AB185365

Anti-C19orf63 antibody

Name: Anti-C19orf63 antibody (ab185365) | Abcam
Brand: Abcam Limited
SKU: AB185365
Price: 630 USD
Availability: InStock

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Rabbit Polyclonal C19orf63 antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human EMC10 aa 100-250.

View Alternative Names

C19orf63, INM02, UNQ764/PRO1556, EMC10, ER membrane protein complex subunit 10, Hematopoietic signal peptide-containing membrane domain-containing protein 1

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C19orf63 antibody (AB185365)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C19orf63 antibody (AB185365)

Immunohistochemical analysisng of human testis tissue labeling C19orf63 in the cytoplasm of Leydig cells with ab185365 at a 1/50 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C19orf63 antibody (AB185365)

Immunohistochemical analysisng of human liver tissue labeling C19orf63 in the membrane of hepatocytes with ab185365 at a 1/50 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C19orf63 antibody (AB185365)

Immunohistochemical analysisng of human skeletal muscle tissue labeling C19orf63 in the cytoplasm of myocytes with ab185365 at a 1/50 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C19orf63 antibody (AB185365)

Immunohistochemical analysisng of human Fallopian tube tissue labeling C19orf63 in the apical membrane of glandular cells with ab185365 at a 1/50 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-C19orf63 antibody (AB185365)

All lanes:

Western blot - Anti-C19orf63 antibody (ab185365) at 0.4 µg/mL

All lanes:

SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate

Predicted band size: 27 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human EMC10 aa 100-250. The exact immunogen used to generate this antibody is proprietary information.

Q5UCC4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The C19orf63 protein also known as MRG domain-binding protein (MRGBP) works mechanically as a component of the histone acetyltransferase complex. It weighs approximately 19 kDa and is expressed in various human tissues with notable expression in HeLa cells. This protein is integral to chromatin modifications influencing transcription regulation through histone acetylation.

Biological function summary

C19orf63 interacts within the histone acetyltransferase complex playing a role in the modification of chromatin structure. It participates in transcriptional regulation by modulating the acetylation state of histones thereby impacting gene expression. This protein does not work alone and requires interaction with other proteins to execute its function efficiently.

Pathways

C19orf63 operates in pathways related to chromatin modification and transcription control. The protein influences the regulation of transcriptional signaling pathways such as the acetylation-dependent signaling pathway. C19orf63 associates with proteins like PCAF a known histone acetyltransferase working together to maintain gene accessibility and transcription fidelity.

C19orf63 has connections with certain cancers through its role in chromatin structure and function alteration. Abnormal expression of this protein might contribute to tumorigenesis by altering gene expression profiles. Through the histone modification pathway it connects with proteins like BRCA1 which is involved in DNA repair and is associated with breast cancer.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Part of the endoplasmic reticulum membrane protein complex (EMC) that enables the energy-independent insertion into endoplasmic reticulum membranes of newly synthesized membrane proteins (PubMed : 29242231, PubMed : 29809151, PubMed : 30415835, PubMed : 32439656, PubMed : 32459176). Preferentially accommodates proteins with transmembrane domains that are weakly hydrophobic or contain destabilizing features such as charged and aromatic residues (PubMed : 29242231, PubMed : 29809151, PubMed : 30415835). Involved in the cotranslational insertion of multi-pass membrane proteins in which stop-transfer membrane-anchor sequences become ER membrane spanning helices (PubMed : 29809151, PubMed : 30415835). It is also required for the post-translational insertion of tail-anchored/TA proteins in endoplasmic reticulum membranes (PubMed : 29242231, PubMed : 29809151). By mediating the proper cotranslational insertion of N-terminal transmembrane domains in an N-exo topology, with translocated N-terminus in the lumen of the ER, controls the topology of multi-pass membrane proteins like the G protein-coupled receptors (PubMed : 30415835). By regulating the insertion of various proteins in membranes, it is indirectly involved in many cellular processes (Probable). Promotes angiogenesis and tissue repair in the heart after myocardial infarction. Stimulates cardiac endothelial cell migration and outgrowth via the activation of p38 MAPK, PAK and MAPK2 signaling pathways (PubMed : 28931551).

See full target information EMC10

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-C19orf63 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C19orf63 antibody (AB185365)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C19orf63 antibody (AB185365)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C19orf63 antibody (AB185365)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C19orf63 antibody (AB185365)

Western blot - Anti-C19orf63 antibody (AB185365)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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