Rabbit Polyclonal C1GALT1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human C1GALT1 aa 1-150.
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Publications
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- American journal of nephrology 53:10-202021Development of Urinary Diagnostic Biomarker for IgA Nephropathy by Lectin Microarray.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYasuhiro Onishi,Koki Mise,Chieko Kawakita,Haruhito A Uchida,Hitoshi Sugiyama,Ryosuke Sugawara,Satoshi Yamaguchi,Michihiro Yoshida,Toshiharu Mitsuhashi,Masao Yamada,Jun Hirabayashi,Jun WadaPubMed 34965524
- Cell & bioscience 11:1662021A novel mechanism for C1GALT1 in the regulation of gastric cancer progression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaoxia Dong,Chunli Chen,Xinzhou Deng,Yongyu Liu,Qiwen Duan,Zhen Peng,Zhiguo Luo,Li ShenPubMed 34452648
- Frontiers in cell and developmental biology 9:7079702021C1GALT1, Negatively Regulated by miR-181d-5p, Promotes Tumor Progression via Upregulating RAC1 in Lung Adenocarcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaoxia Dong,Yongyu Liu,Xinzhou Deng,Jun Shao,Shuangyue Tian,Shuang Chen,Rongxin Huang,Ziao Lin,Chunli Chen,Li ShenPubMed 34307388
- Frontiers in immunology 11:5636992020microRNA-630 Regulates Underglycosylated IgA1 Production in the Tonsils by Targeting TLR4 in IgA Nephropathy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChan Liu et. al.PubMed 33324395
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human C1GALT1 aa 1-150. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NS00
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/5000.00000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/500.00000 | Notes - |
Target data
Function
Glycosyltransferase that generates the core 1 O-glycan Gal-beta1-3GalNAc-alpha1-Ser/Thr (T antigen), which is a precursor for many extended O-glycans in glycoproteins. Plays a central role in many processes, such as angiogenesis, thrombopoiesis and kidney homeostasis development.
Alternative names
Glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase 1, B3Gal-T8, Core 1 O-glycan T-synthase, C1GalT1, Core 1 beta3-Gal-T1, C1GALT1
Recommended products
Rabbit Polyclonal C1GALT1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human C1GALT1 aa 1-150.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human C1GALT1 aa 1-150. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NS00
- Purification technique
Affinity purification Protein G
- Concentration
- Purification notes
Purity >95%.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
C1GALT1 also known as core 1 β13-galactosyltransferase is an enzyme important for the synthesis of core 1 O-glycans. The enzyme has a molecular mass of approximately 55 kDa. It localizes in the Golgi apparatus where it actively participates in glycosylation processes. C1GALT1 shows widespread expression in various tissues including the liver lungs and intestines. By catalyzing the transfer of galactose to N-acetylgalactosamine on proteins it plays an important role in O-glycan biosynthesis.
- Biological function summary
The function of C1GALT1 extends to modifying proteins that impact cell-cell interactions protein stability and signaling pathways. C1GALT1 does not work alone but as a part of a complex with chaperone protein COSMC which is necessary for its glycosyltransferase activity. This complex formation is essential to ensure the proper structure and function of the resulting glycoproteins which have a broad range of impacts in cell biology and communication.
- Pathways
C1GALT1 plays a significant role in the mucin-type O-glycan biosynthesis pathway which is critical for producing mucins. These proteins are important for protective mucus barriers in several organs. C1GALT1 shows interaction with galectin proteins that recognize specific glycan structures influencing cellular processes such as adhesion and migration. Additionally it participates in the PI3K-Akt signaling pathway which links to cell growth and survival often intersecting with pathways involving other glycosyltransferases.
- Associated diseases and disorders
C1GALT1 has associations with IgA nephropathy also known as Berger's disease where abnormal glycosylation patterns of IgA1 occur. This disorder often involves the misfunction of proteins like C1GALT1 and COSMC. Furthermore aberrant C1GALT1 activity may have links to cancer progression as improper glycosylation can impact tumor cell migration and invasion. C1GALT1's interplay with galectins and other glycoproteins influences these pathological conditions making it a focal point for understanding and potentially targeting these diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Western blot - Anti-C1GALT1 antibody (ab237734)
All lanes: Western blot - Anti-C1GALT1 antibody (ab237734) at 1/500 dilution
Lane 1: U-251 MG (human brain glioma cell line) whole cell lysate
Lane 2: HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
SecondaryAll lanes: Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 42 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1GALT1 antibody (ab237734)
Paraffin-embedded human colon cancer tissue stained for C1GALT1 using ab237734 at 1/1000 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1GALT1 antibody (ab237734)
Paraffin-embedded human kidney tissue stained for C1GALT1 using ab237734 at 1/1000 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunocytochemistry/ Immunofluorescence - Anti-C1GALT1 antibody (ab237734)
HepG2 (human liver hepatocellular carcinoma cell line) cells stained for C1GALT1 (green) using ab237734 at 1/333 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L). The nuclear counter stain is DAPI.
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.
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