AB125084

Anti-C14orf133 antibody

Name: Anti-C14orf133 antibody (ab125084) | Abcam
Brand: Abcam Limited
SKU: AB125084

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(4 Publications)

Rabbit Polyclonal C14orf133 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human VIPAS39 aa 400 to C-terminus.

View Alternative Names

C14orf133, SPE39, VIPAR, VIPAS39, Spermatogenesis-defective protein 39 homolog, hSPE-39, VPS33B-interacting protein in apical-basolateral polarity regulator, VPS33B-interacting protein in polarity and apical restriction

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Immunoprecipitation - Anti-C14orf133 antibody (AB125084)

Detection of C14orf133 by Western Blot of Immunprecipitate.
ab125084 at 1µg/ml staining C14orf133 in HeLa whole cell lysate immunoprecipitated using ab125084 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection : Chemiluminescence with exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-C14orf133 antibody (ab125084)

Predicted band size: 57 kDa

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Western blot - Anti-C14orf133 antibody (AB125084)

All lanes:

Western blot - Anti-C14orf133 antibody (ab125084) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

293T whole cell lysate at 50 µg

Lane 4:

Jurkat whole cell lysate at 50 µg

Lane 5:

NIH3T3 whole cell lysate at 50 µg

Predicted band size: 57 kDa

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Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Mouse

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human VIPAS39 aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9H9C1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab125084 was affinity purified using an epitope specific to C14orf133 immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The C14orf133 protein also known as chromosome 14 open reading frame 133 is an intriguing target in biological research. This protein has a molecular mass of approximately 45 kDa. C14orf133 expression is detected across various tissues but especially noted in the brain and liver suggesting a role in specific physiological processes. It does not have an extensively documented function yet some studies indicate involvement in cellular structures and processes.

Biological function summary

The protein C14orf133 participates in maintaining cellular functions through association with certain complexes. It might be involved in cellular organization or structural maintenance. Although detailed mechanisms are still emerging its expression pattern suggests an influence in central processes related to cellular architecture or signal transduction within these tissues possibly interconnected with broader cellular systems.

Pathways

The protein C14orf133 often influences or interacts with well-defined cellular pathways. It might engage in cellular signaling pathways or have a role linked to actin cytoskeleton organization. Related proteins in these pathways might include actin-binding proteins or signaling mediators that guide cellular responses. Understanding its exact role in these pathways can highlight its impact on cellular behavior and physiological function.

Research suggests C14orf133 may relate to conditions affecting the nervous system or liver. Connections to neurodegenerative diseases or hepatic dysfunctions have been proposed. Proteins interacting with C14orf133 in these contexts may include those involved in neurogenic signaling or metabolic regulation within the liver. Investigating these connections further may uncover potential therapeutic insights or diagnostic markers related to these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Proposed to be involved in endosomal maturation implicating in part VPS33B. In epithelial cells, the VPS33B : VIPAS39 complex may play a role in the apical RAB11A-dependent recycling pathway and in the maintenance of the apical-basolateral polarity (PubMed : 20190753). May play a role in lysosomal trafficking, probably via association with the core HOPS complex in a discrete population of endosomes; the functions seems to be independent of VPS33B (PubMed : 19109425). May play a role in vesicular trafficking during spermatogenesis (By similarity). May be involved in direct or indirect transcriptional regulation of E-cadherin (By similarity).

See full target information VIPAS39

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:8334 PubMed39333072

2024

USF2 and TFEB compete in regulating lysosomal and autophagy genes.

Applications

Unspecified application

Species

Unspecified reactive species

Jaebeom Kim,Young Suk Yu,Yehwa Choi,Do Hui Lee,Soobin Han,Junhee Kwon,Taichi Noda,Masahito Ikawa,Dongha Kim,Hyunkyung Kim,Andrea Ballabio,Keun Il Kim,Sung Hee Baek

The Journal of biological chemistry 299:104718 PubMed37062417

2023

The Sec1-Munc18 protein VPS33B forms a uniquely bidirectional complex with VPS16B.

Applications

Unspecified application

Species

Unspecified reactive species

Richard J Y Liu,Yusef Al-Molieh,Shao Z Chen,Marko Drobac,Denisa Urban,Chang H Chen,Helen H Y Yao,Ryan S Q Geng,Ling Li,Fred G Pluthero,Samir Benlekbir,John L Rubinstein,Walter H A Kahr

American journal of translational research 10:16-39 PubMed29422991

2018

Preeclampsia is associated with hypermethylation of IGF-1 promoter mediated by DNMT1.

Applications

Unspecified application

Species

Unspecified reactive species

Min Ma,Qiong-Jie Zhou,Yu Xiong,Bin Li,Xiao-Tian Li

The Journal of pathology 240:108-19 PubMed27319744

2016

Vps33b regulates Vwf-positive vesicular trafficking in megakaryocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Dai,Yeling Lu,Conghui Wang,Xue Chen,Xuemei Fan,Hao Gu,Xiaolin Wu,Kemin Wang,T Kent Gartner,Junke Zheng,Guoqiang Chen,Xuefeng Wang,Junling Liu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-C14orf133 antibody

Immunoprecipitation - Anti-C14orf133 antibody (AB125084)

Western blot - Anti-C14orf133 antibody (AB125084)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise