Rabbit Recombinant Monoclonal C10 antibody. Carrier free. Suitable for IP, IHC-Fr, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C10 antibody [EPR23475-105] - BSA and Azide free (AB275039), expandable thumbnail](https://content.abcam.com/products/images/c10-antibody-epr23475-105-bsa-and-azide-free-ab275039--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img112432.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C10 antibody [EPR23475-105] - BSA and Azide free (AB275039)")
![Immunohistochemistry (Frozen sections) - Anti-C10 antibody [EPR23475-105] - BSA and Azide free (AB275039), expandable thumbnail](https://content.abcam.com/products/images/c10-antibody-epr23475-105-bsa-and-azide-free-ab275039--immunohistochemistry-frozen-sections-img60806.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-C10 antibody [EPR23475-105] - BSA and Azide free (AB275039)")
![Immunoprecipitation - Anti-C10 antibody [EPR23475-105] - BSA and Azide free (AB275039), expandable thumbnail](https://content.abcam.com/products/images/c10-antibody-epr23475-105-bsa-and-azide-free-ab275039--immunoprecipitation-img5750.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-C10 antibody [EPR23475-105] - BSA and Azide free (AB275039)")
![Flow Cytometry (Intracellular) - Anti-C10 antibody [EPR23475-105] - BSA and Azide free (AB275039), expandable thumbnail](https://content.abcam.com/products/images/c10-antibody-epr23475-105-bsa-and-azide-free-ab275039--flow-cytometry-intracellular-img38384.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-C10 antibody [EPR23475-105] - BSA and Azide free (AB275039)")
![Western blot - Anti-C10 antibody [EPR23475-105] - BSA and Azide free (AB275039), expandable thumbnail](https://content.abcam.com/products/images/c10-antibody-epr23475-105-bsa-and-azide-free-ab275039--western-blot-img37003.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-C10 antibody [EPR23475-105] - BSA and Azide free (AB275039)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: PBS
- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-Fr | Flow Cyt (Intra) | IHC-P | ICC/IF | |
|---|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Tested | Not recommended | Tested | Tested | Tested | Not recommended |
| Rat | Expected | Not recommended | Not recommended | Expected | Not recommended | Not recommended |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Does not give a signal with endogenous expression samples. |
Species Human | Dilution info - | Notes Does not give a signal with endogenous expression samples. |
Species Rat | Dilution info - | Notes Does not give a signal with endogenous expression samples. |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). |
Species Human | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Target data
Function
Chemotactic factor that attracts mostly macrophage, but it can also attract B cells, CD4(+) lymphocytes and eosinophils.
Alternative names
C10, Scya6, Ccl6, C-C motif chemokine 6, Protein C10, Small-inducible cytokine A6
Recommended products
Rabbit Recombinant Monoclonal C10 antibody. Carrier free. Suitable for IP, IHC-Fr, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: PBS
- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR23475-105
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab275039 is the carrier-free version of Anti-C10 antibody [EPR23475-105] ab275025.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
C10 also known as complement component C10 plays an important role in the immune system by facilitating cell lysis through the membrane attack complex (MAC) assembly. It is part of the complement cascade traditionally involved in destroying pathogens and infected host cells. The protein has a molecular mass of approximately 30 kilodaltons. C10 expression occurs mainly in liver hepatocytes but its product circulates in the bloodstream and can also be found in various body fluids.
- Biological function summary
This complement component carries out an essential function in innate immunity by contributing to the execution of cellular apoptosis and pathogen elimination. C10 becomes a part of the larger MAC complex interacting with other complement proteins such as C5b C6 C7 C8 and C9 to form pores on target cell membranes. These pore formations help in creating a passage for ions and molecules leading to osmotic lysis and subsequent cell death integral for immune defense.
- Pathways
C10 integrates into the complement activation pathway particularly within the terminal pathway of the complement system. This pathway involves a cascade of protein interactions initiated by different triggers such as antigen-antibody complexes or pathogen surfaces. Related proteins like C3 and C5 facilitate the formation and activation of MAC focusing on pathogen neutralization. C10's mechanistic role ensures the effective completion of this terminal pathway contributing significantly to both classical and alternative complement pathways.
