Anti-C Reactive Protein antibody [Y284] (ab32412) is a rabbit monoclonal antibody that is used to detect C Reactive Protein in Western Blot, IP, IHC-P. Suitable for Human samples.
- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency
-Over 30 publications
-Over 30 publications
Images
![Western blot - Anti-C Reactive Protein antibody [Y284] (AB32412), expandable thumbnail](https://content.abcam.com/products/images/c-reactive-protein-antibody-y284-ab32412--western-blot-img146750.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-C Reactive Protein antibody [Y284] (AB32412)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Reactive Protein antibody [Y284] (AB32412), expandable thumbnail](https://content.abcam.com/products/images/c-reactive-protein-antibody-y284-ab32412--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img91924.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Reactive Protein antibody [Y284] (AB32412)")
![Immunoprecipitation - Anti-C Reactive Protein antibody [Y284] (AB32412), expandable thumbnail](https://content.abcam.com/products/images/c-reactive-protein-antibody-y284-ab32412--immunoprecipitation-img59496.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-C Reactive Protein antibody [Y284] (AB32412)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Reactive Protein antibody [Y284] (AB32412), expandable thumbnail](https://content.abcam.com/products/images/c-reactive-protein-antibody-y284-ab32412--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img14280.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Reactive Protein antibody [Y284] (AB32412)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Reactive Protein antibody [Y284] (AB32412), expandable thumbnail](https://content.abcam.com/products/images/c-reactive-protein-antibody-y284-ab32412--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img135747.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Reactive Protein antibody [Y284] (AB32412)")
Publications
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- iScience 26:1062472023Deciduous tooth biomarkers reveal atypical fetal inflammatory regulation in autism spectrum disorder.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDani Dumitriu,Elena Baldwin,Roozie J J Coenen,Luke A Hammond,Darcy S Peterka,Lynne Heilbrun,Richard E Frye,Raymond Palmer,Hjalmar Nobel Norrman,Anna Fridell,Karl Lundin Remnelius,Johan Isaksson,Christine Austin,Paul Curtin,Sven Bölte,Manish AroraPubMed 36926653
- EBioMedicine 77:1039032022Alzheimer's disease protease-containing plasma extracellular vesicles transfer to the hippocampus via the choroid plexus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJung-Hyun Lee,Christian Ostalecki,Timo Oberstein,Stefan Schierer,Elisabeth Zinser,Martin Eberhardt,Katja Blume,Bianca Plosnita,Lena Stich,Heiko Bruns,Roland Coras,Julio Vera-Gonzales,Manuel Maler,Andreas S BaurPubMed 35220044
- Communications medicine 2:112022Genomic analysis of focal nodular hyperplasia with associated hepatocellular carcinoma unveils its malignant potential: a case report.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCaner Ercan,Mairene Coto-Llerena,John Gallon,Lana Fourie,Mattia Marinucci,Gabriel F Hess,Jürg Vosbeck,Stephanie Taha-Mehlitz,Tuyana Boldanova,Marie-Anne Meier,Alexandar Tzankov,Matthias S Matter,Martin H K Hoffmann,Luca Di Tommaso,Markus von Flüe,Charlotte K Y Ng,Markus H Heim,Savas D Soysal,Luigi M Terracciano,Otto Kollmar,Salvatore PiscuoglioPubMed 35603298
- PloS one 16:e02453612021CRP immunodeposition and proteomic analysis in abdominal aortic aneurysm.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEun Na Kim,Jiyoung Yu,Joon Seo Lim,Hwangkyo Jeong,Chong Jai Kim,Jae-Sung Choi,So Ra Kim,Hee-Sung Ahn,Kyunggon Kim,Se Jin OhPubMed 34428207
- Archives of pathology & laboratory medicine 146:220-2262021HNF-1β is a More Sensitive and Specific Marker Than C-Reactive Protein for Identifying Biliary Differentiation in Primary Hepatic Carcinomas.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPallavi A Patil,Tamar Taddei,Dhanpat Jain,Xuchen ZhangPubMed 34086854
- Heliyon 7:e071342021Pulmonary stromal expansion and intra-alveolar coagulation are primary causes of COVID-19 death.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLaszlo Szekely,Bela Bozoky,Matyas Bendek,Masih Ostad,Pablo Lavignasse,Lars Haag,Jieyu Wu,Xu Jing,Soham Gupta,Elisa Saccon,Anders Sönnerborg,Yihai Cao,Mikael Björnstedt,Attila SzakosPubMed 34056141
