AB122765

Anti-C19orf66 antibody

Name: Anti-C19orf66 antibody (ab122765) | Abcam
Brand: Abcam Limited
SKU: AB122765

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(3 Publications)

Rabbit Polyclonal C19orf66 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SHFL aa 1-150.

View Alternative Names

C19orf66, FLJ11286, IRAV, RYDEN, SFL, SHFL, Shiftless antiviral inhibitor of ribosomal frameshifting protein, Interferon-regulated antiviral protein, Repressor of yield of DENV protein, RyDEN

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C19orf66 antibody (AB122765)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C19orf66 antibody (AB122765)

Immunohistochemical analysis of human liver tissue labeling C19orf66 in the cytoplasm of hepatocytes with ab122765 at a 1/200 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C19orf66 antibody (AB122765)

Immunohistochemical analysis of human testis tissue labeling C19orf66 in the cytoplasm of Leydig cells and of cells in seminiferous ducts with ab122765 at a 1/200 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-C19orf66 antibody (AB122765)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-C19orf66 antibody (AB122765)

Immunocytochemical analysis of U-2 OS (Human bone osteosarcoma epithelial cell line) whole cells labeling C19orf66 in the nucleoplasm with ab122765 at 2 μg/ml.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C19orf66 antibody (AB122765)

Immunohistochemical analysis of human Fallopian tube tissue labeling C19orf66 in the cytoplasm of glandular cells with ab122765 at a 1/200 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C19orf66 antibody (AB122765)

Immunohistochemical analysis of human prostate tissue labeling C19orf66 in the cytoplasm of glandular cells with ab122765 at a 1/200 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human SHFL aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

Q9NUL5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

C19orf66 also known as Shiftless or C6orf166 is a protein with a molecular mass of approximately 27 kDa. It functions as an inhibitor of viral RNA replication. Research shows that C19orf66 disrupts viral protein synthesis by interacting with viral factors and initiating their degradation making it important for antiviral defense. This protein is expressed in various tissues with high levels detected in immune-related cells suggesting its role in innate immune response.

Biological function summary

C19orf66 plays an essential role in modulating immune response against viral pathogens. Its action involves the inhibition of viral replication which helps in controlling the spread of the infection within the host. C19orf66 does not typically form part of large protein complexes but it exerts its effect through direct interaction with viral components preventing their proper assembly and function.

Pathways

C19orf66 is involved in important antiviral defense mechanisms including those that overlap with the interferon signaling pathway. This protein interacts with interferon-stimulated genes enhancing the host's capability to limit viral infections. Although not directly part of an extensive pathway like NF-kB or MAPK C19orf66 contributes to antiviral defense through its relationship with proteins that are engaged by these pathways during immune responses.

C19orf66 shows implications in viral infections and may play a role in diseases where the immune response is compromised such as chronic viral infections. It has a functional connection with viral proteins which aids in limiting the replication and persistence of viruses. Studies explore its potential usefulness as a therapeutic target especially considering its ability to inhibit the replication of various viruses without directly impacting host cellular machinery.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Inhibits programmed -1 ribosomal frameshifting (-1PRF) of a variety of mRNAs from viruses, such as HIV1, and cellular genes, such as PEG10. Interacts with the -1PRF signal of target mRNA and translating ribosomes and causes premature translation termination at the frameshifting site (PubMed : 30682371). Regulates HIV1 GAG-POL expression by inhibiting -1PRF (PubMed : 30682371). Exhibits antiviral activity against dengue virus (DENV) and can inhibit the replication of all DENV serotypes. May block the protein translation of DENV RNA via its association with cellular mRNA-binding proteins and viral RNA. Also interrupts Zika virus replication by promoting viral NS3 degradation via a lysosome-dependent pathway (PubMed : 32150556). Can also limit the replication of hepatitis C virus (HCV) by restricting formation of viral replication organelle, West Nile virus (WNV), Chikungunya virus (CHIKV), herpes simplex virus type 1 (HHV-1), herpes virus type 8 (HHV-8) and human adenovirus (PubMed : 26735137, PubMed : 27974568, PubMed : 30944177, PubMed : 32294532). Binds nucleic acids with a higher affinity for ssRNA and ssDNA than for dsDNA (PubMed : 27974568).. Isoform 4 does not inhibit programmed ribosomal frameshifting (-1PRF). Does not bind to ribosomes.

See full target information SHFL

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 15:1347948 PubMed38370417

2024

IgLON5 deficiency produces behavioral alterations in a knockout mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Jon Landa,Ana Beatriz Serafim,Mercedes Alba,Estibaliz Maudes,Laura Molina-Porcel,Anna Garcia-Serra,Francesco Mannara,Josep Dalmau,Francesc Graus,Lidia Sabater

Molecular cell 81:2838-2850.e6 PubMed33989516

2021

The SARS-CoV-2 RNA interactome.

Applications

Unspecified application

Species

Unspecified reactive species

Sungyul Lee,Young-Suk Lee,Yeon Choi,Ahyeon Son,Youngran Park,Kyung-Min Lee,Jeesoo Kim,Jong-Seo Kim,V Narry Kim

Cell 176:625-635.e14 PubMed30682371

2019

Regulation of HIV-1 Gag-Pol Expression by Shiftless, an Inhibitor of Programmed -1 Ribosomal Frameshifting.

Applications

Unspecified application

Species

Unspecified reactive species

Xinlu Wang,Yifang Xuan,Yuling Han,Xiang Ding,Kai Ye,Fuquan Yang,Pu Gao,Stephen P Goff,Guangxia Gao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-C19orf66 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C19orf66 antibody (AB122765)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C19orf66 antibody (AB122765)

Immunocytochemistry/ Immunofluorescence - Anti-C19orf66 antibody (AB122765)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C19orf66 antibody (AB122765)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C19orf66 antibody (AB122765)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise