Rabbit Polyclonal C12orf34 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human FAM222A aa 150-300.
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- Intractable & rare diseases research 9:217-2212020Reactive astrocytes express Aggregatin () in the brains of Alzheimer's disease and Nasu-Hakola disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJun-Ichi Satoh,Yoshihiro Kino,Motoaki Yanaizu,Tsuyoshi Ishida,Yuko SaitoPubMed 33139980
- Nature communications 11:4112020FAM222A encodes a protein which accumulates in plaques in Alzheimer's disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTingxiang Yan,Jingjing Liang,Ju Gao,Luwen Wang,Hisashi Fujioka,Xiaofeng Zhu,Xinglong WangPubMed 31964863
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human FAM222A aa 150-300. The exact immunogen used to generate this antibody is proprietary information. Database link Q5U5X8
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes - |
Target data
Alternative names
C12orf34, FAM222A, Protein FAM222A
Recommended products
Rabbit Polyclonal C12orf34 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human FAM222A aa 150-300.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human FAM222A aa 150-300. The exact immunogen used to generate this antibody is proprietary information. Database link Q5U5X8
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
C12orf34 also known as chromosome 12 open reading frame 34 is a protein coding gene with a molecular mass of approximately 53 kDa. The protein expression of C12orf34 shows tissue-specific patterns prominently in the brain skeletal muscle and liver. This suggests its involvement in performing essential functions in these particular tissues. Notably it exhibits a unique sequence composition which may result in specific signaling or structural roles though precise mechanical actions remain under investigation.
- Biological function summary
This protein appears to function as part of a larger protein complex indicating a collaborative role in intracellular processes. C12orf34 has been implicated in the regulation of signaling pathways that affect cellular growth and metabolism suggesting its importance in maintaining cellular homeostasis. Its activity may influence gene expression and protein synthesis connecting it to broader cellular functions within those systems where it is expressed.
- Pathways
This protein acts within tightly regulated metabolic and signaling processes. It is associated with key pathways such as the PI3K-Akt pathway which is critical for regulating cell survival and growth. Through this pathway C12orf34 interacts with other proteins like mTOR a central regulator of cell metabolism. By participating in these pathways C12orf34 helps integrate external signals with cellular responses linking environmental cues with cellular growth and energy balance.
- Associated diseases and disorders
Researchers associate C12orf34 with conditions such as muscular dystrophy and neurodegenerative diseases. Its connection to these disorders suggests that mutations or dysregulation in C12orf34 or its associated pathways can disrupt normal cellular functions. Additionally the interaction of C12orf34 with proteins related to neurological health such as those in the Akt signaling pathway further supports its potential role in the onset or progression of these conditions. Understanding these links may offer insights into novel therapeutic strategies for related diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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Full details and terms and conditions can be found here:
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6 product images
Immunocytochemistry/ Immunofluorescence - Anti-C12orf34 antibody (ab122626)
Immunofluorescent staining of Human cell line U-251 MG shows positivity in nucleus but not nucleoli, plasma membrane, mitochondria and focal adhesions. Recommended concentration of ab122626 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Western blot - Anti-C12orf34 antibody (ab122626)
All lanes: Western blot - Anti-C12orf34 antibody (ab122626)
All lanes: SH-SY5Y (human neuroblastoma cell line from bone marrow) whole cell lysate
Predicted band size: 47 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C12orf34 antibody (ab122626)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of stomach tissue labelling C12orf34 with ab122626 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C12orf34 antibody (ab122626)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of cerebral cortex tissue labelling C12orf34 with ab122626 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C12orf34 antibody (ab122626)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of testis tissue labelling C12orf34 with ab122626 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C12orf34 antibody (ab122626)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of skeletal muscle tissue labelling C12orf34 with ab122626 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol. No positivity in myocytes as expected.
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