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AB180148

Anti-C19orf63 antibody [EPR13223-65]

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(4 Publications)

Rabbit Recombinant Monoclonal C19orf63 antibody. Suitable for WB and reacts with Human samples. Cited in 4 publications.

View Alternative Names

C19orf63, INM02, UNQ764/PRO1556, EMC10, ER membrane protein complex subunit 10, Hematopoietic signal peptide-containing membrane domain-containing protein 1

2 Images
Western blot - Anti-C19orf63 antibody [EPR13223-65] (AB180148)
  • WB

Lab

Western blot - Anti-C19orf63 antibody [EPR13223-65] (AB180148)

Lanes 1-3 : Merged signal (red and green). Green - ab180148 observed at 27 kDa. Red - loading control ab7291 observed at 50 kDa.

ab180148 Anti-C19orf63 antibody [EPR13223-65] was shown to specifically react with C19orf63 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265783 (knockout cell lysate ab257939) was used. Wild-type and C19orf63 knockout samples were subjected to SDS-PAGE. ab180148 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-C19orf63 antibody [EPR13223-65] (ab180148) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

EMC10 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human EMC10 (C19orf63) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-emc10-c19orf63-knockout-hela-cell-line-ab265783'>ab265783</a>)

Lane 3:

A549 cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 27 kDa

Observed band size: 27 kDa

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Western blot - Anti-C19orf63 antibody [EPR13223-65] (AB180148)
  • WB

Supplier Data

Western blot - Anti-C19orf63 antibody [EPR13223-65] (AB180148)

All lanes:

Western blot - Anti-C19orf63 antibody [EPR13223-65] (ab180148) at 1/1000 dilution

Lane 1:

A549 cell lysate at 20 µg

Lane 2:

293 cell lysate at 20 µg

Lane 3:

U87-MG cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 27 kDa

false

  • Carrier free

    Anti-C19orf63 antibody [EPR13223-65] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13223-65

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The C19orf63 protein also known as MRG domain-binding protein (MRGBP) works mechanically as a component of the histone acetyltransferase complex. It weighs approximately 19 kDa and is expressed in various human tissues with notable expression in HeLa cells. This protein is integral to chromatin modifications influencing transcription regulation through histone acetylation.
Biological function summary

C19orf63 interacts within the histone acetyltransferase complex playing a role in the modification of chromatin structure. It participates in transcriptional regulation by modulating the acetylation state of histones thereby impacting gene expression. This protein does not work alone and requires interaction with other proteins to execute its function efficiently.

Pathways

C19orf63 operates in pathways related to chromatin modification and transcription control. The protein influences the regulation of transcriptional signaling pathways such as the acetylation-dependent signaling pathway. C19orf63 associates with proteins like PCAF a known histone acetyltransferase working together to maintain gene accessibility and transcription fidelity.

C19orf63 has connections with certain cancers through its role in chromatin structure and function alteration. Abnormal expression of this protein might contribute to tumorigenesis by altering gene expression profiles. Through the histone modification pathway it connects with proteins like BRCA1 which is involved in DNA repair and is associated with breast cancer.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Part of the endoplasmic reticulum membrane protein complex (EMC) that enables the energy-independent insertion into endoplasmic reticulum membranes of newly synthesized membrane proteins (PubMed : 29242231, PubMed : 29809151, PubMed : 30415835, PubMed : 32439656, PubMed : 32459176). Preferentially accommodates proteins with transmembrane domains that are weakly hydrophobic or contain destabilizing features such as charged and aromatic residues (PubMed : 29242231, PubMed : 29809151, PubMed : 30415835). Involved in the cotranslational insertion of multi-pass membrane proteins in which stop-transfer membrane-anchor sequences become ER membrane spanning helices (PubMed : 29809151, PubMed : 30415835). It is also required for the post-translational insertion of tail-anchored/TA proteins in endoplasmic reticulum membranes (PubMed : 29242231, PubMed : 29809151). By mediating the proper cotranslational insertion of N-terminal transmembrane domains in an N-exo topology, with translocated N-terminus in the lumen of the ER, controls the topology of multi-pass membrane proteins like the G protein-coupled receptors (PubMed : 30415835). By regulating the insertion of various proteins in membranes, it is indirectly involved in many cellular processes (Probable). Promotes angiogenesis and tissue repair in the heart after myocardial infarction. Stimulates cardiac endothelial cell migration and outgrowth via the activation of p38 MAPK, PAK and MAPK2 signaling pathways (PubMed : 28931551).
See full target information EMC10
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

The Journal of cell biology 222: PubMed37199759

2023

A selectivity filter in the ER membrane protein complex limits protein misinsertion at the ER.

Applications

Unspecified application

Species

Unspecified reactive species

Tino Pleiner,Masami Hazu,Giovani Pinton Tomaleri,Vy N Nguyen,Kurt Januszyk,Rebecca M Voorhees

eLife 10: PubMed34414886

2021

An engineered transcriptional reporter of protein localization identifies regulators of mitochondrial and ER membrane protein trafficking in high-throughput CRISPRi screens.

Applications

Unspecified application

Species

Unspecified reactive species

Robert Coukos,David Yao,Mateo I Sanchez,Eric T Strand,Meagan E Olive,Namrata D Udeshi,Jonathan S Weissman,Steven A Carr,Michael C Bassik,Alice Y Ting

eLife 9: PubMed33236988

2020

Structural and mechanistic basis of the EMC-dependent biogenesis of distinct transmembrane clients.

Applications

Unspecified application

Species

Unspecified reactive species

Lakshmi E Miller-Vedam,Bastian Bräuning,Katerina D Popova,Nicole T Schirle Oakdale,Jessica L Bonnar,Jesuraj R Prabu,Elizabeth A Boydston,Natalia Sevillano,Matthew J Shurtleff,Robert M Stroud,Charles S Craik,Brenda A Schulman,Adam Frost,Jonathan S Weissman

Journal of cell science 132: PubMed30578317

2019

The ER membrane protein complex promotes biogenesis of sterol-related enzymes maintaining cholesterol homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Norbert Volkmar,Maria-Laetitia Thezenas,Sharon M Louie,Szymon Juszkiewicz,Daniel K Nomura,Ramanujan S Hegde,Benedikt M Kessler,John C Christianson
View all publications
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Product promise

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