146] ab314215, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat pancreas tissue labeling C-Peptide with Anti-C Peptide antibody [EPR25854-146] ab314215 at 1/2000 (0.272 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on islet of rat pancreas. The section was incubated with Anti-C Peptide antibody [EPR25854-146] ab314215 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Peptide antibody [EPR25854-146] - BSA and Azide free (ab314216)
This data was developed using Anti-C Peptide antibody [EPR25854-146] ab314215, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue labeling C-Peptide with Anti-C Peptide antibody [EPR25854-146] ab314215 at 1/500 (1.086 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on islet of mouse pancreas. The section was incubated with Anti-C Peptide antibody [EPR25854-146] ab314215 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

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Anti-C Peptide antibody [EPR25854-146] - BSA and Azide free

Images

Key facts

Immunogen

Reactivity data

mIHC

TestedTested

Not recommendedNot recommended

IHC-P

TestedTested

Not recommendedNot recommended

Flow Cyt (Intra)

TestedTested

Not recommendedNot recommended

I-ELISA

TestedTested

ExpectedExpected

Not recommendedNot recommended

ICC/IF

TestedTested

ExpectedExpected

Not recommendedNot recommended

Associated Products

1 product for Alternative Version

Target data

Function

Alternative names

Recommended products

Key facts

Storage

Notes

Supplementary info

Product promise

Tested

Expected

Predicted

Not recommended

15 product images

Multiplex immunohistochemistry - Anti-C Peptide antibody [EPR25854-146] - BSA and Azide free (ab314216)

Multiplex immunohistochemistry - Anti-C Peptide antibody [EPR25854-146] - BSA and Azide free (ab314216)

Multiplex immunohistochemistry - Anti-C Peptide antibody [EPR25854-146] - BSA and Azide free (ab314216)

Multiplex immunohistochemistry - Anti-C Peptide antibody [EPR25854-146] - BSA and Azide free (ab314216)

Multiplex immunohistochemistry - Anti-C Peptide antibody [EPR25854-146] - BSA and Azide free (ab314216)

Western blot - Anti-C Peptide antibody [EPR25854-146] - BSA and Azide free (ab314216)

Multiplex immunohistochemistry - Anti-C Peptide antibody [EPR25854-146] - BSA and Azide free (ab314216)

Indirect ELISA - Anti-C Peptide antibody [EPR25854-146] - BSA and Azide free (ab314216)

Flow Cytometry (Intracellular) - Anti-C Peptide antibody [EPR25854-146] - BSA and Azide free (ab314216)

Flow Cytometry (Intracellular) - Anti-C Peptide antibody [EPR25854-146] - BSA and Azide free (ab314216)

Immunocytochemistry/ Immunofluorescence - Anti-C Peptide antibody [EPR25854-146] - BSA and Azide free (ab314216)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Peptide antibody [EPR25854-146] - BSA and Azide free (ab314216)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Peptide antibody [EPR25854-146] - BSA and Azide free (ab314216)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Peptide antibody [EPR25854-146] - BSA and Azide free (ab314216)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Peptide antibody [EPR25854-146] - BSA and Azide free (ab314216)

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Product protocols

Tested
Tested

Not recommended
Not recommended

Tested
Tested

Not recommended
Not recommended

Tested
Tested

Not recommended
Not recommended

Tested
Tested

Expected
Expected

Not recommended
Not recommended

Tested
Tested

Expected
Expected

Not recommended
Not recommended