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AB250621

Anti-C15orf24 antibody [EPR14097] - BSA and Azide free

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Rabbit Recombinant Monoclonal C15orf24 antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

C11orf3, C15orf24, HT022, UNQ905/PRO1926, EMC7, Endoplasmic reticulum membrane protein complex subunit 7, ER membrane protein complex subunit 7

1 Images
Western blot - Anti-C15orf24 antibody [EPR14097] - BSA and Azide free (AB250621)
  • WB

Supplier Data

Western blot - Anti-C15orf24 antibody [EPR14097] - BSA and Azide free (AB250621)

This data was developed using ab182568, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-C15orf24 antibody [EPR14097] (<a href='/en-us/products/primary-antibodies/c15orf24-antibody-epr14097-ab182568'>ab182568</a>) at 1/10000 dilution

Lane 1:

Human fetal brain tissue lysate at 20 µg

Lane 2:

HeLa cell line lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

Predicted band size: 26 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14097

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Anti-HMGB1 antibody

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Primary Antibodies

AB92516

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Primary Antibodies

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Anti-CRTC3 antibody [EPR3440]

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  • 5
  • 2
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Reactivity data

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Product details

ab250621 is the carrier-free version of ab182568.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

C15orf24 also known as Chromosome 15 Open Reading Frame 24 is a lesser characterized protein that plays a role in cellular processes. The protein has an approximate mass of 45 kDa. Expression studies show C15orf24 expression in several tissues including heart and liver hinting at its potential roles in important physiological processes. Researchers still work to uncover the full scope of its mechanical function.
Biological function summary

The activities of C15orf24 suggest an involvement in regulating cellular homeostasis. While it has not been fully integrated into any specific protein complex initial findings propose that C15orf24 may have an interaction with proteins involved in cellular signaling. Its expression pattern and interactions imply it may modulate cellular responses to specific stimuli but further studies are needed.

Pathways

C15orf24 has been connected to cellular stress response mechanisms. Proteins related to C15orf24 through these pathways include MAPK signaling components which are integral in stress response and apoptosis pathways. Research indicates that C15orf24 might influence these pathways impacting how cells react to internal and external stress factors.

Findings have linked C15orf24 with some aspects of cancer biology and neurodegenerative conditions. Studies suggest that aberrations in C15orf24 expression might relate to the progression of specific cancers potentially through its interaction with proteins such as p53 which regulates the cell cycle. Its potential involvement in neurodegenerative disorders also warrants further investigation as it might affect amyloid-beta metabolism tying it to Alzheimer’s disease research.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Part of the endoplasmic reticulum membrane protein complex (EMC) that enables the energy-independent insertion into endoplasmic reticulum membranes of newly synthesized membrane proteins (PubMed : 29242231, PubMed : 29809151, PubMed : 30415835, PubMed : 32439656, PubMed : 32459176). Preferentially accommodates proteins with transmembrane domains that are weakly hydrophobic or contain destabilizing features such as charged and aromatic residues (PubMed : 29242231, PubMed : 29809151, PubMed : 30415835). Involved in the cotranslational insertion of multi-pass membrane proteins in which stop-transfer membrane-anchor sequences become ER membrane spanning helices (PubMed : 29809151, PubMed : 30415835). It is also required for the post-translational insertion of tail-anchored/TA proteins in endoplasmic reticulum membranes (PubMed : 29242231, PubMed : 29809151). By mediating the proper cotranslational insertion of N-terminal transmembrane domains in an N-exo topology, with translocated N-terminus in the lumen of the ER, controls the topology of multi-pass membrane proteins like the G protein-coupled receptors (PubMed : 30415835). By regulating the insertion of various proteins in membranes, it is indirectly involved in many cellular processes (Probable).
See full target information EMC7
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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