Anti-C Reactive Protein antibody [EPR23975-119] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Reactive Protein antibody [EPR23975-119] - BSA and Azide free (AB280362)

This data was developed using ab259862, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling C Reactive Protein with ab259862 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining in mouse liver. The section was incubated with ab259862 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Reactive Protein antibody [EPR23975-119] - BSA and Azide free (AB280362)

This data was developed using ab259862, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling C Reactive Protein with ab259862 at 1/10000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining in rat liver. The section was incubated with ab259862 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IP

Supplier Data

Immunoprecipitation - Anti-C Reactive Protein antibody [EPR23975-119] - BSA and Azide free (AB280362)

This data was developed using ab259862, the same antibody clone in a different buffer formulation.

C Reactive Protein was immunoprecipitated from 0.35 mg Mouse serum with ab259862 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab259862 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Mouse serum 10 ug

Lane 2 : ab259862 IP in Mouse serum

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab259862 in mouse serum

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 32 seconds

All lanes:

Immunoprecipitation - Anti-C Reactive Protein antibody [EPR23975-119] (<a href='/en-us/products/primary-antibodies/c-reactive-protein-antibody-epr23975-119-ab259862'>ab259862</a>)

Predicted band size: 25 kDa

Observed band size: 27 kDa

false

• WB

Lab

Western blot - Anti-C Reactive Protein antibody [EPR23975-119] - BSA and Azide free (AB280362)

This data was developed using ab259862, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration :

Exposure time : 3.25 seconds

All lanes:

Western blot - Anti-C Reactive Protein antibody [EPR23975-119] (<a href='/en-us/products/primary-antibodies/c-reactive-protein-antibody-epr23975-119-ab259862'>ab259862</a>) at 1/1000 dilution

Lane 1:

Human liver tissue lysate at 20 µg

Lane 2:

Human kidney tissue lysate at 20 µg

Lane 3:

Human plasma at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 25 kDa

Observed band size: 27 kDa

false

• WB

Lab

Western blot - Anti-C Reactive Protein antibody [EPR23975-119] - BSA and Azide free (AB280362)

This data was developed using ab259862, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Exposure time : 1 second

All lanes:

Western blot - Anti-C Reactive Protein antibody [EPR23975-119] (<a href='/en-us/products/primary-antibodies/c-reactive-protein-antibody-epr23975-119-ab259862'>ab259862</a>) at 1/1000 dilution

Lane 1:

Rat liver tissue lysate at 20 µg

Lane 2:

Rat kidney tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 25 kDa

Observed band size: 27 kDa

false

• WB

Lab

Western blot - Anti-C Reactive Protein antibody [EPR23975-119] - BSA and Azide free (AB280362)

This data was developed using ab259862, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Exposure time : 37 seconds

All lanes:

Western blot - Anti-C Reactive Protein antibody [EPR23975-119] (<a href='/en-us/products/primary-antibodies/c-reactive-protein-antibody-epr23975-119-ab259862'>ab259862</a>) at 1/2000 dilution

Lane 1:

Mouse plasma at 10 µg

Lane 2:

Mouse serum at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/5000 dilution

Predicted band size: 25 kDa

Observed band size: 27 kDa

false