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Rabbit Polyclonal C18orf22/RBFA antibody. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human RBFA aa 200 to C-terminus.

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Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human RBFA aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q8N0V3
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Human
Tested
Tested
Mouse
Tested
Expected
Rat
Tested
Expected

Tested
Tested

Species

Human

Dilution info

1/500.00000 - 1/5000.00000

Notes

-

Species

Mouse

Dilution info

1/500.00000 - 1/5000.00000

Notes

-

Species

Rat

Dilution info

1/500.00000 - 1/5000.00000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/20.00000 - 1/200.00000

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Alternative names

Recommended products

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Rabbit Polyclonal C18orf22/RBFA antibody. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human RBFA aa 200 to C-terminus.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human RBFA aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q8N0V3
Purification technique

Affinity purification Protein G

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

Biological function summary

C18orf22/RBFA plays an important role in the maturation of mitochondrial ribosomal RNA (rRNA). It is a component of the mitochondrial ribosomal assembly complex interacting modularly with pre-ribosomal particles. Through this interaction C18orf22/RBFA ensures the proper folding and processing of rRNA facilitating correct ribosome biogenesis. This process is important for maintaining the functionality of mitochondrial protein synthesis.

Activity summary

C18orf22 also known as RBFA is a mitochondrial ribosome-associated GTPase with a molecular mass of around 30 kDa. It localizes mainly in the mitochondrial matrix and functions in the stabilization and assembly of mitochondrial ribosomal subunits. C18orf22 is expressed in various tissues with a high presence in energy-demanding organs including the heart and skeletal muscle. The protein assists in the effective biogenesis of mitochondrial ribosomes which are essential for protein synthesis within mitochondria.

Pathways

C18orf22/RBFA contributes significantly to the mitochondrial translation pathway. It interacts closely with other elements of the ribosomal machinery such as the mitochondrial transcription termination factor mTERF. This protein also intersects with various aspects of the oxidative phosphorylation pathway which is pivotal for ATP production in the cells. C18orf22/RBFA's role within these pathways highlights its importance in cellular energy metabolism.

Associated diseases and disorders

Mutations or dysregulations in C18orf22/RBFA have been implicated in mitochondrial disorders especially those affecting energy metabolism such as mitochondrial myopathy. There is a connection with proteins like ND4L which plays a role in complex I of the mitochondrial electron transport chain. Furthermore research shows associations of C18orf22/RBFA with neurodegenerative diseases such as Parkinson's disease. These links suggest that dysfunctional C18orf22/RBFA can disrupt proper mitochondrial functions contributing to disease pathogenesis.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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3 product images

  • Western blot - Anti-C18orf22/RBFA antibody (ab224741), expandable thumbnail

    Western blot - Anti-C18orf22/RBFA antibody (ab224741)

    All lanes: Western blot - Anti-C18orf22/RBFA antibody (AB224741) at 1/500 dilution

    Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

    Lane 2: A549 (human lung carcinoma cell line) whole cell lysate

    Lane 3: HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate

    Lane 4: Mouse brain tissue lysate

    Lane 5: Rat heart tissue lysate

    Secondary

    All lanes: Goat polyclonal to rabbit IgG at 1/50000 dilution

    Developed using the ECL technique.

    Predicted band size: 38 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C18orf22/RBFA antibody (ab224741), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C18orf22/RBFA antibody (ab224741)

    Paraffin embedded human ovarian cancer tissue stained for C18orf22/RBFA with ab224741 (1/100 dilution) in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C18orf22/RBFA antibody (ab224741), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C18orf22/RBFA antibody (ab224741)

    Paraffin embedded human lung tissue stained for C18orf22/RBFA with ab224741 (1/100 dilution) in immunohistochemical analysis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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