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AB254919

Anti-C19orf21 antibody

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(2 Publications)

Rabbit Polyclonal C19orf21 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MISP aa 550 to C-terminus.

View Alternative Names

C19orf21, MISP, Mitotic interactor and substrate of PLK1, Mitotic spindle positioning protein

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C19orf21 antibody (AB254919)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C19orf21 antibody (AB254919)

Paraffin-embedded human kidney tissue stained for C19orf21 using ab254919 at 1/2500 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-C19orf21 antibody (AB254919)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-C19orf21 antibody (AB254919)

PFA fixed, Triton X-100 permeabilized U-2 OS (Human bone osteosarcoma epithelial cell line) cells labeling C19orf21 using ab254919 at 4µg/ml (green) in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C19orf21 antibody (AB254919)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C19orf21 antibody (AB254919)

Paraffin-embedded human testis tissue stained for C19orf21 using ab254919 at 1/2500 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C19orf21 antibody (AB254919)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C19orf21 antibody (AB254919)

Paraffin-embedded human liver tissue stained for C19orf21 using ab254919 at 1/2500 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C19orf21 antibody (AB254919)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C19orf21 antibody (AB254919)

Paraffin-embedded human small intestine tissue stained for C19orf21 using ab254919 at 1/2500 dilution in immunohistochemical analysis.

Western blot - Anti-C19orf21 antibody (AB254919)
  • WB

Supplier Data

Western blot - Anti-C19orf21 antibody (AB254919)

All lanes:

Western blot - Anti-C19orf21 antibody (ab254919) at 0.4 µg/mL

Lane 1:

Vector only transfected HEK-293T cell lysate

Lane 2:

C19orf21 overexpressed HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

Predicted band size: 75 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human MISP aa 550 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q8IVT2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2500 - 1/5000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

C19orf21 also known as Chromosome 19 Open Reading Frame 21 is a protein with a molecular mass of approximately 26 kDa. It is expressed in various tissues with significant activity in the spleen and lung. Mechanically C19orf21 functions in nuclear processes including transcription regulation and chromatin remodeling. Due to its involvement in these activities C19orf21 interacts with nuclear components and may impact gene expression by influencing chromatin structure.
Biological function summary

C19orf21 plays a role in regulating cellular homeostasis and proliferation. C19orf21 is part of the chromatin remodeling complex which modifies chromatin to allow access to transcription machinery. This participation suggests importance in controlling gene accessibility. C19orf21's involvement in such intricate processes indicates a possible role in cellular development and differentiation influencing tissue-specific gene expression.

Pathways

C19orf21 is involved in chromatin modification and transcriptional regulation pathways. It interacts with proteins like histone acetyltransferases contributing to epigenetic modifications and regulation of gene expression. Additionally C19orf21 shows involvement in pathways that maintain DNA integrity working alongside proteins like DNA repair enzymes. These interactions suggest that C19orf21 supports cellular responses to damage and stress.

C19orf21 has been implicated in certain types of cancer and neurological disorders. In cancer it relates to transcription dysregulation potentially interacting with oncogenic factors. Aberrant expression or mutation of C19orf21 may disrupt normal gene regulation leading to tumorigenesis. In neurological disorders C19orf21's role in chromatin remodeling could affect gene expression patterns associated with neural function linking it to conditions such as Alzheimer's disease. In these contexts C19orf21 may interact with known proteins involved such as beta-amyloid precursor protein in Alzheimer's suggesting a wider implication in disease mechanisms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in mitotic spindle orientation and mitotic progression. Regulates the distribution of dynactin at the cell cortex in a PLK1-dependent manner, thus stabilizing cortical and astral microtubule attachments required for proper mitotic spindle positioning. May link microtubules to the actin cytospkeleton and focal adhesions. May be required for directed cell migration and centrosome orientation. May also be necessary for proper stacking of the Golgi apparatus.
See full target information MISP
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Biomedical research (Tokyo, Japan) 46:93-99 PubMed40436766

2025

The predictive value of MISP for the postoperative outcomes of gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Jin,Lei Xin,Hao Wang,Yunpeng Lin,Yan Zhou

Oncology letters 22:532 PubMed34079591

2021

miR-590-5p targets RMND5A and promotes migration in pancreatic adenocarcinoma cell lines.

Applications

Unspecified application

Species

Unspecified reactive species

Sixing Chen,Yu Chen,Yao Wen,Wanwan Cai,Ping Zhu,Wuzhou Yuan,Yongqing Li,Xiongwei Fan,Yongqi Wan,Fang Li,Jian Zhuang,Zhigang Jiang,Xiushan Wu,Yuequn Wang
View all publications
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Product promise

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