- Redox biology 41:1019252021Crosslinking of human plasma C-reactive protein to human serum albumin via disulfide bond oxidation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShuwen Jiang,Per Hägglund,Luke Carroll,Lars M Rasmussen,Michael J DaviesPubMed 33714740
- Redox biology 41:1018742021Formation of protein cross-links by singlet oxygen-mediated disulfide oxidation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShuwen Jiang,Luke Carroll,Michele Mariotti,Per Hägglund,Michael J DaviesPubMed 33601275
- Virchows Archiv : an international journal of path :2020Ki-67 "hot spot" digital analysis is useful in the distinction of hepatic adenomas and well-differentiated hepatocellular carcinomas.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAndrea Jones et. al.PubMed 32583014
- PloS one 14:e02166102019High serum CRP influences myocardial miRNA profiles in ischemia-reperfusion injury of rat heart.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEun Na Kim et. al.PubMed 31063484
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | ICC/IF | IHC-P | |
|---|---|---|---|---|
| Human | Tested | Tested | Not recommended | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/30 - 1/50 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/2000 | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/250 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
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1 product for Alternative Version
Target data
Function
Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.
Alternative names
PTX1, CRP, C-reactive protein
Recommended products
Anti-C Reactive Protein antibody [Y284] (ab32412) is a rabbit monoclonal antibody that is used to detect C Reactive Protein in Western Blot, IP, IHC-P. Suitable for Human samples.
- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency
-Over 30 publications
-Over 30 publications
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- Y284
- Purification technique
- Affinity purification Protein A
- Specificity
This antibody recognises C Reactive Protein. This antibody is predicted to detect the splice isoform 2 as well as isoform 1.
- Epitope
- ab32412 reacts with an epitope located in the C terminal region of C Reactive Protein.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-C Reactive Protein antibody [Y284] (ab32412) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P) in Human samples.
What is the molecular weight of C Reactive Protein?
Anti-C Reactive Protein [Y284] (ab32412) specifically detects a band for C Reactive Protein (UniProt: P02741) at a molecular weight of 25kDa.
Trusted by the scientific community
Anti-C Reactive Protein [Y284] (ab32412) was first used in a scientific publication in 2006 and has been cited over 30 times in peer-reviewed journals.
Reviewed by scientists
Anti-C Reactive Protein [Y284] (ab32412) has over 5 independent reviews from customers.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.
Other related products
We have a range of other formats of antibody clone [Y284] also available for your convenience:
ab32412, Carrier free - Anti-C Reactive Protein antibody [Y284] - Low endotoxin, Azide free ab157242, Carrier free - Anti-C Reactive Protein antibody [Y284] - BSA and Azide free ab271830, HRP - HRP Anti-C Reactive Protein antibody [Y284] ab307473, Alexa Fluor® 647 - Alexa Fluor® 647 Anti-C Reactive Protein antibody [Y284] ab307519, Alexa Fluor® 594 - Alexa Fluor® 594 Anti-C Reactive Protein antibody [Y284] ab311801, Alexa Fluor® 568 - Alexa Fluor® 568 Anti-C Reactive Protein antibody [Y284] ab313084, Alexa Fluor® 555 - Alexa Fluor® 555 Anti-C Reactive Protein antibody [Y284] ab313278, Alexa Fluor® 488 - Alexa Fluor® 488 Anti-C Reactive Protein antibody [Y284] ab314471, Alexa Fluor® 750 - Alexa Fluor® 750 Anti-C Reactive Protein antibody [Y284] ab321600
Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
C-reactive protein (CRP) also known as CRP protein is an acute-phase protein with a molecular mass of approximately 25 kDa. It is primarily synthesized in the liver in response to cytokines. This protein appears in the blood where it binds to phosphocholine expressed on the surface of dead or dying cells. CRP is often used as a biomarker for inflammation in the body and its levels in human and canine (anti CRP used) blood samples are measured using assays like the CRP ELISA test or CRP ELISA kit.
