AB122626

Anti-C12orf34 antibody

Name: Anti-C12orf34 antibody (ab122626) | Abcam
Brand: Abcam Limited
SKU: AB122626

Be the first to review this product! Submit a review

(3 Publications)

Rabbit Polyclonal C12orf34 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human FAM222A aa 150-300.

View Alternative Names

C12orf34, FAM222A, Protein FAM222A

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C12orf34 antibody (AB122626)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C12orf34 antibody (AB122626)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of cerebral cortex tissue labelling C12orf34 with ab122626 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C12orf34 antibody (AB122626)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of stomach tissue labelling C12orf34 with ab122626 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C12orf34 antibody (AB122626)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of testis tissue labelling C12orf34 with ab122626 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-C12orf34 antibody (AB122626)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-C12orf34 antibody (AB122626)

Immunofluorescent staining of Human cell line U-251 MG shows positivity in nucleus but not nucleoli, plasma membrane, mitochondria and focal adhesions. Recommended concentration of ab122626 1-4 µg/ml. Cells treated with PFA/Triton X-100.

Supplier Data

Western blot - Anti-C12orf34 antibody (AB122626)

All lanes:

Western blot - Anti-C12orf34 antibody (ab122626)

All lanes:

SH-SY5Y (human neuroblastoma cell line from bone marrow) whole cell lysate

Predicted band size: 47 kDa

false

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C12orf34 antibody (AB122626)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of skeletal muscle tissue labelling C12orf34 with ab122626 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol. No positivity in myocytes as expected.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human FAM222A aa 150-300. The exact immunogen used to generate this antibody is proprietary information.

Q5U5X8

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

C12orf34 also known as chromosome 12 open reading frame 34 is a protein coding gene with a molecular mass of approximately 53 kDa. The protein expression of C12orf34 shows tissue-specific patterns prominently in the brain skeletal muscle and liver. This suggests its involvement in performing essential functions in these particular tissues. Notably it exhibits a unique sequence composition which may result in specific signaling or structural roles though precise mechanical actions remain under investigation.

Biological function summary

This protein appears to function as part of a larger protein complex indicating a collaborative role in intracellular processes. C12orf34 has been implicated in the regulation of signaling pathways that affect cellular growth and metabolism suggesting its importance in maintaining cellular homeostasis. Its activity may influence gene expression and protein synthesis connecting it to broader cellular functions within those systems where it is expressed.

Pathways

This protein acts within tightly regulated metabolic and signaling processes. It is associated with key pathways such as the PI3K-Akt pathway which is critical for regulating cell survival and growth. Through this pathway C12orf34 interacts with other proteins like mTOR a central regulator of cell metabolism. By participating in these pathways C12orf34 helps integrate external signals with cellular responses linking environmental cues with cellular growth and energy balance.

Researchers associate C12orf34 with conditions such as muscular dystrophy and neurodegenerative diseases. Its connection to these disorders suggests that mutations or dysregulation in C12orf34 or its associated pathways can disrupt normal cellular functions. Additionally the interaction of C12orf34 with proteins related to neurological health such as those in the Akt signaling pathway further supports its potential role in the onset or progression of these conditions. Understanding these links may offer insights into novel therapeutic strategies for related diseases.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

See full target information FAM222A

style

left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Translational neurodegeneration 13:24 PubMed38671492

2024

Agomirs upregulating carboxypeptidase E expression rescue hippocampal neurogenesis and memory deficits in Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Dongfang Jiang,Hongmei Liu,Tingting Li,Song Zhao,Keyan Yang,Fuwen Yao,Bo Zhou,Haiping Feng,Sijia Wang,Jiaqi Shen,Jinglan Tang,Yu-Xin Zhang,Yun Wang,Caixia Guo,Tie-Shan Tang

Intractable & rare diseases research 9:217-221 PubMed33139980

2020

Reactive astrocytes express Aggregatin () in the brains of Alzheimer's disease and Nasu-Hakola disease.

Applications

Unspecified application

Species

Unspecified reactive species

Jun-Ichi Satoh,Yoshihiro Kino,Motoaki Yanaizu,Tsuyoshi Ishida,Yuko Saito

Nature communications 11:411 PubMed31964863

2020

FAM222A encodes a protein which accumulates in plaques in Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Tingxiang Yan,Jingjing Liang,Ju Gao,Luwen Wang,Hisashi Fujioka,Xiaofeng Zhu,Xinglong Wang

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-C12orf34 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C12orf34 antibody (AB122626)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C12orf34 antibody (AB122626)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C12orf34 antibody (AB122626)

Immunocytochemistry/ Immunofluorescence - Anti-C12orf34 antibody (AB122626)

Western blot - Anti-C12orf34 antibody (AB122626)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C12orf34 antibody (AB122626)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise