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Rabbit Recombinant Monoclonal C Reactive Protein antibody. Suitable for IP, WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 2 publications.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Reactive Protein antibody [EPR23975-119] (AB259862), expandable thumbnail
  • Immunoprecipitation - Anti-C Reactive Protein antibody [EPR23975-119] (AB259862), expandable thumbnail
  • Western blot - Anti-C Reactive Protein antibody [EPR23975-119] (AB259862), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Reactive Protein antibody [EPR23975-119] (AB259862), expandable thumbnail
  • Western blot - Anti-C Reactive Protein antibody [EPR23975-119] (AB259862), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPWBIHC-FrFlow Cyt (Intra)IHC-P
Human
Not recommended
Expected
Tested
Not recommended
Not recommended
Not recommended
Mouse
Not recommended
Tested
Tested
Not recommended
Not recommended
Tested
Rat
Not recommended
Expected
Tested
Not recommended
Not recommended
Tested

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

-

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/30
Notes

-

Expected
Expected

Species
Rat, Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Rat
Dilution info
1/2000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Mouse
Dilution info
1/2000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target data

Function

Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.

Alternative names

PTX1, CRP, C-reactive protein

Recommended products

Rabbit Recombinant Monoclonal C Reactive Protein antibody. Suitable for IP, WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 2 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR23975-119
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

C-reactive protein (CRP) also known as CRP protein is an acute-phase protein with a molecular mass of approximately 25 kDa. It is primarily synthesized in the liver in response to cytokines. This protein appears in the blood where it binds to phosphocholine expressed on the surface of dead or dying cells. CRP is often used as a biomarker for inflammation in the body and its levels in human and canine (anti CRP used) blood samples are measured using assays like the CRP ELISA test or CRP ELISA kit.

Biological function summary

CRP plays a significant role in the innate immune system by recognizing foreign pathogens and damaged cells. It does not form part of a larger complex but itself can bind to specific ligands initiating the complement cascade. This activation enhances phagocytosis by macrophages contributing to the clearance of necrotic and apoptotic cells. The CRP protein therefore acts as a fast responder in the presence of inflammation making it a useful indicator for monitoring inflammatory diseases.

Pathways

CRP operates predominantly within the acute-phase response pathway. Its production is upregulated by cytokines such as IL-6 which connects it to inflammation-signaling pathways. CRP also interacts with the complement system bridging innate and adaptive immunity. Besides CRP could indirectly influence pathways involving other inflammatory proteins like serum amyloid A and haptoglobin creating a network of responses important for managing inflammation.

Associated diseases and disorders

Elevated CRP levels are commonly associated with cardiovascular diseases and rheumatoid arthritis. In cardiovascular diseases CRP acts as a marker for atherosclerosis where the protein levels correlate with disease severity. It connects with other proteins involved in inflammatory processes such as fibrinogen to contribute to disease pathology. In rheumatoid arthritis CRP provides insights into disease activity helping in the assessment of treatment responses.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Reactive Protein antibody [EPR23975-119] (ab259862), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Reactive Protein antibody [EPR23975-119] (ab259862)

    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling C Reactive Protein with ab259862 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Cytoplasmic staining in mouse liver. The section was incubated with ab259862 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunoprecipitation - Anti-C Reactive Protein antibody [EPR23975-119] (ab259862), expandable thumbnail

    Immunoprecipitation - Anti-C Reactive Protein antibody [EPR23975-119] (ab259862)

    C Reactive Protein was immunoprecipitated from 0.35 mg Mouse serum with ab259862 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab259862 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: Mouse serum 10 ug

    Lane 2: ab259862 IP in Mouse serum

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab259862 in mouse serum

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 32 seconds

    All lanes: Immunoprecipitation - Anti-C Reactive Protein antibody [EPR23975-119] (ab259862)

    Predicted band size: 25 kDa

    Observed band size: 27 kDa

  • Western blot - Anti-C Reactive Protein antibody [EPR23975-119] (ab259862), expandable thumbnail

    Western blot - Anti-C Reactive Protein antibody [EPR23975-119] (ab259862)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Exposure time: 3.25 seconds

    All lanes: Western blot - Anti-C Reactive Protein antibody [EPR23975-119] (ab259862) at 1/1000 dilution

    Lane 1: Human liver tissue lysate at 20 µg

    Lane 2: Human kidney tissue lysate at 20 µg

    Lane 3: Human plasma at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 25 kDa

    Observed band size: 27 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Reactive Protein antibody [EPR23975-119] (ab259862), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Reactive Protein antibody [EPR23975-119] (ab259862)

    Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling C Reactive Protein with ab259862 at 1/10000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Cytoplasmic staining in rat liver. The section was incubated with ab259862 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Western blot - Anti-C Reactive Protein antibody [EPR23975-119] (ab259862), expandable thumbnail

    Western blot - Anti-C Reactive Protein antibody [EPR23975-119] (ab259862)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Exposure time: 37 seconds

    All lanes: Western blot - Anti-C Reactive Protein antibody [EPR23975-119] (ab259862) at 1/2000 dilution

    Lane 1: Mouse plasma at 10 µg

    Lane 2: Mouse serum at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/5000 dilution

    Predicted band size: 25 kDa

    Observed band size: 27 kDa

  • Western blot - Anti-C Reactive Protein antibody [EPR23975-119] (ab259862), expandable thumbnail

    Western blot - Anti-C Reactive Protein antibody [EPR23975-119] (ab259862)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Exposure time: 1 second

    All lanes: Western blot - Anti-C Reactive Protein antibody [EPR23975-119] (ab259862) at 1/1000 dilution

    Lane 1: Rat liver tissue lysate at 20 µg

    Lane 2: Rat kidney tissue lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 25 kDa

    Observed band size: 27 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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