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Rabbit Recombinant Monoclonal C16orf70 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples.

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Images

Immunoprecipitation - Anti-C16orf70 antibody [EPR14035] - BSA and Azide free (AB250554), expandable thumbnail
  • Western blot - Anti-C16orf70 antibody [EPR14035] - BSA and Azide free (AB250554), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWB
Human
Tested
Tested
Mouse
Predicted
Predicted
Rat
Predicted
Predicted

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Plays a regulatory role in autophagic activity. In complex with BCAS3, associates with the autophagosome formation site during both non-selective and selective autophagy.

Alternative names

Recommended products

Rabbit Recombinant Monoclonal C16orf70 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR14035
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab250554 is the carrier-free version of Anti-C16orf70 antibody [EPR14035] ab181987.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

C16orf70 also known as Chromosome 16 Open Reading Frame 70 codes for a protein with a mass approximately of 17 kDa. This protein is mainly expressed in tissues such as the liver and lungs. C16orf70 may involve various functions in cellular processes but it lacks extensive characterization. Researchers aim to understand its role in different cellular contexts as it may hold significance in tissue-specific functions.

Biological function summary

The actions of C16orf70 suggest involvement in cellular signaling pathways. Studies hint at its participation in protein complexes although exact partners require further identification. Its presence in certain tissues implies a potential role linked to intercellular communication possibly influencing cell growth or immune responses. Further research continues to explore these biological interactions.

Pathways

C16orf70 might engage in critical roles within pathways like the Wnt signaling and cellular senescence. Its interactions with other proteins possibly including those from the Wnt pathway suggest involvement in regulating gene expression and maintaining cellular health. Understanding its precise function in these pathways will provide deeper insights into cell regulation and development.

Associated diseases and disorders

Researchers link C16orf70 to conditions like cancer and chronic obstructive pulmonary disease (COPD). The protein's altered expression or function may interfere with cellular pathways highlighting associations with oncogenic or inflammatory processes. Studies investigate its connection with proteins involved in these disorders aiming to uncover potential therapeutic targets or biomarkers.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Immunoprecipitation - Anti-C16orf70 antibody [EPR14035] - BSA and Azide free (ab250554), expandable thumbnail

    Immunoprecipitation - Anti-C16orf70 antibody [EPR14035] - BSA and Azide free (ab250554)

    This data was developed using Anti-C16orf70 antibody [EPR14035] ab181987, the same antibody clone in a different buffer formulation.Immunoprecipitation of SH-SY5Y cell lysate, labeling C16orf70 usinf Anti-C16orf70 antibody [EPR14035] ab181987 at a 1/30 dilution (lane 1) or a negative control (lane 2). Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at a 1/5000 dilution.

    All lanes: Immunoprecipitation - Anti-C16orf70 antibody [EPR14035] (Anti-C16orf70 antibody [EPR14035] ab181987)

    Predicted band size: 48 kDa

  • Western blot - Anti-C16orf70 antibody [EPR14035] - BSA and Azide free (ab250554), expandable thumbnail

    Western blot - Anti-C16orf70 antibody [EPR14035] - BSA and Azide free (ab250554)

    This data was developed using Anti-C16orf70 antibody [EPR14035] ab181987, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-C16orf70 antibody [EPR14035] (Anti-C16orf70 antibody [EPR14035] ab181987) at 1/1000 dilution

    Lane 1: Human fetal brain cell lysate at 20 µg

    Lane 2: U87-MG cell lysate at 20 µg

    Lane 3: SH-SY5Y cell lysate at 20 µg

    Lane 4: JAR cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 48 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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