AB235362

Anti-C10orf54 antibody

Name: Anti-C10orf54 antibody (ab235362) | Abcam
Brand: Abcam Limited
SKU: AB235362
Price: 525 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal VISTA antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human VSIR aa 1-200.

View Alternative Names

C10orf54, SISP1, VISTA, PP2135, UNQ730/PRO1412, VSIR, V-type immunoglobulin domain-containing suppressor of T-cell activation, Platelet receptor Gi24, Stress-induced secreted protein-1, V-set domain-containing immunoregulatory receptor, V-set immunoregulatory receptor, Sisp-1

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C10orf54 antibody (AB235362)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C10orf54 antibody (AB235362)

Paraffin-embedded human adrenal gland tissue stained for C10orf54 using ab235362 at 1/100 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-C10orf54 antibody (AB235362)

All lanes:

Western blot - Anti-C10orf54 antibody (ab235362) at 1/1000 dilution

All lanes:

Mouse liver tissue lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/10000 dilution

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human VSIR aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

Q9H7M9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purity >95%

Storage buffer

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

C10orf54 also known as VISTA (V-domain Ig suppressor of T cell activation) or PD-1H is a protein found on the surface of immune cells. It has a mass of approximately 50-55 kDa and shows expression mainly in hematopoietic tissues including myeloid cells and tissues of the immune system. This protein acts as a negative immune checkpoint regulator playing a role in modulating immune responses and maintaining immune homeostasis.

Biological function summary

C10orf54 affects the activity of T cells significantly. It functions by interacting with T cell receptors to inhibit excessive immune responses. Although not part of a larger complex C10orf54 modulates immune cell interactions on its own influencing the balance between co-stimulatory and inhibitory signals that control T cell activation and proliferation.

Pathways

C10orf54 integrates into the immune regulation processes within the immune checkpoint pathways and T cell receptor signaling pathway. It closely relates to proteins such as PD-1 and CTLA-4 which are other known immune checkpoint regulators. These pathways involve critical control points for preventing autoimmunity and regulating immune tolerance.

C10orf54 associates particularly with cancer and autoimmune disorders. Its involvement in cancer arises from its ability to suppress anti-tumor immune responses often making tumors more resistant to immune-mediated destruction. As an immune checkpoint its expression can also link to autoimmune diseases where it modulates unchecked immune activation. In these contexts C10orf54 interplays with proteins like PD-L1 to affect the dynamics of immune evasion and tolerance within disease states.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Immunoregulatory receptor which inhibits the T-cell response (PubMed : 24691993). May promote differentiation of embryonic stem cells, by inhibiting BMP4 signaling (By similarity). May stimulate MMP14-mediated MMP2 activation (PubMed : 20666777).

See full target information VSIR

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of personalized medicine 15: PubMed40137423

2025

Dysregulation of Circadian Markers, HAT1 and Associated Epigenetic Proteins, and the Anti-Aging Protein KLOTHO in Placenta of Pregnant Women with Chronic Venous Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Oscar Fraile-Martinez,Cielo García-Montero,Tatiana Pekarek,Julia Bujan,Silvestra Barrena-Blázquez,Eva Manuela Pena-Burgos,Laura López-González,Leonel Pekarek,Raul Díaz-Pedrero,Juan A De León-Luis,Coral Bravo,Melchor Álvarez-Mon,Miguel A Saez,Natalio García-Honduvilla,Miguel A Ortega

Journal of clinical medicine 10: PubMed33925488

2021

Combined Inhibition of TGF-β1-Induced EMT and PD-L1 Silencing Re-Sensitizes Hepatocellular Carcinoma to Sorafenib Treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Ritu Shrestha,Prashanth Prithviraj,Kim R Bridle,Darrell H G Crawford,Aparna Jayachandran

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-C10orf54 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C10orf54 antibody (AB235362)

Western blot - Anti-C10orf54 antibody (AB235362)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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