Anti-C14orf169 / NO66 antibody [EPR18754] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C14orf169 / NO66 antibody [EPR18754] - BSA and Azide free (AB251184)

This data was developed using ab194292, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue labeling C14orf169 / NO66 with ab194292 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on epithelial cells of human colon. Counter-stained with Hematoxylin.
Secondary antibody only control : PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IP

Supplier Data

Immunoprecipitation - Anti-C14orf169 / NO66 antibody [EPR18754] - BSA and Azide free (AB251184)

This data was developed using ab194292, the same antibody clone in a different buffer formulation.

Immunoprecipitation of C14orf169 / NO66 from 1mg of HEK293 (Human epithelial cells from embryonic kidney) whole cell lysate with ab194292 at 1/1000 dilution. Western blot was performed from the immunoprecipitate using ab194292 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/10000 was used for detection.
Lane 1 : HEK293 whole cell lysate 10μg (Input).
Lane 2 : ab194292 IP in HEK293 whole cell lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab194292 in HEK293 whole cell lysates.
Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 10 second.

All lanes:

Immunoprecipitation - Anti-C14orf169 / NO66 antibody [EPR18754] (<a href='/en-us/products/primary-antibodies/c14orf169-no66-antibody-epr18754-ab194292'>ab194292</a>)

Predicted band size: 71 kDa

false

• WB

Supplier Data

Western blot - Anti-C14orf169 / NO66 antibody [EPR18754] - BSA and Azide free (AB251184)

This data was developed using ab194292, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Lanes 1 - 4:

Western blot - Anti-C14orf169 / NO66 antibody [EPR18754] (<a href='/en-us/products/primary-antibodies/c14orf169-no66-antibody-epr18754-ab194292'>ab194292</a>) at 1/1000 dilution

Lane 5:

Western blot - Anti-C14orf169 / NO66 antibody [EPR18754] (<a href='/en-us/products/primary-antibodies/c14orf169-no66-antibody-epr18754-ab194292'>ab194292</a>) at 1/5000 dilution

Lane 1:

HEK293 (Human epithelial cells from embryonic kidney) whole cell lysate at 10 µg

Lane 2:

U-2 OS (Human bone osteosarcoma epithelial cell line) whole cell lysate at 10 µg

Lane 3:

A549 (Human lung carcinoma) whole cell lysate at 10 µg

Lane 4:

NCCIT (human pluripotent embryonic carcinoma) whole cell lysate at 10 µg

Lane 5:

MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 71 kDa

false

Exposure time: 3min

• WB

Supplier Data

Western blot - Anti-C14orf169 / NO66 antibody [EPR18754] - BSA and Azide free (AB251184)

This data was developed using ab194292, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-C14orf169 / NO66 antibody [EPR18754] (<a href='/en-us/products/primary-antibodies/c14orf169-no66-antibody-epr18754-ab194292'>ab194292</a>) at 1/1000 dilution

Lane 1:

Human fetal brain tissue lysate at 10 µg

Lane 2:

Human fetal kidney tissue lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 71 kDa

false

Exposure time: 3min