AB181987

Anti-C16orf70 antibody [EPR14035]

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(2 Publications)

Rabbit Recombinant Monoclonal C16orf70 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications.

View Alternative Names

C16orf6, C16orf70, PHAF1, Phagosome assembly factor 1

2 Images

Supplier Data

Immunoprecipitation - Anti-C16orf70 antibody [EPR14035] (AB181987)

Immunoprecipitation of SH-SY5Y cell lysate, labeling C16orf70 usinf ab181987 at a 1/30 dilution (lane 1) or a negative control (lane 2). Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at a 1/5000 dilution.

All lanes:

Immunoprecipitation - Anti-C16orf70 antibody [EPR14035] (ab181987)

Predicted band size: 48 kDa

false

Supplier Data

Western blot - Anti-C16orf70 antibody [EPR14035] (AB181987)

All lanes:

Western blot - Anti-C16orf70 antibody [EPR14035] (ab181987) at 1/1000 dilution

Lane 1:

Human fetal brain cell lysate at 20 µg

Lane 2:

U87-MG cell lysate at 20 µg

Lane 3:

SH-SY5Y cell lysate at 20 µg

Lane 4:

JAR cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 48 kDa

false

Carrier free

Anti-C16orf70 antibody [EPR14035] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14035

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

C16orf70 also known as Chromosome 16 Open Reading Frame 70 codes for a protein with a mass approximately of 17 kDa. This protein is mainly expressed in tissues such as the liver and lungs. C16orf70 may involve various functions in cellular processes but it lacks extensive characterization. Researchers aim to understand its role in different cellular contexts as it may hold significance in tissue-specific functions.

Biological function summary

The actions of C16orf70 suggest involvement in cellular signaling pathways. Studies hint at its participation in protein complexes although exact partners require further identification. Its presence in certain tissues implies a potential role linked to intercellular communication possibly influencing cell growth or immune responses. Further research continues to explore these biological interactions.

Pathways

C16orf70 might engage in critical roles within pathways like the Wnt signaling and cellular senescence. Its interactions with other proteins possibly including those from the Wnt pathway suggest involvement in regulating gene expression and maintaining cellular health. Understanding its precise function in these pathways will provide deeper insights into cell regulation and development.

Researchers link C16orf70 to conditions like cancer and chronic obstructive pulmonary disease (COPD). The protein's altered expression or function may interfere with cellular pathways highlighting associations with oncogenic or inflammatory processes. Studies investigate its connection with proteins involved in these disorders aiming to uncover potential therapeutic targets or biomarkers.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Plays a regulatory role in autophagic activity. In complex with BCAS3, associates with the autophagosome formation site during both non-selective and selective autophagy.

See full target information PHAF1

style

left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Molecular carcinogenesis 64:652-667 PubMed39783768

2025

RNF7-Mediated ROS Targets Malignant Phenotype and Radiotherapy Sensitivity in Glioma With Different IDH1 Genotypes.

Applications

Unspecified application

Species

Unspecified reactive species

Yiran Tao,Zimin Shi,Xianyin Liang,Yuqian Zheng,Lirui Dai,Xiang Li,Zian Li,Wulong Liang,Gaojie Bai,Hao Li,Yuan Lyu,Junqi Li,Tao Zhang,Weihua Hu,Shaolong Zhou,Qiao Shan,Xudong Fu,Xinjun Wang

The Journal of clinical investigation 134: PubMed38869949

2024

C16ORF70/MYTHO promotes healthy aging in C.elegans and prevents cellular senescence in mammals.

Applications

Unspecified application

Species

Unspecified reactive species

Anais Franco-Romero,Valeria Morbidoni,Giulia Milan,Roberta Sartori,Jesper Wulff,Vanina Romanello,Andrea Armani,Leonardo Salviati,Maria Conte,Stefano Salvioli,Claudio Franceschi,Viviana Buonomo,Casey O Swoboda,Paolo Grumati,Luca Pannone,Simone Martinelli,Harold Bj Jefferies,Ivan Dikic,Jennifer van der Laan,Filipe Cabreiro,Douglas P Millay,Sharon A Tooze,Eva Trevisson,Marco Sandri

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-C16orf70 antibody [EPR14035]

Immunoprecipitation - Anti-C16orf70 antibody [EPR14035] (AB181987)

Western blot - Anti-C16orf70 antibody [EPR14035] (AB181987)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise