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Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
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Rabbit Polyclonal C18orf22/RBFA antibody. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human RBFA aa 200 to C-terminus.
IgG
Rabbit
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Liquid
Polyclonal
WB | IHC-P | |
---|---|---|
Human | Tested | Tested |
Mouse | Tested | Expected |
Rat | Tested | Expected |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/500.00000 - 1/5000.00000 | Notes - |
Species Mouse | Dilution info 1/500.00000 - 1/5000.00000 | Notes - |
Species Rat | Dilution info 1/500.00000 - 1/5000.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/20.00000 - 1/200.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
C18orf22, RBFA
Rabbit Polyclonal C18orf22/RBFA antibody. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human RBFA aa 200 to C-terminus.
C18orf22, RBFA
IgG
Rabbit
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Liquid
Polyclonal
Affinity purification Protein G
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
C18orf22/RBFA plays an important role in the maturation of mitochondrial ribosomal RNA (rRNA). It is a component of the mitochondrial ribosomal assembly complex interacting modularly with pre-ribosomal particles. Through this interaction C18orf22/RBFA ensures the proper folding and processing of rRNA facilitating correct ribosome biogenesis. This process is important for maintaining the functionality of mitochondrial protein synthesis.
C18orf22 also known as RBFA is a mitochondrial ribosome-associated GTPase with a molecular mass of around 30 kDa. It localizes mainly in the mitochondrial matrix and functions in the stabilization and assembly of mitochondrial ribosomal subunits. C18orf22 is expressed in various tissues with a high presence in energy-demanding organs including the heart and skeletal muscle. The protein assists in the effective biogenesis of mitochondrial ribosomes which are essential for protein synthesis within mitochondria.
C18orf22/RBFA contributes significantly to the mitochondrial translation pathway. It interacts closely with other elements of the ribosomal machinery such as the mitochondrial transcription termination factor mTERF. This protein also intersects with various aspects of the oxidative phosphorylation pathway which is pivotal for ATP production in the cells. C18orf22/RBFA's role within these pathways highlights its importance in cellular energy metabolism.
Mutations or dysregulations in C18orf22/RBFA have been implicated in mitochondrial disorders especially those affecting energy metabolism such as mitochondrial myopathy. There is a connection with proteins like ND4L which plays a role in complex I of the mitochondrial electron transport chain. Furthermore research shows associations of C18orf22/RBFA with neurodegenerative diseases such as Parkinson's disease. These links suggest that dysfunctional C18orf22/RBFA can disrupt proper mitochondrial functions contributing to disease pathogenesis.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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Terms & Conditions.
All lanes: Western blot - Anti-C18orf22/RBFA antibody (AB224741) at 1/500 dilution
Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2: A549 (human lung carcinoma cell line) whole cell lysate
Lane 3: HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 4: Mouse brain tissue lysate
Lane 5: Rat heart tissue lysate
All lanes: Goat polyclonal to rabbit IgG at 1/50000 dilution
Developed using the ECL technique.
Predicted band size: 38 kDa
Paraffin embedded human ovarian cancer tissue stained for C18orf22/RBFA with ab224741 (1/100 dilution) in immunohistochemical analysis.
Paraffin embedded human lung tissue stained for C18orf22/RBFA with ab224741 (1/100 dilution) in immunohistochemical analysis.
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