Rabbit Recombinant Monoclonal C12orf35 antibody. Suitable for WB and reacts with Mouse samples.
Images
![Western blot - Anti-C12orf35 antibody [EPR28486-77] (AB323850), expandable thumbnail](https://content.abcam.com/products/images/c12orf35-antibody-epr28486-77-ab323850--western-blot-img447002.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-C12orf35 antibody [EPR28486-77] (AB323850)")
![Western blot - Anti-C12orf35 antibody [EPR28486-77] (AB323850), expandable thumbnail](https://content.abcam.com/products/images/c12orf35-antibody-epr28486-77-ab323850--western-blot-img446962.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-C12orf35 antibody [EPR28486-77] (AB323850)")
![Western blot - Anti-C12orf35 antibody [EPR28486-77] (AB323850), expandable thumbnail](https://content.abcam.com/products/images/c12orf35-antibody-epr28486-77-ab323850--western-blot-img446961.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-C12orf35 antibody [EPR28486-77] (AB323850)")
![Western blot - Anti-C12orf35 antibody [EPR28486-77] (AB323850), expandable thumbnail](https://content.abcam.com/products/images/c12orf35-antibody-epr28486-77-ab323850--western-blot-img446960.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-C12orf35 antibody [EPR28486-77] (AB323850)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IP | IHC-P | ICC/IF | |
|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Tested | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
Target data
Alternative names
Kiaa1551, C12orf35, Retroelement silencing factor 1
Rabbit Recombinant Monoclonal C12orf35 antibody. Suitable for WB and reacts with Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR28486-77
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-C12orf35 antibody [EPR28486-77] (ab323850), expandable thumbnail](https://content.abcam.com/products/images/c12orf35-antibody-epr28486-77-ab323850--western-blot-img447002.jpg "Western blot - Anti-C12orf35 antibody [EPR28486-77] (ab323850)")
Western blot - Anti-C12orf35 antibody [EPR28486-77] (ab323850)
C12orf35 Western blot staining using rabbit Anti-C12orf35 antibody
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
To minimize protein degradation, cells/tissues were lysed immediately after harvest and run directly on a gel.
The identity of the lower MW band at approximately 75 kDa (in lane 1) is unknown.
Exposure time: Lane 1: 81 seconds, Lane 2: 180 seconds
All lanes: Western blot - Anti-C12orf35 antibody [EPR28486-77] (ab323850) at 1/1000 dilution
Lane 1: ES-D3 [D3] (mouse blastocyst-derived embryonic stem cell) treated with 10 uM MG-132 for 24 hours whole cell lysate at 20 µg
Lane 2: Mouse ovary tissue lysate at 40 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 250 kDa
![Western blot - Anti-C12orf35 antibody [EPR28486-77] (ab323850), expandable thumbnail](https://content.abcam.com/products/images/c12orf35-antibody-epr28486-77-ab323850--western-blot-img446962.jpg "Western blot - Anti-C12orf35 antibody [EPR28486-77] (ab323850)")
Western blot - Anti-C12orf35 antibody [EPR28486-77] (ab323850)
C12orf35 Western blot staining of Mouse E18 brain tissue lysate using rabbit Anti-C12orf35 antibody
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
To minimize protein degradation, tissues were lysed immediately after harvest and run directly on a gel.
The identity of the bands between 20 kDa and 60 kDa are unknown.
All lanes: Western blot - Anti-C12orf35 antibody [EPR28486-77] (ab323850) at 1/1000 dilution
All lanes: Mouse E18 brain tissue lysate at 50 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 250 kDa
Exposure time: 59s
![Western blot - Anti-C12orf35 antibody [EPR28486-77] (ab323850), expandable thumbnail](https://content.abcam.com/products/images/c12orf35-antibody-epr28486-77-ab323850--western-blot-img446961.jpg "Western blot - Anti-C12orf35 antibody [EPR28486-77] (ab323850)")
Western blot - Anti-C12orf35 antibody [EPR28486-77] (ab323850)
C12orf35 Western blot staining using rabbit Anti-C12orf35 antibody
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
To minimize protein degradation, tissues were lysed immediately after harvest and run directly on a gel.
All lanes: Western blot - Anti-C12orf35 antibody [EPR28486-77] (ab323850) at 1/1000 dilution
Lane 1: Mouse testis tissue lysate at 50 µg
Lane 2: Mouse lung tissue lysate at 50 µg
Lane 3: Mouse E15 brain tissue lysate at 50 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 250 kDa
Exposure time: 180s
![Western blot - Anti-C12orf35 antibody [EPR28486-77] (ab323850), expandable thumbnail](https://content.abcam.com/products/images/c12orf35-antibody-epr28486-77-ab323850--western-blot-img446960.jpg "Western blot - Anti-C12orf35 antibody [EPR28486-77] (ab323850)")
Western blot - Anti-C12orf35 antibody [EPR28486-77] (ab323850)
C12orf35 Western blot staining using rabbit Anti-C12orf35 antibody
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
This image was kindly provided by an anonymous collaborator.
The identity of the bands between 20 kDa and 60 kDa are unknown.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-C12orf35 antibody [EPR28486-77] (ab323850) at 1/1000 dilution
Lane 1: Wild-type mouse kidney tissue lysate
Lane 2: RESF1 knockout mouse kidney tissue lysate
Observed band size: 250 kDa, 36 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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