AB224561

Anti-C16orf48 antibody

Name: Anti-C16orf48 antibody (ab224561) | Abcam
Brand: Abcam Limited
SKU: AB224561

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(2 Publications)

Rabbit Polyclonal C16orf48 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ENKD1 aa 200-300.

View Alternative Names

C16orf48, UNQ6410/PRO21183, ENKD1, Enkurin domain-containing protein 1

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-C16orf48 antibody (AB224561)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-C16orf48 antibody (AB224561)

PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for C16orf48 (green) using ab224561 at 4 μg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C16orf48 antibody (AB224561)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C16orf48 antibody (AB224561)

Paraffin-embedded human testis tissue stained for C16orf48 using ab224561 at 1/200 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-C16orf48 antibody (AB224561)

All lanes:

Western blot - Anti-C16orf48 antibody (ab224561) at 1/100 dilution

Lane 1:

Vector only transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate

Lane 2:

C16orf48 overexpression HEK-293T cell lysate (Co-expressed with a C-terminal myc-DDK tag, ~3.1 kDa)

Predicted band size: 38 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human ENKD1 aa 200-300. The exact immunogen used to generate this antibody is proprietary information.

Q9H0I2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

C16orf48 also known as FLJ20489 is a protein with an approximate molecular mass of 13 kDa. It exhibits higher expression levels in certain tissues including the brain and liver indicating a tissue-specific role. The protein's mechanical function involves RNA binding suggesting its involvement in RNA processing or regulation. The well-defined binding domain aids in precise targeting within the cellular environment reinforcing its regulatory capacities.

Biological function summary

The actions of C16orf48 influence cellular processes particularly in gene expression regulation. C16orf48 participates in a multi-protein complex suggesting its interactions with other macromolecules elevate its functional significance. While not all the protein interactions and functions are thoroughly characterized its ability to bind RNA points towards an important role in post-transcriptional modifications or other RNA-related activities affecting how genes are expressed or silenced in different conditions.

Pathways

C16orf48 is implicated in pathways linked to RNA metabolic processes and gene expression regulation. Evidence shows involvement in the mRNA splicing pathway where it potentially interacts with well-studied splicing factors. The presence of related proteins like U2AF2 or SF3B1 within these pathways suggests that C16orf48 might influence alternative splicing events which can change the protein output from genes and lead to variations in cellular functions.

Mutations or dysregulation of C16orf48 have been associated with neurological disorders and liver diseases. In particular aberrant expression of C16orf48 can have implications in conditions such as Alzheimer's disease where connection to proteins like tau or amyloid precursor protein (APP) suggests a regulatory role in their gene expression. Additionally liver disease connections have drawn attention towards C16orf48's potential impact in hepatocyte function making it an important target for further research in understanding complex disease mechanisms.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Microtubule-binding protein which regulates microtubule organization and stability (PubMed : 35072334, PubMed : 35197565). Promotes the stability of astral microtubules and facilitates the proper orientation of the mitotic spindle (PubMed : 35197565). This allows the oriented division of basal keratinocytes and contributes to epidermal stratification (By similarity). Required for the assembly of both primary and motile cilia (PubMed : 35301795). Destabilizes the interaction between CCP110 and CEP97 by competing with CEP97 for binding to CCP110 which promotes the removal of CCP110 and CEP97 from the mother centriole and allows the initiation of ciliogenesis (PubMed : 35301795).

See full target information ENKD1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2404067 PubMed39373352

2024

CYLD Maintains Retinal Homeostasis by Deubiquitinating ENKD1 and Promoting the Phagocytosis of Photoreceptor Outer Segments.

Applications

Unspecified application

Species

Unspecified reactive species

Song Yang,Fan Yu,Mulin Yang,Hua Ni,Weiwen Bu,Hanxiao Yin,Jia Yang,Weishu Wang,Denghui Zhai,Xuemei Wu,Nan Ma,Te Li,Huijie Hao,Jie Ran,Ting Song,Dengwen Li,Sei Yoshida,Quanlong Lu,Yunfan Yang,Jun Zhou,Min Liu

Journal of cellular physiology 238:1308-1323 PubMed36960713

2023

Upregulation of ENKD1 disrupts cellular homeostasis to promote lymphoma development.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Song,Na He,Ziqian Hao,Yunfan Yang

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-C16orf48 antibody

Immunocytochemistry/ Immunofluorescence - Anti-C16orf48 antibody (AB224561)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C16orf48 antibody (AB224561)

Western blot - Anti-C16orf48 antibody (AB224561)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise