Rabbit Polyclonal C14orf133 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human VIPAS39 aa 400 to C-terminus.
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Publications
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- The Journal of biological chemistry 299:1047182023The Sec1-Munc18 protein VPS33B forms a uniquely bidirectional complex with VPS16B.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRichard J Y Liu,Yusef Al-Molieh,Shao Z Chen,Marko Drobac,Denisa Urban,Chang H Chen,Helen H Y Yao,Ryan S Q Geng,Ling Li,Fred G Pluthero,Samir Benlekbir,John L Rubinstein,Walter H A KahrPubMed 37062417
- The Journal of pathology 240:108-192016Vps33b regulates Vwf-positive vesicular trafficking in megakaryocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJing Dai,Yeling Lu,Conghui Wang,Xue Chen,Xuemei Fan,Hao Gu,Xiaolin Wu,Kemin Wang,T Kent Gartner,Junke Zheng,Guoqiang Chen,Xuefeng Wang,Junling LiuPubMed 27319744
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris citrate/phosphate- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Synthetic Peptide within Human VIPAS39 aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9H9C1
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Expected | Tested |
| Chimpanzee | Predicted | Predicted |
| Gorilla | Predicted | Predicted |
| Orangutan | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-10.00000 µg/mg of lysate | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee, Rhesus monkey, Gorilla, Orangutan | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee, Rhesus monkey, Gorilla, Orangutan | Dilution info - | Notes - |
Associated Products
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Target data
Function
Proposed to be involved in endosomal maturation implicating in part VPS33B. In epithelial cells, the VPS33B:VIPAS39 complex may play a role in the apical RAB11A-dependent recycling pathway and in the maintenance of the apical-basolateral polarity (PubMed:20190753). May play a role in lysosomal trafficking, probably via association with the core HOPS complex in a discrete population of endosomes; the functions seems to be independent of VPS33B (PubMed:19109425). May play a role in vesicular trafficking during spermatogenesis (By similarity). May be involved in direct or indirect transcriptional regulation of E-cadherin (By similarity).
Alternative names
Spermatogenesis-defective protein 39 homolog, hSPE-39, VPS33B-interacting protein in apical-basolateral polarity regulator, VPS33B-interacting protein in polarity and apical restriction, VIPAR, SPE39, C14orf133, VIPAS39
Recommended products
Rabbit Polyclonal C14orf133 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human VIPAS39 aa 400 to C-terminus.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris citrate/phosphate- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Synthetic Peptide within Human VIPAS39 aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9H9C1
- Purification technique
Affinity purification Immunogen
- Concentration
- Purification notes
ab125084 was affinity purified using an epitope specific to C14orf133 immobilized on solid support.
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The C14orf133 protein also known as chromosome 14 open reading frame 133 is an intriguing target in biological research. This protein has a molecular mass of approximately 45 kDa. C14orf133 expression is detected across various tissues but especially noted in the brain and liver suggesting a role in specific physiological processes. It does not have an extensively documented function yet some studies indicate involvement in cellular structures and processes.
- Biological function summary
The protein C14orf133 participates in maintaining cellular functions through association with certain complexes. It might be involved in cellular organization or structural maintenance. Although detailed mechanisms are still emerging its expression pattern suggests an influence in central processes related to cellular architecture or signal transduction within these tissues possibly interconnected with broader cellular systems.
- Pathways
The protein C14orf133 often influences or interacts with well-defined cellular pathways. It might engage in cellular signaling pathways or have a role linked to actin cytoskeleton organization. Related proteins in these pathways might include actin-binding proteins or signaling mediators that guide cellular responses. Understanding its exact role in these pathways can highlight its impact on cellular behavior and physiological function.
- Associated diseases and disorders
Research suggests C14orf133 may relate to conditions affecting the nervous system or liver. Connections to neurodegenerative diseases or hepatic dysfunctions have been proposed. Proteins interacting with C14orf133 in these contexts may include those involved in neurogenic signaling or metabolic regulation within the liver. Investigating these connections further may uncover potential therapeutic insights or diagnostic markers related to these diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunoprecipitation - Anti-C14orf133 antibody (ab125084)
Detection of C14orf133 by Western Blot of Immunprecipitate.
ab125084 at 1µg/ml staining C14orf133 in HeLa whole cell lysate immunoprecipitated using ab125084 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 30 seconds.All lanes: Immunoprecipitation - Anti-C14orf133 antibody (ab125084)
Predicted band size: 57 kDa
Western blot - Anti-C14orf133 antibody (ab125084)
All lanes: Western blot - Anti-C14orf133 antibody (ab125084) at 0.1 µg/mL
Lane 1: HeLa whole cell lysate at 50 µg
Lane 2: HeLa whole cell lysate at 15 µg
Lane 3: 293T whole cell lysate at 50 µg
Lane 4: Jurkat whole cell lysate at 50 µg
Lane 5: NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 57 kDa
Exposure time: 30s
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