- Biological function summary
CRP plays a significant role in the innate immune system by recognizing foreign pathogens and damaged cells. It does not form part of a larger complex but itself can bind to specific ligands initiating the complement cascade. This activation enhances phagocytosis by macrophages contributing to the clearance of necrotic and apoptotic cells. The CRP protein therefore acts as a fast responder in the presence of inflammation making it a useful indicator for monitoring inflammatory diseases.
- Pathways
CRP operates predominantly within the acute-phase response pathway. Its production is upregulated by cytokines such as IL-6 which connects it to inflammation-signaling pathways. CRP also interacts with the complement system bridging innate and adaptive immunity. Besides CRP could indirectly influence pathways involving other inflammatory proteins like serum amyloid A and haptoglobin creating a network of responses important for managing inflammation.
- Associated diseases and disorders
Elevated CRP levels are commonly associated with cardiovascular diseases and rheumatoid arthritis. In cardiovascular diseases CRP acts as a marker for atherosclerosis where the protein levels correlate with disease severity. It connects with other proteins involved in inflammatory processes such as fibrinogen to contribute to disease pathology. In rheumatoid arthritis CRP provides insights into disease activity helping in the assessment of treatment responses.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Western blot - Anti-C Reactive Protein antibody [Y284] (ab32412), expandable thumbnail](https://content.abcam.com/products/images/c-reactive-protein-antibody-y284-ab32412--western-blot-img146750.jpg "Western blot - Anti-C Reactive Protein antibody [Y284] (ab32412)")
Western blot - Anti-C Reactive Protein antibody [Y284] (ab32412)
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-C Reactive Protein antibody [Y284] (ab32412) at 1/2000 dilution
Lane 1: Human serum at 20 µg
Lane 2: Human plasma at 20 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/50000 dilution
Predicted band size: 25 kDa
Observed band size: 27 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Reactive Protein antibody [Y284] (ab32412), expandable thumbnail](https://content.abcam.com/products/images/c-reactive-protein-antibody-y284-ab32412--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img91924.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Reactive Protein antibody [Y284] (ab32412)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Reactive Protein antibody [Y284] (ab32412)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle tissue labelling C Reactive Protein with purified ab32412 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
![Immunoprecipitation - Anti-C Reactive Protein antibody [Y284] (ab32412), expandable thumbnail](https://content.abcam.com/products/images/c-reactive-protein-antibody-y284-ab32412--immunoprecipitation-img59496.jpg "Immunoprecipitation - Anti-C Reactive Protein antibody [Y284] (ab32412)")
Immunoprecipitation - Anti-C Reactive Protein antibody [Y284] (ab32412)
ab32412 (purified) at 1/30 immunoprecipitating C Reactive Protein in HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate.
Lane 1 (input): HepG2 whole cell lysate (10μg)
Lane 2 (+): ab32412 + HepG2 whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab32412 in HepG2 whole cell lysate.
For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
All lanes: Immunoprecipitation - Anti-C Reactive Protein antibody [Y284] (ab32412)
Predicted band size: 25 kDa
Observed band size: 27 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Reactive Protein antibody [Y284] (ab32412), expandable thumbnail](https://content.abcam.com/products/images/c-reactive-protein-antibody-y284-ab32412--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img14280.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Reactive Protein antibody [Y284] (ab32412)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Reactive Protein antibody [Y284] (ab32412)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling C Reactive Protein with purified ab32412 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Reactive Protein antibody [Y284] (ab32412), expandable thumbnail](https://content.abcam.com/products/images/c-reactive-protein-antibody-y284-ab32412--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img135747.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Reactive Protein antibody [Y284] (ab32412)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Reactive Protein antibody [Y284] (ab32412)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded) analysis of human normal liver tissue labelling C Reactive Protein using unpurified ab32412 at a dilution of 1/100.
![Western blot - Anti-C Reactive Protein antibody [Y284] (ab32412), expandable thumbnail](https://content.abcam.com/products/images/c-reactive-protein-antibody-y284-ab32412--western-blot-img156981.gif "Western blot - Anti-C Reactive Protein antibody [Y284] (ab32412)")
Western blot - Anti-C Reactive Protein antibody [Y284] (ab32412)
All lanes: Western blot - Anti-C Reactive Protein antibody [Y284] (ab32412) at 1/1000 dilution
All lanes: Human serum
Predicted band size: 25 kDa
Observed band size: 27 kDa
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