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AB250954

Anti-C14orf166 antibody [EPR15799] - BSA and Azide free

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Rabbit Recombinant Monoclonal C14orf166 antibody. Carrier free. Suitable for ICC/IF, IP, WB, IHC-P and reacts with Human, Rat, Mouse samples.

View Alternative Names

C14orf166, CGI-99, RTRAF, CLE7 homolog, CLE, hCLE

6 Images
Western blot - Anti-C14orf166 antibody [EPR15799] - BSA and Azide free (AB250954)
  • WB

Supplier Data

Western blot - Anti-C14orf166 antibody [EPR15799] - BSA and Azide free (AB250954)

This data was developed using ab188326, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-C14orf166 antibody [EPR15799] (<a href='/en-us/products/primary-antibodies/c14orf166-antibody-epr15799-ab188326'>ab188326</a>) at 1/1000 dilution

Lane 1:

C6 cell lysate at 10 µg

Lane 2:

Raw264.7 cell lysate at 10 µg

Lane 3:

PC12 cell lysate at 10 µg

Lane 4:

NIH3T3 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 28 kDa

false

Immunocytochemistry/ Immunofluorescence - Anti-C14orf166 antibody [EPR15799] - BSA and Azide free (AB250954)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-C14orf166 antibody [EPR15799] - BSA and Azide free (AB250954)

This data was developed using ab188326, the same antibody clone in a different buffer formulation.

Immunofluorescence analysis of U87-MG cells labeling C14orf166 using ab188326 at 1/100 dilution. A goat anti rabbit IgG (Alexa Fluor488) at 1/200 dilution was used as secondary antibody. Cells were fixed with 4% paraformaldehyde. Counterstain : DAPI.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C14orf166 antibody [EPR15799] - BSA and Azide free (AB250954)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C14orf166 antibody [EPR15799] - BSA and Azide free (AB250954)

This data was developed using ab188326, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human astrocytoma tissue labeling C14orf166 using ab188326 at 1/100 dilution. A ready to use HRP polymer for rabbit IgG (prediluted) was used as secondary. Counterstain : Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C14orf166 antibody [EPR15799] - BSA and Azide free (AB250954)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C14orf166 antibody [EPR15799] - BSA and Azide free (AB250954)

This data was developed using ab188326, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human fetal kidney tissue labeling C14orf166 using ab188326 at 1/100 dilution. A ready to use HRP polymer for rabbit IgG (prediluted) was used as secondary. Counterstain : Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunoprecipitation - Anti-C14orf166 antibody [EPR15799] - BSA and Azide free (AB250954)
  • IP

Supplier Data

Immunoprecipitation - Anti-C14orf166 antibody [EPR15799] - BSA and Azide free (AB250954)

This data was developed using ab188326, the same antibody clone in a different buffer formulation.

Immunoprecipitation analysis of 293T cell lysate labeling C14orf166 using ab188326 at 1/50 dilution (Lane 1). An anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody. Lane 2 : PBS instead of 293T cell lysate.

All lanes:

Immunoprecipitation - Anti-C14orf166 antibody [EPR15799] (<a href='/en-us/products/primary-antibodies/c14orf166-antibody-epr15799-ab188326'>ab188326</a>)

Predicted band size: 28 kDa

false

Western blot - Anti-C14orf166 antibody [EPR15799] - BSA and Azide free (AB250954)
  • WB

Supplier Data

Western blot - Anti-C14orf166 antibody [EPR15799] - BSA and Azide free (AB250954)

This data was developed using ab188326, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-C14orf166 antibody [EPR15799] (<a href='/en-us/products/primary-antibodies/c14orf166-antibody-epr15799-ab188326'>ab188326</a>) at 1/10000 dilution

Lane 1:

Ramos cell lysate at 20 µg

Lane 2:

293T cell lysate at 20 µg

Lane 3:

Jurkat cell lysate at 20 µg

Lane 4:

U87-MG cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 28 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15799

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

IP, ICC/IF, WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

C14orf166 also known as TIPH2 is a protein encoded by the C14orf166 gene. This protein has a molecular mass of approximately 38 kDa. It plays a role in RNA metabolism particularly in the export of messenger RNA (mRNA) from the nucleus to the cytoplasm. Expression of C14orf166 occurs in various tissues with higher levels often found in actively dividing and metabolically active cells.
Biological function summary

C14orf166 integrates into the transcriptional regulation process by forming part of a complex involved in RNA processing. It interacts with components of the transcription-export (TREX) complex which is essential for coupling mRNA processing with export. This integration allows C14orf166 to assist in maintaining efficient mRNA transport and translation important for normal cellular function and stress response.

Pathways

C14orf166 significantly influences gene expression regulation and participates in the mRNA processing pathways. It interacts with proteins such as Aly/REF and UAP56 within the TREX complex importantly impacting the efficiency of mRNA export. By securing efficient mRNA transport C14orf166 supports critical biological pathways like the unfolded protein response and cellular proliferation.

Aberrant expression or function of C14orf166 has connections to cancer particularly breast cancer. Dysregulation in its function can lead to improper mRNA export contributing to tumorigenesis. Furthermore C14orf166 shows relations to viral infections; for example it intersects with pathways involving Nucleophosmin (NPM1) which are exploited by viruses such as influenza. This makes C14orf166 a potential target for therapeutic strategies aiming to manage these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

RNA-binding protein involved in modulation of mRNA transcription by Polymerase II (PubMed : 16950395). Component of the tRNA-splicing ligase complex and is required for tRNA ligation (PubMed : 24870230). May be required for RNA transport (PubMed : 24608264).. (Microbial infection) In case of infection by influenza virus A (IVA), is involved in viral replication (PubMed : 21900157).
See full target information RTRAF
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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