AB189923

Anti-C10orf35 antibody [EP14950-82]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal C10orf35 antibody. Suitable for IP, WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

C10orf35, FAM241B, Protein FAM241B

3 Images

Supplier Data

Immunoprecipitation - Anti-C10orf35 antibody [EP14950-82] (AB189923)

Western blot analysis of C10orf35 in Human fetal brain lysate immunoprecipitated using ab189923 at 1/50 dilution.
Secondary : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.

All lanes:

Immunoprecipitation - Anti-C10orf35 antibody [EP14950-82] (ab189923)

Predicted band size: 13 kDa

false

Supplier Data

Western blot - Anti-C10orf35 antibody [EP14950-82] (AB189923)

All lanes:

Western blot - Anti-C10orf35 antibody [EP14950-82] (ab189923) at 1/50000 dilution

Lane 1:

Human fetal brain lysate at 20 µg

Lane 2:

Human fetal kidney lysate at 20 µg

Lane 3:

MCF7 lysate at 20 µg

Lane 4:

A431 lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 13 kDa

Observed band size: 13 kDa

false

Supplier Data

Western blot - Anti-C10orf35 antibody [EP14950-82] (AB189923)

All lanes:

Western blot - Anti-C10orf35 antibody [EP14950-82] (ab189923) at 1/10000 dilution

Lane 1:

Mouse brain lysate at 10 µg

Lane 2:

Rat brain lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 13 kDa

Observed band size: 13 kDa

false

Carrier free

Anti-C10orf35 antibody [EP14950-82] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP14950-82

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/50 - 1/70", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

C10orf35 also known as Chromosome 10 Open Reading Frame 35 encodes a protein with a mass of approximately 86 kDa. This protein primarily localizes to the cytoplasm and shows significant expression in various tissues including the testis brain and heart. Its exact mechanical role remains not fully understood but it is suggested to be involved in cellular processes related to structural organization.

Biological function summary

C10orf35 interacts with cellular components often associated with maintaining cellular architecture and signaling pathways. It does not typically form part of well-defined protein complexes yet it still contributes to the integrity and function of specialized cells. The expression pattern hints at a mechanism influencing cell differentiation and proliferation particularly in reproductive and neural tissues.

Pathways

Researchers have linked C10orf35 to significant cellular signaling and development pathways including the Wnt/β-catenin signaling pathway and the Hedgehog signaling pathway. These pathways play critical roles in cell fate determination and embryonic development. C10orf35 shows interaction with proteins like β-catenin and Hedgehog influencing downstream signaling events critical for transcriptional regulation and morphogenetic processes.

Researchers investigated the involvement of this target in developmental disorders and cancers particularly testicular cancer. The malfunction or abnormal expression of C10orf35 can disrupt cellular homeostasis leading to uncontrolled cell proliferation and differentiation. Its interaction with β-catenin is particularly notable in cancer-related research suggesting potential as a therapeutic target for modulating oncogenic pathways.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May play a role in lysosome homeostasis.

See full target information FAM241B

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:5481 PubMed40593590

2025

Single-cell analysis reveals a longitudinal trajectory of meningioma evolution and heterogeneity.

Applications

Unspecified application

Species

Unspecified reactive species

Ji Yoon Lee,Eun Jung Lee,Bo Yeon Seo,Jiwon Kim,Youjin Song,Dayoung Lee,Namsung Moon,Harim Koo,Chul-Kee Park,Min-Sung Kim,Serk In Park,Do-Hyun Nam,Doo-Sik Kong,Jason K Sa

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-C10orf35 antibody [EP14950-82]

Immunoprecipitation - Anti-C10orf35 antibody [EP14950-82] (AB189923)

Western blot - Anti-C10orf35 antibody [EP14950-82] (AB189923)

Western blot - Anti-C10orf35 antibody [EP14950-82] (AB189923)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise