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AB284845

Anti-C10orf35 antibody [EP14950-82] - BSA and Azide free

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Rabbit Recombinant Monoclonal C10orf35 antibody. Carrier free. Suitable for IP, WB and reacts with Human, Mouse, Rat samples.

View Alternative Names

C10orf35, FAM241B, Protein FAM241B

3 Images
Western blot - Anti-C10orf35 antibody [EP14950-82] - BSA and Azide free (AB284845)
  • WB

Supplier Data

Western blot - Anti-C10orf35 antibody [EP14950-82] - BSA and Azide free (AB284845)

This data was developed using ab189923, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-C10orf35 antibody [EP14950-82] (<a href='/en-us/products/primary-antibodies/c10orf35-antibody-ep14950-82-ab189923'>ab189923</a>) at 1/50000 dilution

Lane 1:

Human fetal brain lysate at 20 µg

Lane 2:

Human fetal kidney lysate at 20 µg

Lane 3:

MCF7 lysate at 20 µg

Lane 4:

A431 lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 13 kDa

Observed band size: 13 kDa

false

Western blot - Anti-C10orf35 antibody [EP14950-82] - BSA and Azide free (AB284845)
  • WB

Supplier Data

Western blot - Anti-C10orf35 antibody [EP14950-82] - BSA and Azide free (AB284845)

This data was developed using ab189923, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-C10orf35 antibody [EP14950-82] (<a href='/en-us/products/primary-antibodies/c10orf35-antibody-ep14950-82-ab189923'>ab189923</a>) at 1/10000 dilution

Lane 1:

Mouse brain lysate at 10 µg

Lane 2:

Rat brain lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 13 kDa

Observed band size: 13 kDa

false

Immunoprecipitation - Anti-C10orf35 antibody [EP14950-82] - BSA and Azide free (AB284845)
  • IP

Supplier Data

Immunoprecipitation - Anti-C10orf35 antibody [EP14950-82] - BSA and Azide free (AB284845)

This data was developed using ab189923, the same antibody clone in a different buffer formulation.Western blot analysis of C10orf35 in Human fetal brain lysate immunoprecipitated using ab189923 at 1/50 dilution.
Secondary : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.

All lanes:

Immunoprecipitation - Anti-C10orf35 antibody [EP14950-82] (<a href='/en-us/products/primary-antibodies/c10orf35-antibody-ep14950-82-ab189923'>ab189923</a>)

Predicted band size: 13 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP14950-82

Isotype

IgG

Carrier free

Yes

Reacts with

Rat, Human, Mouse

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab284845 is the carrier-free version of ab189923

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: 100% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

C10orf35 also known as Chromosome 10 Open Reading Frame 35 encodes a protein with a mass of approximately 86 kDa. This protein primarily localizes to the cytoplasm and shows significant expression in various tissues including the testis brain and heart. Its exact mechanical role remains not fully understood but it is suggested to be involved in cellular processes related to structural organization.
Biological function summary

C10orf35 interacts with cellular components often associated with maintaining cellular architecture and signaling pathways. It does not typically form part of well-defined protein complexes yet it still contributes to the integrity and function of specialized cells. The expression pattern hints at a mechanism influencing cell differentiation and proliferation particularly in reproductive and neural tissues.

Pathways

Researchers have linked C10orf35 to significant cellular signaling and development pathways including the Wnt/β-catenin signaling pathway and the Hedgehog signaling pathway. These pathways play critical roles in cell fate determination and embryonic development. C10orf35 shows interaction with proteins like β-catenin and Hedgehog influencing downstream signaling events critical for transcriptional regulation and morphogenetic processes.

Researchers investigated the involvement of this target in developmental disorders and cancers particularly testicular cancer. The malfunction or abnormal expression of C10orf35 can disrupt cellular homeostasis leading to uncontrolled cell proliferation and differentiation. Its interaction with β-catenin is particularly notable in cancer-related research suggesting potential as a therapeutic target for modulating oncogenic pathways.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May play a role in lysosome homeostasis.
See full target information FAM241B
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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