AB192861

Anti-C14orf169 / NO66 antibody [EPR18656]

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal C14orf169 / NO66 antibody. Suitable for IP, WB and reacts with Human, Mouse, Rat samples.

View Alternative Names

C14orf169, MAPJD, NO66, RIOX1, Ribosomal oxygenase 1, 60S ribosomal protein L8 histidine hydroxylase, Bifunctional lysine-specific demethylase and histidyl-hydroxylase NO66, Myc-associated protein with JmjC domain, Nucleolar protein 66, Ribosomal oxygenase NO66, hsNO66, ROX

4 Images

Supplier Data

Western blot - Anti-C14orf169 / NO66 antibody [EPR18656] (AB192861)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-C14orf169 / NO66 antibody [EPR18656] (ab192861) at 1/1000 dilution

Lane 1:

C6 (Rat glial tumor cell line) whole cell lysate at 10 µg

Lane 2:

RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

Lane 3:

PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate at 10 µg

Lane 4:

NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 71 kDa

Observed band size: 71 kDa

false

Exposure time: 3min

Supplier Data

Immunoprecipitation - Anti-C14orf169 / NO66 antibody [EPR18656] (AB192861)

C14orf169 / NO66 was immunoprecipitated from 1mg of HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate with ab192861 at 1/50 dilution.

Western blot was performed from the immunoprecipitate using ab192861 at 1/1000 dilution.

VeriBlot for IP Detection Reagent(HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : HEK-293 whole cell lysate, 10μg (Input).

Lane 2 : ab192861 IP in HEK-293 whole cell lysate.

Lane 3 : Rabbit IgG,monoclonal [EPR18656]-Isotype Control (ab172730) instead of ab192861 in HEK-293 whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 minutes.

All lanes:

Immunoprecipitation - Anti-C14orf169 / NO66 antibody [EPR18656] (ab192861)

Predicted band size: 71 kDa

false

Supplier Data

Western blot - Anti-C14orf169 / NO66 antibody [EPR18656] (AB192861)

Blocking/Dilution buffer : 5% NFDM/TBST.

The predicted MW of human's, mouse's and rat's NO66 are 71kDa, 68kDa and 67kDa, respectively.

All lanes:

Western blot - Anti-C14orf169 / NO66 antibody [EPR18656] (ab192861) at 1/1000 dilution

Lane 1:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 2:

HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg

Lane 3:

U-2 OS (Human bone osteosarcoma epithelial cell line) whole cell lysate at 20 µg

Lane 4:

NCCIT (Human pluripotent embryonic carcinoma cell line) whole cell lysate at 20 µg

Lane 5:

F9 (Mouse embryonic testicular cancer cell line) whole cell lysate at 20 µg

Lane 6:

LLC (Mouse lung carcinoma cell line) whole cell lysate at 20 µg

Lane 7:

mESC (Mouse embryonic stem cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 71 kDa

Observed band size: 71 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-C14orf169 / NO66 antibody [EPR18656] (AB192861)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-C14orf169 / NO66 antibody [EPR18656] (ab192861) at 1/200 dilution

Lane 1:

Human fetal liver lysate at 10 µg

Lane 2:

Mouse lung lysate at 10 µg

Secondary

Lane 1:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Lane 2:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 71 kDa

Observed band size: 71 kDa

false

Exposure time: 3min

Carrier free

Anti-C14orf169 / NO66 antibody [EPR18656] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR18656

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/50", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

C14orf169 also known as NO66 is a nuclear protein implicated in chromatin regulation and protein synthesis. It has an estimated molecular mass of approximately 85 kDa. This protein shows widespread expression in various tissues including high levels in the brain and liver. C14orf169 possesses JmjC domain which provides it with demethylase activity influencing histone modifications that are critical for chromatin structure and gene expression regulation.

Biological function summary

C14orf169 functions as a part of nuclear complexes involved in transcriptional regulation. It interacts with other chromatin-associated factors ensuring proper histone modification which is necessary for modulating gene expression. This protein's role in regulating chromatin's epigenetic state connects it to important cellular processes such as cell cycle progression and response to stress.

Pathways

C14orf169 integrates within histone modification and transcriptional regulation pathways. Its demethylase activity influences the H3K4me3 and H3K36me3 marks important for transcriptional activation or repression. C14orf169 shows interactions with other proteins such as histone demethylases and methyltransferases that cooperate in modifying chromatin structure affecting gene transcription patterns.

C14orf169's dysregulation associates with cancer types including prostate cancer. Changes in its expression may lead to altered chromatin states affecting oncogene and tumor suppressor gene regulation. Additionally C14orf169 connects with ubiquitin-proteasome pathways implicating it in neurodegenerative disorders where protein degradation becomes impaired specifically in conditions like Alzheimer's disease.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Oxygenase that can act as both a histone lysine demethylase and a ribosomal histidine hydroxylase (PubMed : 23103944). Specifically demethylates 'Lys-4' (H3K4me) and 'Lys-36' (H3K36me) of histone H3, thereby playing a central role in histone code (By similarity). Preferentially demethylates trimethylated H3 'Lys-4' (H3K4me3) and monomethylated H3 'Lys-4' (H3K4me1) residues, while it has weaker activity for dimethylated H3 'Lys-36' (H3K36me2) (By similarity). Acts as a regulator of osteoblast differentiation via its interaction with SP7/OSX by demethylating H3K4me and H3K36me, thereby inhibiting SP7/OSX-mediated promoter activation (By similarity). Also catalyzes demethylation of non-histone proteins, such as CGAS : demethylation of monomethylated CGAS promotes interaction between CGAS and PARP1, followed by PARP1 inactivation (By similarity). Also catalyzes the hydroxylation of 60S ribosomal protein L8 on 'His-216', thereby playing a role in ribosome biogenesis (PubMed : 23103944). Participates in MYC-induced transcriptional activation (PubMed : 17308053).

See full target information RIOX1

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-C14orf169 / NO66 antibody [EPR18656]

Western blot - Anti-C14orf169 / NO66 antibody [EPR18656] (AB192861)

Immunoprecipitation - Anti-C14orf169 / NO66 antibody [EPR18656] (AB192861)

Western blot - Anti-C14orf169 / NO66 antibody [EPR18656] (AB192861)

Western blot - Anti-C14orf169 / NO66 antibody [EPR18656] (AB192861)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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Product promise