Rabbit Polyclonal C19orf21 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human MISP aa 550 to C-terminus.
Images
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human MISP aa 550 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q8IVT2
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2500.00000 - 1/5000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100 |
Target data
Function
Plays a role in mitotic spindle orientation and mitotic progression. Regulates the distribution of dynactin at the cell cortex in a PLK1-dependent manner, thus stabilizing cortical and astral microtubule attachments required for proper mitotic spindle positioning. May link microtubules to the actin cytospkeleton and focal adhesions. May be required for directed cell migration and centrosome orientation. May also be necessary for proper stacking of the Golgi apparatus.
Alternative names
Mitotic interactor and substrate of PLK1, Mitotic spindle positioning protein, MISP, C19orf21
Recommended products
Rabbit Polyclonal C19orf21 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human MISP aa 550 to C-terminus.
Alternative names
Mitotic interactor and substrate of PLK1, Mitotic spindle positioning protein, MISP, C19orf21
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human MISP aa 550 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q8IVT2
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
C19orf21 also known as Chromosome 19 Open Reading Frame 21 is a protein with a molecular mass of approximately 26 kDa. It is expressed in various tissues with significant activity in the spleen and lung. Mechanically C19orf21 functions in nuclear processes including transcription regulation and chromatin remodeling. Due to its involvement in these activities C19orf21 interacts with nuclear components and may impact gene expression by influencing chromatin structure.
- Biological function summary
C19orf21 plays a role in regulating cellular homeostasis and proliferation. C19orf21 is part of the chromatin remodeling complex which modifies chromatin to allow access to transcription machinery. This participation suggests importance in controlling gene accessibility. C19orf21's involvement in such intricate processes indicates a possible role in cellular development and differentiation influencing tissue-specific gene expression.
- Pathways
C19orf21 is involved in chromatin modification and transcriptional regulation pathways. It interacts with proteins like histone acetyltransferases contributing to epigenetic modifications and regulation of gene expression. Additionally C19orf21 shows involvement in pathways that maintain DNA integrity working alongside proteins like DNA repair enzymes. These interactions suggest that C19orf21 supports cellular responses to damage and stress.
- Associated diseases and disorders
C19orf21 has been implicated in certain types of cancer and neurological disorders. In cancer it relates to transcription dysregulation potentially interacting with oncogenic factors. Aberrant expression or mutation of C19orf21 may disrupt normal gene regulation leading to tumorigenesis. In neurological disorders C19orf21's role in chromatin remodeling could affect gene expression patterns associated with neural function linking it to conditions such as Alzheimer's disease. In these contexts C19orf21 may interact with known proteins involved such as beta-amyloid precursor protein in Alzheimer's suggesting a wider implication in disease mechanisms.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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6 product images
Immunocytochemistry/ Immunofluorescence - Anti-C19orf21 antibody (ab254919)
PFA fixed, Triton X-100 permeabilized U-2 OS (Human bone osteosarcoma epithelial cell line) cells labeling C19orf21 using ab254919 at 4µg/ml (green) in ICC/IF.
Western blot - Anti-C19orf21 antibody (ab254919)
All lanes: Western blot - Anti-C19orf21 antibody (ab254919) at 0.4 µg/mL
Lane 1: Vector only transfected HEK-293T cell lysate
Lane 2: C19orf21 overexpressed HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Predicted band size: 75 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C19orf21 antibody (ab254919)
Paraffin-embedded human small intestine tissue stained for C19orf21 using ab254919 at 1/2500 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C19orf21 antibody (ab254919)
Paraffin-embedded human kidney tissue stained for C19orf21 using ab254919 at 1/2500 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C19orf21 antibody (ab254919)
Paraffin-embedded human testis tissue stained for C19orf21 using ab254919 at 1/2500 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C19orf21 antibody (ab254919)
Paraffin-embedded human liver tissue stained for C19orf21 using ab254919 at 1/2500 dilution in immunohistochemical analysis.
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