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AB255269

Anti-C15orf41 antibody [EPR21169-28] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal C15orf41 antibody. Carrier free. Suitable for IP, WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

C15orf41, CDIN1, CDAN1-interacting nuclease 1, Protein HH114

1 Images
Immunoprecipitation - Anti-C15orf41 antibody [EPR21169-28] - BSA and Azide free (AB255269)
  • IP

Unknown

Immunoprecipitation - Anti-C15orf41 antibody [EPR21169-28] - BSA and Azide free (AB255269)

C15orf41 was immunoprecipitated from 0.35 mg HEK-293 (human embryonic kidney epithelial cell) whole cell lysate with ab215190 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab215190 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used at 1/5000 dilution.

Lane 1 : HEK-293 whole cell lysate 10 μg (Input).
Lane 2 : ab215190 IP in HEK-293 whole cell lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab215190 in HEK-293 whole cell lysate.

Blocking/Dilution buffer : 5% NFDM/TBST.
Exposure time : 3 minutes.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab215190).

All lanes:

Immunoprecipitation - Anti-C15orf41 antibody [EPR21169-28] (<a href='/en-us/products/primary-antibodies/c15orf41-antibody-epr21169-28-ab215190'>ab215190</a>)

Predicted band size: 32 kDa

Observed band size: 32 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR21169-28

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Product details

ab255269 is the carrier-free version of ab215190.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

C15orf41 also known as chromosome 15 open reading frame 41 is a protein with a molecular mass of approximately 38 kDa. This protein is expressed widely in various tissues showing higher expression levels in the liver heart and skeletal muscle. C15orf41 is characterized by its ability to bind nucleic acids suggesting a role in nucleic acid metabolism. The gene encoding C15orf41 is located on chromosome 15 reflecting its name.
Biological function summary

The functions of C15orf41 are closely related to its involvement in DNA replication and repair processes. As part of a larger protein complex C15orf41 plays a role in maintaining genome stability. It assists in the resolution of DNA structures that could otherwise pose challenges during replication. The protein interacts with other DNA repair machinery components ensuring effective genomic maintenance.

Pathways

C15orf41 participates in key biological processes tied to DNA repair and replication stress response pathways. This protein interfaces significantly with the homologous recombination repair pathway working alongside proteins such as BRCA2. Additionally C15orf41 influences the ATRIP-ATR axis which serves to stabilize the replication fork and DNA damage checkpoint activation ensuring proper DNA replication completion.

Alterations in C15orf41 expression or function can contribute to genomic instability-associated conditions. Studies associate this protein with cancer where its dysregulation may result in unchecked cell proliferation owing to ineffective DNA repair. Furthermore C15orf41 has connections to Fanconi anemia pathway components where defects can lead to hematological abnormalities and predisposition to malignancies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in erythroid cell differentiation.
See full target information CDIN1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in microbiology 13:861734 PubMed35359726

2022

Vitamin D3 Suppresses Human Cytomegalovirus-Induced Vascular Endothelial Apoptosis Rectification of Paradoxical m6A Modification of Mitochondrial Calcium Uniporter mRNA, Which Is Regulated by METTL3 and YTHDF3.

Applications

Unspecified application

Species

Unspecified reactive species

Wenbo Zhu,Hongbo Zhang,Shao Wang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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