- Associated diseases and disorders
Abnormalities in C10 activity can relate to conditions such as paroxysmal nocturnal hemoglobinuria (PNH) and systemic lupus erythematosus (SLE). In PNH faulty complement regulation due to mutations in complement regulatory proteins like CD55 and CD59 leads to red blood cell destruction where C10's role in MAC formation aggravates hemolysis. In SLE autoantibody production and complement activation create immune complexes that accumulate in organs exacerbating inflammation and tissue damage. Understanding C10's interaction with these proteins provides insight into the therapeutic potential for managing such autoimmune-related conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C10 antibody [EPR23475-105] - BSA and Azide free (ab275039), expandable thumbnail](https://content.abcam.com/products/images/c10-antibody-epr23475-105-bsa-and-azide-free-ab275039--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img112432.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C10 antibody [EPR23475-105] - BSA and Azide free (ab275039)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C10 antibody [EPR23475-105] - BSA and Azide free (ab275039)
This data was developed using Anti-C10 antibody [EPR23475-105] ab275025, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling C10 with Anti-C10 antibody [EPR23475-105] ab275025 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Postive staining on mouse spleen (PMID: 15770051). The section was incubated with Anti-C10 antibody [EPR23475-105] ab275025 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
![Immunohistochemistry (Frozen sections) - Anti-C10 antibody [EPR23475-105] - BSA and Azide free (ab275039), expandable thumbnail](https://content.abcam.com/products/images/c10-antibody-epr23475-105-bsa-and-azide-free-ab275039--immunohistochemistry-frozen-sections-img60806.jpg "Immunohistochemistry (Frozen sections) - Anti-C10 antibody [EPR23475-105] - BSA and Azide free (ab275039)")
Immunohistochemistry (Frozen sections) - Anti-C10 antibody [EPR23475-105] - BSA and Azide free (ab275039)
This data was developed using Anti-C10 antibody [EPR23475-105] ab275025, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse small intestine tissue labeling C10 with Anti-C10 antibody [EPR23475-105] ab275025 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution (Green). Positive staining on mouse small intestine (PMID: 7579352). The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution.
![Immunoprecipitation - Anti-C10 antibody [EPR23475-105] - BSA and Azide free (ab275039), expandable thumbnail](https://content.abcam.com/products/images/c10-antibody-epr23475-105-bsa-and-azide-free-ab275039--immunoprecipitation-img5750.jpg "Immunoprecipitation - Anti-C10 antibody [EPR23475-105] - BSA and Azide free (ab275039)")
Immunoprecipitation - Anti-C10 antibody [EPR23475-105] - BSA and Azide free (ab275039)
This data was developed using Anti-C10 antibody [EPR23475-105] ab275025, the same antibody clone in a different buffer formulation.
C10 was immunoprecipitated from 0.35 mg Mouse small intestine tissue lysate with Anti-C10 antibody [EPR23475-105] ab275025 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-C10 antibody [EPR23475-105] ab275025 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/1000 dilution.
Lane 1: Mouse small intestine tissue lysate 10 ug
Lane 2: Anti-C10 antibody [EPR23475-105] ab275025 IP in Mouse small intestine tissue lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-C10 antibody [EPR23475-105] ab275025 in mouse small intestine tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
The expression profile observed is consistent with what has been described in the literature (PMID: 19812544).
All lanes: Immunoprecipitation - Anti-C10 antibody [EPR23475-105] (Anti-C10 antibody [EPR23475-105] ab275025)
Predicted band size: 13 kDa
Observed band size: 13 kDa
![Flow Cytometry (Intracellular) - Anti-C10 antibody [EPR23475-105] - BSA and Azide free (ab275039), expandable thumbnail](https://content.abcam.com/products/images/c10-antibody-epr23475-105-bsa-and-azide-free-ab275039--flow-cytometry-intracellular-img38384.jpg "Flow Cytometry (Intracellular) - Anti-C10 antibody [EPR23475-105] - BSA and Azide free (ab275039)")
Flow Cytometry (Intracellular) - Anti-C10 antibody [EPR23475-105] - BSA and Azide free (ab275039)
This data was developed using Anti-C10 antibody [EPR23475-105] ab275025, the same antibody clone in a different buffer formulation.
Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized P388D1 (Mouse lymphoma monocyte; macrophage) (treated with 1ug/ml lipopolysaccharide (LPS) for 4h then add 300ng/ml Brefeldin A for 16h) (Red) / Untreated control (Green) cells labelling C10 with Anti-C10 antibody [EPR23475-105] ab275025 at 1/500 dilution (0.1ug) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
A Goat anti rabbit IgG (Alexa Fluor®488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.
![Western blot - Anti-C10 antibody [EPR23475-105] - BSA and Azide free (ab275039), expandable thumbnail](https://content.abcam.com/products/images/c10-antibody-epr23475-105-bsa-and-azide-free-ab275039--western-blot-img37003.jpg "Western blot - Anti-C10 antibody [EPR23475-105] - BSA and Azide free (ab275039)")
Western blot - Anti-C10 antibody [EPR23475-105] - BSA and Azide free (ab275039)
This data was developed using Anti-C10 antibody [EPR23475-105] ab275025, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 8 seconds.
This product does not recognize CCL9.
All lanes: Western blot - Anti-C10 antibody [EPR23475-105] (Anti-C10 antibody [EPR23475-105] ab275025) at 1/1000 dilution
Lane 1: His-tagged mouse C10 recombinant protein, 20ng
Lane 2: His-tagged mouse MIP-1 gamma (CCL9) recombinant protein (aa 22-122), 50ng
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 13 kDa
![Immunohistochemistry (Frozen sections) - Anti-C10 antibody [EPR23475-105] - BSA and Azide free (ab275039), expandable thumbnail](https://content.abcam.com/products/images/c10-antibody-epr23475-105-bsa-and-azide-free-ab275039--immunohistochemistry-frozen-sections-img134378.jpg "Immunohistochemistry (Frozen sections) - Anti-C10 antibody [EPR23475-105] - BSA and Azide free (ab275039)")
Immunohistochemistry (Frozen sections) - Anti-C10 antibody [EPR23475-105] - BSA and Azide free (ab275039)
This data was developed using Anti-C10 antibody [EPR23475-105] ab275025, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse colon tissue labeling C10 with Anti-C10 antibody [EPR23475-105] ab275025 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution (Green). Positive staining on mouse colon (PMID: 7579352). The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 (2 ug/ml) dilution.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C10 antibody [EPR23475-105] - BSA and Azide free (ab275039), expandable thumbnail](https://content.abcam.com/products/images/c10-antibody-epr23475-105-bsa-and-azide-free-ab275039--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img38919.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C10 antibody [EPR23475-105] - BSA and Azide free (ab275039)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C10 antibody [EPR23475-105] - BSA and Azide free (ab275039)
This data was developed using Anti-C10 antibody [EPR23475-105] ab275025, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling C10 with Anti-C10 antibody [EPR23475-105] ab275025 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Postive staining on mouse lung (PMID: 14734772). The section was incubated with Anti-C10 antibody [EPR23475-105] ab275025 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
![Immunohistochemistry (Frozen sections) - Anti-C10 antibody [EPR23475-105] - BSA and Azide free (ab275039), expandable thumbnail](https://content.abcam.com/products/images/c10-antibody-epr23475-105-bsa-and-azide-free-ab275039--immunohistochemistry-frozen-sections-img134378.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-C10 antibody [EPR23475-105] - BSA and Azide free (ab275039)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C10 antibody [EPR23475-105] - BSA and Azide free (ab275039), expandable thumbnail](https://content.abcam.com/products/images/c10-antibody-epr23475-105-bsa-and-azide-free-ab275039--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img38919.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C10 antibody [EPR23475-105] - BSA and Azide free (ab275039)")
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