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AB122448

Anti-C12orf65 antibody

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(2 Publications)

Rabbit Polyclonal C12orf65 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MTRFR aa 1-100.

View Alternative Names

C12orf65, My030, MTRFR, Mitochondrial translation release factor in rescue

1 Images
Western blot - Anti-C12orf65 antibody (AB122448)
  • WB

Supplier Data

Western blot - Anti-C12orf65 antibody (AB122448)

All lanes:

Western blot - Anti-C12orf65 antibody (ab122448) at 0.4 µg/mL

All lanes:

SCLC-21H cell lysate

Predicted band size: 19 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human MTRFR aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

Q9H3J6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

C12orf65 also known as Chromosome 12 Open Reading Frame 65 is a protein that plays roles in mitochondrial translation. It weighs approximately 21 kDa. This protein is expressed mainly in the mitochondria of various tissues indicating its importance in cellular energy processes. Researchers study C12orf65 for understanding mitochondrial-related functions and disorders.
Biological function summary

C12orf65 is involved in the termination of mitochondrial translation. It works as a release factor part of the protein synthesis machinery within the mitochondria. This highlights its participation in a complex required for translating mitochondrial-encoded proteins necessary for cellular respiration. Still further research helps clarify more intricate aspects of its biological role.

Pathways

C12orf65 participates in pathways connected to mitochondrial biogenesis and oxidative phosphorylation. It shares an association with proteins such as mtRF1 and mtRF1a which participate within similar pathways supporting its role in efficient mitochondrial function. This involvement ties back to the production of ATP a critical energy currency of the cell.

Disruptions in C12orf65 function can lead to mitochondrial disorders notably combined oxidative phosphorylation deficiency. This points to impaired energy metabolism at the cellular level. C12orf65 links to other mitochondrial proteins potentially influencing disorders characterized by deficient energy production offering insights into potential treatment strategies for affected individuals.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Part of a mitoribosome-associated quality control pathway that prevents aberrant translation by responding to interruptions during elongation (PubMed : 33243891). As heterodimer with MTRES1, ejects the unfinished nascent chain and peptidyl transfer RNA (tRNA), respectively, from stalled ribosomes. Recruitment of mitoribosome biogenesis factors to these quality control intermediates suggests additional roles for MTRES1 and MTRF during mitoribosome rescue (PubMed : 33243891).
See full target information MTRFR
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

EMBO reports 24:e57228 PubMed37818824

2023

Tetracyclines activate mitoribosome quality control and reduce ER stress to promote cell survival.

Applications

Unspecified application

Species

Unspecified reactive species

Conor T Ronayne,Thomas D Jackson,Christopher F Bennett,Elizabeth A Perry,Noa Kantorovic,Pere Puigserver

Scientific reports 7:42060 PubMed28169357

2017

Integrative analysis of copy number and transcriptional expression profiles in esophageal cancer to identify a novel driver gene for therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Gaochao Dong,Qixing Mao,Decai Yu,Yi Zhang,Mantang Qiu,Gaoyue Dong,Qiang Chen,Wenjie Xia,Jie Wang,Lin Xu,Feng Jiang
View all publications
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