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AB8278

Anti-C Reactive Protein antibody [C6]

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(6 Publications)

Mouse Monoclonal C Reactive Protein antibody. Suitable for sELISA and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human CRP.

View Alternative Names

PTX1, CRP, C-reactive protein

2 Images
Sandwich ELISA - Anti-C Reactive Protein antibody [C6] (AB8278)
  • sELISA

Unknown

Sandwich ELISA - Anti-C Reactive Protein antibody [C6] (AB8278)

Calibration curve using a biotinylated version of ab136176 [C2] and ab8278 [C6] (labelled with stable Eu3+ chelate) in a sandwich ELISA. The mixture of antibodies and antigen (10 µl sample) was incubated for 10 minutes at RT in streptavidin-coated plates.

The antibody used as its pair (ab8277) is no longer available, but the unconjugated version is as ab136176.

Sandwich ELISA - Anti-C Reactive Protein antibody [C6] (AB8278)
  • sELISA

Unknown

Sandwich ELISA - Anti-C Reactive Protein antibody [C6] (AB8278)

Sensitivity to EDTA. Serum samples with (dark blue) and w/o (light blue) Ca2+ ions were tested by two two-site combinations of anti-CRP MAbs. [C4] is sensitive to the conformational changes of CRP molecule and thus the assay gives no signal in the presence of EDTA. Sandwich immunoassay utilizing a biotinylated version of ab136176 [C2] and ab8278 [C6] recognizes the antigen with the same efficiency in the presence and in the absence of EDTA.

The antibody used as its pair (ab8277) is no longer available, but the unconjugated version is as ab136176.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

C6

Isotype

IgG2a

Light chain type

unknown

Carrier free

No

Reacts with

Human

Applications

sELISA

applications

Immunogen

Native Full Length Protein corresponding to Human CRP.

P02741

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p>Recommended pairs for sandwich immunoassay (capture - detection): C2 - C6.</p>" } } }
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Product details

Specific antibody titer in ELISA is > 10 to the power of -6
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

C-reactive protein (CRP) also known as CRP protein is an acute-phase protein with a molecular mass of approximately 25 kDa. It is primarily synthesized in the liver in response to cytokines. This protein appears in the blood where it binds to phosphocholine expressed on the surface of dead or dying cells. CRP is often used as a biomarker for inflammation in the body and its levels in human and canine (anti CRP used) blood samples are measured using assays like the CRP ELISA test or CRP ELISA kit.
Biological function summary

CRP plays a significant role in the innate immune system by recognizing foreign pathogens and damaged cells. It does not form part of a larger complex but itself can bind to specific ligands initiating the complement cascade. This activation enhances phagocytosis by macrophages contributing to the clearance of necrotic and apoptotic cells. The CRP protein therefore acts as a fast responder in the presence of inflammation making it a useful indicator for monitoring inflammatory diseases.

Pathways

CRP operates predominantly within the acute-phase response pathway. Its production is upregulated by cytokines such as IL-6 which connects it to inflammation-signaling pathways. CRP also interacts with the complement system bridging innate and adaptive immunity. Besides CRP could indirectly influence pathways involving other inflammatory proteins like serum amyloid A and haptoglobin creating a network of responses important for managing inflammation.

Elevated CRP levels are commonly associated with cardiovascular diseases and rheumatoid arthritis. In cardiovascular diseases CRP acts as a marker for atherosclerosis where the protein levels correlate with disease severity. It connects with other proteins involved in inflammatory processes such as fibrinogen to contribute to disease pathology. In rheumatoid arthritis CRP provides insights into disease activity helping in the assessment of treatment responses.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Displays several functions associated with host defense : it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.
See full target information CRP
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Biochip journal 16:480-489 PubMed36320437

2022

Pressed Lateral Flow Assay Strips for Flow Delay-Induced Signal Enhancement in Lateral Flow Assay Strips.

Applications

Unspecified application

Species

Unspecified reactive species

Se Been Park,Joong Ho Shin

Talanta 240:123157 PubMed34968809

2021

Mitigating hook effect in one-step quantitative sandwich lateral flow assay by timed conjugate release.

Applications

Unspecified application

Species

Unspecified reactive species

Guozhen He,Tao Dong,Zhaochu Yang,Zhuangde Jiang

Biosensors & bioelectronics 132:162-170 PubMed30875628

2019

Nanoplasmonic swarm biosensing using single nanoparticle colorimetry.

Applications

Unspecified application

Species

Unspecified reactive species

Mengxing Ouyang,Dino Di Carlo

Talanta 199:164-169 PubMed30952241

2019

Metal-enhanced chemiluminescence detection of C-reaction protein based on silver nanoparticle hybrid probes.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Zong,Duoduo Zhang,Fan Jiang,Hua Yang,Shijia Liu,Ping Li

RSC advances 8:14510-14514 PubMed35540764

2018

An optical pickup enzyme-linked immunosorbent assay (ELISA) with a microfluidic disk.

Applications

Unspecified application

Species

Unspecified reactive species

H Yoshikawa,M Yoshinaga,E Tamiya

Antioxidants & redox signaling 26:193-206 PubMed27488221

2016

Administration of Pure Ergothioneine to Healthy Human Subjects: Uptake, Metabolism, and Effects on Biomarkers of Oxidative Damage and Inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Irwin K Cheah,Richard M Y Tang,Terry S Z Yew,Keith H C Lim,Barry Halliwell
View all publications
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